Cargando…

Hydrolytic Degradation and Mechanical Stability of Poly(ε-Caprolactone)/Reduced Graphene Oxide Membranes as Scaffolds for In Vitro Neural Tissue Regeneration

The present work studies the functional behavior of novel poly(ε-caprolactone) (PCL) membranes functionalized with reduced graphene oxide (rGO) nanoplatelets under simulated in vitro culture conditions (phosphate buffer solution (PBS) at 37 °C) during 1 year, in order to elucidate their applicabilit...

Descripción completa

Detalles Bibliográficos
Autores principales: Sánchez-González, Sandra, Diban, Nazely, Urtiaga, Ane
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5872194/
https://www.ncbi.nlm.nih.gov/pubmed/29510552
http://dx.doi.org/10.3390/membranes8010012
_version_ 1783309782757670912
author Sánchez-González, Sandra
Diban, Nazely
Urtiaga, Ane
author_facet Sánchez-González, Sandra
Diban, Nazely
Urtiaga, Ane
author_sort Sánchez-González, Sandra
collection PubMed
description The present work studies the functional behavior of novel poly(ε-caprolactone) (PCL) membranes functionalized with reduced graphene oxide (rGO) nanoplatelets under simulated in vitro culture conditions (phosphate buffer solution (PBS) at 37 °C) during 1 year, in order to elucidate their applicability as scaffolds for in vitro neural regeneration. The morphological, chemical, and DSC results demonstrated that high internal porosity of the membranes facilitated water permeation and procured an accelerated hydrolytic degradation throughout the bulk pathway. Therefore, similar molecular weight reduction, from 80 kDa to 33 kDa for the control PCL, and to 27 kDa for PCL/rGO membranes, at the end of the study, was observed. After 1 year of hydrolytic degradation, though monomers coming from the hydrolytic cleavage of PCL diffused towards the PBS medium, the pH was barely affected, and the rGO nanoplatelets mainly remained in the membranes which envisaged low cytotoxic effect. On the other hand, the presence of rGO nanomaterials accelerated the loss of mechanical stability of the membranes. However, it is envisioned that the gradual degradation of the PCL/rGO membranes could facilitate cells infiltration, interconnectivity, and tissue formation.
format Online
Article
Text
id pubmed-5872194
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-58721942018-03-30 Hydrolytic Degradation and Mechanical Stability of Poly(ε-Caprolactone)/Reduced Graphene Oxide Membranes as Scaffolds for In Vitro Neural Tissue Regeneration Sánchez-González, Sandra Diban, Nazely Urtiaga, Ane Membranes (Basel) Article The present work studies the functional behavior of novel poly(ε-caprolactone) (PCL) membranes functionalized with reduced graphene oxide (rGO) nanoplatelets under simulated in vitro culture conditions (phosphate buffer solution (PBS) at 37 °C) during 1 year, in order to elucidate their applicability as scaffolds for in vitro neural regeneration. The morphological, chemical, and DSC results demonstrated that high internal porosity of the membranes facilitated water permeation and procured an accelerated hydrolytic degradation throughout the bulk pathway. Therefore, similar molecular weight reduction, from 80 kDa to 33 kDa for the control PCL, and to 27 kDa for PCL/rGO membranes, at the end of the study, was observed. After 1 year of hydrolytic degradation, though monomers coming from the hydrolytic cleavage of PCL diffused towards the PBS medium, the pH was barely affected, and the rGO nanoplatelets mainly remained in the membranes which envisaged low cytotoxic effect. On the other hand, the presence of rGO nanomaterials accelerated the loss of mechanical stability of the membranes. However, it is envisioned that the gradual degradation of the PCL/rGO membranes could facilitate cells infiltration, interconnectivity, and tissue formation. MDPI 2018-03-05 /pmc/articles/PMC5872194/ /pubmed/29510552 http://dx.doi.org/10.3390/membranes8010012 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sánchez-González, Sandra
Diban, Nazely
Urtiaga, Ane
Hydrolytic Degradation and Mechanical Stability of Poly(ε-Caprolactone)/Reduced Graphene Oxide Membranes as Scaffolds for In Vitro Neural Tissue Regeneration
title Hydrolytic Degradation and Mechanical Stability of Poly(ε-Caprolactone)/Reduced Graphene Oxide Membranes as Scaffolds for In Vitro Neural Tissue Regeneration
title_full Hydrolytic Degradation and Mechanical Stability of Poly(ε-Caprolactone)/Reduced Graphene Oxide Membranes as Scaffolds for In Vitro Neural Tissue Regeneration
title_fullStr Hydrolytic Degradation and Mechanical Stability of Poly(ε-Caprolactone)/Reduced Graphene Oxide Membranes as Scaffolds for In Vitro Neural Tissue Regeneration
title_full_unstemmed Hydrolytic Degradation and Mechanical Stability of Poly(ε-Caprolactone)/Reduced Graphene Oxide Membranes as Scaffolds for In Vitro Neural Tissue Regeneration
title_short Hydrolytic Degradation and Mechanical Stability of Poly(ε-Caprolactone)/Reduced Graphene Oxide Membranes as Scaffolds for In Vitro Neural Tissue Regeneration
title_sort hydrolytic degradation and mechanical stability of poly(ε-caprolactone)/reduced graphene oxide membranes as scaffolds for in vitro neural tissue regeneration
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5872194/
https://www.ncbi.nlm.nih.gov/pubmed/29510552
http://dx.doi.org/10.3390/membranes8010012
work_keys_str_mv AT sanchezgonzalezsandra hydrolyticdegradationandmechanicalstabilityofpolyecaprolactonereducedgrapheneoxidemembranesasscaffoldsforinvitroneuraltissueregeneration
AT dibannazely hydrolyticdegradationandmechanicalstabilityofpolyecaprolactonereducedgrapheneoxidemembranesasscaffoldsforinvitroneuraltissueregeneration
AT urtiagaane hydrolyticdegradationandmechanicalstabilityofpolyecaprolactonereducedgrapheneoxidemembranesasscaffoldsforinvitroneuraltissueregeneration