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Biofilm Formation and Resistance to Fungicides in Clinically Relevant Members of the Fungal Genus Fusarium

Clinically relevant members of the fungal genus, Fusarium, exhibit an extraordinary genetic diversity and cause a wide spectrum of infections in both healthy individuals and immunocompromised patients. Generally, Fusarium species are intrinsically resistant to all systemic antifungals. We investigat...

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Autores principales: Sav, Hafize, Rafati, Haleh, Öz, Yasemin, Dalyan-Cilo, Burcu, Ener, Beyza, Mohammadi, Faezeh, Ilkit, Macit, van Diepeningen, Anne D., Seyedmousavi, Seyedmojtaba
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5872319/
https://www.ncbi.nlm.nih.gov/pubmed/29371509
http://dx.doi.org/10.3390/jof4010016
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author Sav, Hafize
Rafati, Haleh
Öz, Yasemin
Dalyan-Cilo, Burcu
Ener, Beyza
Mohammadi, Faezeh
Ilkit, Macit
van Diepeningen, Anne D.
Seyedmousavi, Seyedmojtaba
author_facet Sav, Hafize
Rafati, Haleh
Öz, Yasemin
Dalyan-Cilo, Burcu
Ener, Beyza
Mohammadi, Faezeh
Ilkit, Macit
van Diepeningen, Anne D.
Seyedmousavi, Seyedmojtaba
author_sort Sav, Hafize
collection PubMed
description Clinically relevant members of the fungal genus, Fusarium, exhibit an extraordinary genetic diversity and cause a wide spectrum of infections in both healthy individuals and immunocompromised patients. Generally, Fusarium species are intrinsically resistant to all systemic antifungals. We investigated whether the presence or absence of the ability to produce biofilms across and within Fusarium species complexes is linked to higher resistance against antifungals. A collection of 41 Fusarium strains, obtained from 38 patients with superficial and systemic infections, and three infected crops, were tested, including 25 species within the Fusarium fujikuroi species complex, 14 from the Fusarium solani species complex (FSSC), one Fusarium dimerum species complex, and one Fusarium oxysporum species complex isolate. Of all isolates tested, only seven strains from two species of FSSC, five F. petroliphilum and two F. keratoplasticum strains, recovered from blood, nail scrapings, and nasal biopsy samples, could produce biofilms under the tested conditions. In the liquid culture tested, sessile biofilm-forming Fusarium strains exhibited elevated minimum inhibitory concentrations (MICs) for amphotericin B, voriconazole, and posaconazole, compared to their planktonic counterparts, indicating that the ability to form biofilm may significantly increase resistance. Collectively, this suggests that once a surface adherent biofilm has been established, therapies designed to kill planktonic cells of Fusarium are ineffective.
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spelling pubmed-58723192018-03-30 Biofilm Formation and Resistance to Fungicides in Clinically Relevant Members of the Fungal Genus Fusarium Sav, Hafize Rafati, Haleh Öz, Yasemin Dalyan-Cilo, Burcu Ener, Beyza Mohammadi, Faezeh Ilkit, Macit van Diepeningen, Anne D. Seyedmousavi, Seyedmojtaba J Fungi (Basel) Article Clinically relevant members of the fungal genus, Fusarium, exhibit an extraordinary genetic diversity and cause a wide spectrum of infections in both healthy individuals and immunocompromised patients. Generally, Fusarium species are intrinsically resistant to all systemic antifungals. We investigated whether the presence or absence of the ability to produce biofilms across and within Fusarium species complexes is linked to higher resistance against antifungals. A collection of 41 Fusarium strains, obtained from 38 patients with superficial and systemic infections, and three infected crops, were tested, including 25 species within the Fusarium fujikuroi species complex, 14 from the Fusarium solani species complex (FSSC), one Fusarium dimerum species complex, and one Fusarium oxysporum species complex isolate. Of all isolates tested, only seven strains from two species of FSSC, five F. petroliphilum and two F. keratoplasticum strains, recovered from blood, nail scrapings, and nasal biopsy samples, could produce biofilms under the tested conditions. In the liquid culture tested, sessile biofilm-forming Fusarium strains exhibited elevated minimum inhibitory concentrations (MICs) for amphotericin B, voriconazole, and posaconazole, compared to their planktonic counterparts, indicating that the ability to form biofilm may significantly increase resistance. Collectively, this suggests that once a surface adherent biofilm has been established, therapies designed to kill planktonic cells of Fusarium are ineffective. MDPI 2018-01-23 /pmc/articles/PMC5872319/ /pubmed/29371509 http://dx.doi.org/10.3390/jof4010016 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Sav, Hafize
Rafati, Haleh
Öz, Yasemin
Dalyan-Cilo, Burcu
Ener, Beyza
Mohammadi, Faezeh
Ilkit, Macit
van Diepeningen, Anne D.
Seyedmousavi, Seyedmojtaba
Biofilm Formation and Resistance to Fungicides in Clinically Relevant Members of the Fungal Genus Fusarium
title Biofilm Formation and Resistance to Fungicides in Clinically Relevant Members of the Fungal Genus Fusarium
title_full Biofilm Formation and Resistance to Fungicides in Clinically Relevant Members of the Fungal Genus Fusarium
title_fullStr Biofilm Formation and Resistance to Fungicides in Clinically Relevant Members of the Fungal Genus Fusarium
title_full_unstemmed Biofilm Formation and Resistance to Fungicides in Clinically Relevant Members of the Fungal Genus Fusarium
title_short Biofilm Formation and Resistance to Fungicides in Clinically Relevant Members of the Fungal Genus Fusarium
title_sort biofilm formation and resistance to fungicides in clinically relevant members of the fungal genus fusarium
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5872319/
https://www.ncbi.nlm.nih.gov/pubmed/29371509
http://dx.doi.org/10.3390/jof4010016
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