Cargando…
Cross-Resistance: A Consequence of Bi-partite Host-Parasite Coevolution
Host-parasite coevolution can influence interactions of the host and parasite with the wider ecological community. One way that this may manifest is in cross-resistance towards other parasites, which has been observed to occur in some host-parasite evolution experiments. In this paper, we test for c...
Autores principales: | , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5872293/ https://www.ncbi.nlm.nih.gov/pubmed/29495405 http://dx.doi.org/10.3390/insects9010028 |
_version_ | 1783309805449904128 |
---|---|
author | Biswas, Tilottama Joop, Gerrit Rafaluk-Mohr, Charlotte |
author_facet | Biswas, Tilottama Joop, Gerrit Rafaluk-Mohr, Charlotte |
author_sort | Biswas, Tilottama |
collection | PubMed |
description | Host-parasite coevolution can influence interactions of the host and parasite with the wider ecological community. One way that this may manifest is in cross-resistance towards other parasites, which has been observed to occur in some host-parasite evolution experiments. In this paper, we test for cross-resistance towards Bacillus thuringiensis and Pseudomonas entomophila in the red flour beetle Tribolium castaneum, which was previously allowed to coevolve with the generalist entomopathogenic fungus Beauveria bassiana. We combine survival and gene expression assays upon infection to test for cross-resistance and underlying mechanisms. We show that larvae of T. castaneum that evolved with B. bassiana under coevolutionary conditions were positively cross-resistant to the bacterium B. thuringiensis, but not P. entomophila. Positive cross-resistance was mirrored at the gene expression level with markers that were representative of the oral route of infection being upregulated upon B. bassiana exposure. We find that positive cross-resistance towards B. thuringiensis evolved in T. castaneum as a consequence of its coevolutionary interactions with B. bassiana. This cross-resistance appears to be a consequence of resistance to oral toxicity. The fact that coevolution with B. bassiana results in resistance to B. thuringiensis, but not P. entomophila implies that B. thuringiensis and B. bassiana may share mechanisms of infection or toxicity not shared by P. entomophila. This supports previous suggestions that B. bassiana may possess Cry-like toxins, similar to those found in B. thuringiensis, which allow it to infect orally. |
format | Online Article Text |
id | pubmed-5872293 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-58722932018-03-29 Cross-Resistance: A Consequence of Bi-partite Host-Parasite Coevolution Biswas, Tilottama Joop, Gerrit Rafaluk-Mohr, Charlotte Insects Article Host-parasite coevolution can influence interactions of the host and parasite with the wider ecological community. One way that this may manifest is in cross-resistance towards other parasites, which has been observed to occur in some host-parasite evolution experiments. In this paper, we test for cross-resistance towards Bacillus thuringiensis and Pseudomonas entomophila in the red flour beetle Tribolium castaneum, which was previously allowed to coevolve with the generalist entomopathogenic fungus Beauveria bassiana. We combine survival and gene expression assays upon infection to test for cross-resistance and underlying mechanisms. We show that larvae of T. castaneum that evolved with B. bassiana under coevolutionary conditions were positively cross-resistant to the bacterium B. thuringiensis, but not P. entomophila. Positive cross-resistance was mirrored at the gene expression level with markers that were representative of the oral route of infection being upregulated upon B. bassiana exposure. We find that positive cross-resistance towards B. thuringiensis evolved in T. castaneum as a consequence of its coevolutionary interactions with B. bassiana. This cross-resistance appears to be a consequence of resistance to oral toxicity. The fact that coevolution with B. bassiana results in resistance to B. thuringiensis, but not P. entomophila implies that B. thuringiensis and B. bassiana may share mechanisms of infection or toxicity not shared by P. entomophila. This supports previous suggestions that B. bassiana may possess Cry-like toxins, similar to those found in B. thuringiensis, which allow it to infect orally. MDPI 2018-02-26 /pmc/articles/PMC5872293/ /pubmed/29495405 http://dx.doi.org/10.3390/insects9010028 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Biswas, Tilottama Joop, Gerrit Rafaluk-Mohr, Charlotte Cross-Resistance: A Consequence of Bi-partite Host-Parasite Coevolution |
title | Cross-Resistance: A Consequence of Bi-partite Host-Parasite Coevolution |
title_full | Cross-Resistance: A Consequence of Bi-partite Host-Parasite Coevolution |
title_fullStr | Cross-Resistance: A Consequence of Bi-partite Host-Parasite Coevolution |
title_full_unstemmed | Cross-Resistance: A Consequence of Bi-partite Host-Parasite Coevolution |
title_short | Cross-Resistance: A Consequence of Bi-partite Host-Parasite Coevolution |
title_sort | cross-resistance: a consequence of bi-partite host-parasite coevolution |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5872293/ https://www.ncbi.nlm.nih.gov/pubmed/29495405 http://dx.doi.org/10.3390/insects9010028 |
work_keys_str_mv | AT biswastilottama crossresistanceaconsequenceofbipartitehostparasitecoevolution AT joopgerrit crossresistanceaconsequenceofbipartitehostparasitecoevolution AT rafalukmohrcharlotte crossresistanceaconsequenceofbipartitehostparasitecoevolution |