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Inner Leaf Gel of Aloe striata Induces Adhesion-Reducing Morphological Hyphal Aberrations

Fungi, particularly molds that are cosmopolitan in soils, are frequent etiologic agents of opportunistic mycoses. Members of the Fusarium solani and Fusarium oxysporum species complexes are the most commonly implicated etiologic agents of opportunistic fusarial infections in mammals, while Paecilomy...

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Autores principales: Wada, Gloria, Vincent, Michael, Lee, Marcia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5872326/
https://www.ncbi.nlm.nih.gov/pubmed/29419783
http://dx.doi.org/10.3390/jof4010023
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author Wada, Gloria
Vincent, Michael
Lee, Marcia
author_facet Wada, Gloria
Vincent, Michael
Lee, Marcia
author_sort Wada, Gloria
collection PubMed
description Fungi, particularly molds that are cosmopolitan in soils, are frequent etiologic agents of opportunistic mycoses. Members of the Fusarium solani and Fusarium oxysporum species complexes are the most commonly implicated etiologic agents of opportunistic fusarial infections in mammals, while Paecilomyces variotii is one of the most frequently encountered Paecilomyces species in human infections. Prevention and treatment of these mycoses are problematic because available antimycotics are limited and often have toxic side effects. Popular folk medicines, such as the inner leaf gel from Aloe spp., offer potential sources for novel antimycotic compounds. To screen for antifungal properties of Aloe striata, we treated conidia of three strains each of F. solani, F. oxysporum, and P. variotii with homogenized and filtered inner leaf gel. Exposure to gel homogenates caused minimal inhibition of conidial germination in tested strains. However, it significantly increased the frequency of hyphal aberrations characterized by increased hyphal diameters that resulted in intervals of non-parallel cell walls. Non-parallel cell walls ostensibly reduce total hyphal surface area available for adhesion. We found a significant decrease in the ability of aberrated P. variotii hyphae to remain adhered to microscope slides after repeated washing with reverse osmosis water. Our results suggest that treatment with A. striata contributes to a decrease in the adhesion frequency of tested P. variotii strains.
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spelling pubmed-58723262018-03-30 Inner Leaf Gel of Aloe striata Induces Adhesion-Reducing Morphological Hyphal Aberrations Wada, Gloria Vincent, Michael Lee, Marcia J Fungi (Basel) Article Fungi, particularly molds that are cosmopolitan in soils, are frequent etiologic agents of opportunistic mycoses. Members of the Fusarium solani and Fusarium oxysporum species complexes are the most commonly implicated etiologic agents of opportunistic fusarial infections in mammals, while Paecilomyces variotii is one of the most frequently encountered Paecilomyces species in human infections. Prevention and treatment of these mycoses are problematic because available antimycotics are limited and often have toxic side effects. Popular folk medicines, such as the inner leaf gel from Aloe spp., offer potential sources for novel antimycotic compounds. To screen for antifungal properties of Aloe striata, we treated conidia of three strains each of F. solani, F. oxysporum, and P. variotii with homogenized and filtered inner leaf gel. Exposure to gel homogenates caused minimal inhibition of conidial germination in tested strains. However, it significantly increased the frequency of hyphal aberrations characterized by increased hyphal diameters that resulted in intervals of non-parallel cell walls. Non-parallel cell walls ostensibly reduce total hyphal surface area available for adhesion. We found a significant decrease in the ability of aberrated P. variotii hyphae to remain adhered to microscope slides after repeated washing with reverse osmosis water. Our results suggest that treatment with A. striata contributes to a decrease in the adhesion frequency of tested P. variotii strains. MDPI 2018-02-08 /pmc/articles/PMC5872326/ /pubmed/29419783 http://dx.doi.org/10.3390/jof4010023 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wada, Gloria
Vincent, Michael
Lee, Marcia
Inner Leaf Gel of Aloe striata Induces Adhesion-Reducing Morphological Hyphal Aberrations
title Inner Leaf Gel of Aloe striata Induces Adhesion-Reducing Morphological Hyphal Aberrations
title_full Inner Leaf Gel of Aloe striata Induces Adhesion-Reducing Morphological Hyphal Aberrations
title_fullStr Inner Leaf Gel of Aloe striata Induces Adhesion-Reducing Morphological Hyphal Aberrations
title_full_unstemmed Inner Leaf Gel of Aloe striata Induces Adhesion-Reducing Morphological Hyphal Aberrations
title_short Inner Leaf Gel of Aloe striata Induces Adhesion-Reducing Morphological Hyphal Aberrations
title_sort inner leaf gel of aloe striata induces adhesion-reducing morphological hyphal aberrations
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5872326/
https://www.ncbi.nlm.nih.gov/pubmed/29419783
http://dx.doi.org/10.3390/jof4010023
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