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PCR Diagnosis of Small Hive Beetles
Small hive beetles (SHBs), Aethina tumida, are parasites of social bee colonies native to sub-Saharan Africa and have become an invasive species at a global scale. Reliable Polymerase Chain Reaction (PCR) diagnosis of this mandatory pest is required to limit its further spread and impact. Here, we h...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5872289/ https://www.ncbi.nlm.nih.gov/pubmed/29443937 http://dx.doi.org/10.3390/insects9010024 |
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author | Ouessou Idrissou, Franck Huang, Qiang Yañez, Orlando Akinwande, Kayode Lawrence Neumann, Peter |
author_facet | Ouessou Idrissou, Franck Huang, Qiang Yañez, Orlando Akinwande, Kayode Lawrence Neumann, Peter |
author_sort | Ouessou Idrissou, Franck |
collection | PubMed |
description | Small hive beetles (SHBs), Aethina tumida, are parasites of social bee colonies native to sub-Saharan Africa and have become an invasive species at a global scale. Reliable Polymerase Chain Reaction (PCR) diagnosis of this mandatory pest is required to limit its further spread and impact. Here, we have developed SHB primers, which amplify for 10 native African locations and 10 reported introductions, but not for three closely related species (Aethina concolor, Aethina flavicollis, and Aethina inconspicua). We also show that adult honey bee workers can be used as matrices for PCR-based detection of SHBs. The sensitivity of this novel method appears to be 100%, which is identical to conventional visual screenings. Furthermore, the specificity of this novel approach was also high (90.91%). Since both sensitivity and specificity are high, we recommend this novel PCR method and the new primers for routine surveillance of hives in high-risk areas. |
format | Online Article Text |
id | pubmed-5872289 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-58722892018-03-29 PCR Diagnosis of Small Hive Beetles Ouessou Idrissou, Franck Huang, Qiang Yañez, Orlando Akinwande, Kayode Lawrence Neumann, Peter Insects Article Small hive beetles (SHBs), Aethina tumida, are parasites of social bee colonies native to sub-Saharan Africa and have become an invasive species at a global scale. Reliable Polymerase Chain Reaction (PCR) diagnosis of this mandatory pest is required to limit its further spread and impact. Here, we have developed SHB primers, which amplify for 10 native African locations and 10 reported introductions, but not for three closely related species (Aethina concolor, Aethina flavicollis, and Aethina inconspicua). We also show that adult honey bee workers can be used as matrices for PCR-based detection of SHBs. The sensitivity of this novel method appears to be 100%, which is identical to conventional visual screenings. Furthermore, the specificity of this novel approach was also high (90.91%). Since both sensitivity and specificity are high, we recommend this novel PCR method and the new primers for routine surveillance of hives in high-risk areas. MDPI 2018-02-14 /pmc/articles/PMC5872289/ /pubmed/29443937 http://dx.doi.org/10.3390/insects9010024 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Ouessou Idrissou, Franck Huang, Qiang Yañez, Orlando Akinwande, Kayode Lawrence Neumann, Peter PCR Diagnosis of Small Hive Beetles |
title | PCR Diagnosis of Small Hive Beetles |
title_full | PCR Diagnosis of Small Hive Beetles |
title_fullStr | PCR Diagnosis of Small Hive Beetles |
title_full_unstemmed | PCR Diagnosis of Small Hive Beetles |
title_short | PCR Diagnosis of Small Hive Beetles |
title_sort | pcr diagnosis of small hive beetles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5872289/ https://www.ncbi.nlm.nih.gov/pubmed/29443937 http://dx.doi.org/10.3390/insects9010024 |
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