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PCR Diagnosis of Small Hive Beetles

Small hive beetles (SHBs), Aethina tumida, are parasites of social bee colonies native to sub-Saharan Africa and have become an invasive species at a global scale. Reliable Polymerase Chain Reaction (PCR) diagnosis of this mandatory pest is required to limit its further spread and impact. Here, we h...

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Detalles Bibliográficos
Autores principales: Ouessou Idrissou, Franck, Huang, Qiang, Yañez, Orlando, Akinwande, Kayode Lawrence, Neumann, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5872289/
https://www.ncbi.nlm.nih.gov/pubmed/29443937
http://dx.doi.org/10.3390/insects9010024
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author Ouessou Idrissou, Franck
Huang, Qiang
Yañez, Orlando
Akinwande, Kayode Lawrence
Neumann, Peter
author_facet Ouessou Idrissou, Franck
Huang, Qiang
Yañez, Orlando
Akinwande, Kayode Lawrence
Neumann, Peter
author_sort Ouessou Idrissou, Franck
collection PubMed
description Small hive beetles (SHBs), Aethina tumida, are parasites of social bee colonies native to sub-Saharan Africa and have become an invasive species at a global scale. Reliable Polymerase Chain Reaction (PCR) diagnosis of this mandatory pest is required to limit its further spread and impact. Here, we have developed SHB primers, which amplify for 10 native African locations and 10 reported introductions, but not for three closely related species (Aethina concolor, Aethina flavicollis, and Aethina inconspicua). We also show that adult honey bee workers can be used as matrices for PCR-based detection of SHBs. The sensitivity of this novel method appears to be 100%, which is identical to conventional visual screenings. Furthermore, the specificity of this novel approach was also high (90.91%). Since both sensitivity and specificity are high, we recommend this novel PCR method and the new primers for routine surveillance of hives in high-risk areas.
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spelling pubmed-58722892018-03-29 PCR Diagnosis of Small Hive Beetles Ouessou Idrissou, Franck Huang, Qiang Yañez, Orlando Akinwande, Kayode Lawrence Neumann, Peter Insects Article Small hive beetles (SHBs), Aethina tumida, are parasites of social bee colonies native to sub-Saharan Africa and have become an invasive species at a global scale. Reliable Polymerase Chain Reaction (PCR) diagnosis of this mandatory pest is required to limit its further spread and impact. Here, we have developed SHB primers, which amplify for 10 native African locations and 10 reported introductions, but not for three closely related species (Aethina concolor, Aethina flavicollis, and Aethina inconspicua). We also show that adult honey bee workers can be used as matrices for PCR-based detection of SHBs. The sensitivity of this novel method appears to be 100%, which is identical to conventional visual screenings. Furthermore, the specificity of this novel approach was also high (90.91%). Since both sensitivity and specificity are high, we recommend this novel PCR method and the new primers for routine surveillance of hives in high-risk areas. MDPI 2018-02-14 /pmc/articles/PMC5872289/ /pubmed/29443937 http://dx.doi.org/10.3390/insects9010024 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ouessou Idrissou, Franck
Huang, Qiang
Yañez, Orlando
Akinwande, Kayode Lawrence
Neumann, Peter
PCR Diagnosis of Small Hive Beetles
title PCR Diagnosis of Small Hive Beetles
title_full PCR Diagnosis of Small Hive Beetles
title_fullStr PCR Diagnosis of Small Hive Beetles
title_full_unstemmed PCR Diagnosis of Small Hive Beetles
title_short PCR Diagnosis of Small Hive Beetles
title_sort pcr diagnosis of small hive beetles
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5872289/
https://www.ncbi.nlm.nih.gov/pubmed/29443937
http://dx.doi.org/10.3390/insects9010024
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