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Undifferentiated Adipose Tissue Stem Cell Transplantation Promotes Hepatic Regeneration, Ameliorates Histopathologic Damage of the Liver, and Upregulates the Expression of Liver Regeneration- and Liver-Specific Genes in a Rat Model of Partial Hepatectomy

OBJECTIVE: Adipose tissue stem cells (ADSCs) present a promising therapeutic method to alleviate liver failure (LF). The purpose of this prospective study was to evaluate the efficacy of undifferentiated ADSC transplantation on liver regeneration and on the expression of liver regeneration- and live...

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Autores principales: Apostolou, Konstantinos G., Papanikolaou, Ioannis G., Katselis, Charalampos, Feretis, Themistoklis, Kletsas, Dimitrios, Konstadoulakis, Manousos M., Lymperi, Maria, Saetta, Angelica A., Tsikalakis, Spiros, Agrogiannis, George, Patsouris, Efstratios, Zografos, George C., Papalois, Apostolos E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5872619/
https://www.ncbi.nlm.nih.gov/pubmed/29731771
http://dx.doi.org/10.1155/2018/1393607
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author Apostolou, Konstantinos G.
Papanikolaou, Ioannis G.
Katselis, Charalampos
Feretis, Themistoklis
Kletsas, Dimitrios
Konstadoulakis, Manousos M.
Lymperi, Maria
Saetta, Angelica A.
Tsikalakis, Spiros
Agrogiannis, George
Patsouris, Efstratios
Zografos, George C.
Papalois, Apostolos E.
author_facet Apostolou, Konstantinos G.
Papanikolaou, Ioannis G.
Katselis, Charalampos
Feretis, Themistoklis
Kletsas, Dimitrios
Konstadoulakis, Manousos M.
Lymperi, Maria
Saetta, Angelica A.
Tsikalakis, Spiros
Agrogiannis, George
Patsouris, Efstratios
Zografos, George C.
Papalois, Apostolos E.
author_sort Apostolou, Konstantinos G.
collection PubMed
description OBJECTIVE: Adipose tissue stem cells (ADSCs) present a promising therapeutic method to alleviate liver failure (LF). The purpose of this prospective study was to evaluate the efficacy of undifferentiated ADSC transplantation on liver regeneration and on the expression of liver regeneration- and liver-specific genes, following 60% partial hepatectomy (PHx). METHODS: Sixty female rats were subjected to PHx and were transplanted with 10(6) or 2 × 10(6) ADSCs, either into the portal vein (PV) or into the hepatic parenchyma. Animals of the control group were not transplanted and served as controls. Animals were sacrificed on the 4th, the 7th, or the 15th postoperative day (POD). RESULTS: The transplanted ADSCs were successfully engrafted into the liver parenchyma and ameliorated the histopathologic damage on the 7th and 15th POD. All transplanted animals demonstrated a significantly higher liver regeneration rate on the 4th and 7th POD, compared with the control group. The expression of hepatocyte growth factor, α-fetoprotein, tyrosine aminotransferase, hepatocyte nuclear factor 4a, and cytochrome P450 1A2 was significantly upregulated, compared with the control group. CONCLUSIONS: Although undifferentiated, ADSC transplantation significantly enhanced the liver regeneration process. These findings may be proven clinically valuable, especially in cases of acute LF.
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spelling pubmed-58726192018-05-06 Undifferentiated Adipose Tissue Stem Cell Transplantation Promotes Hepatic Regeneration, Ameliorates Histopathologic Damage of the Liver, and Upregulates the Expression of Liver Regeneration- and Liver-Specific Genes in a Rat Model of Partial Hepatectomy Apostolou, Konstantinos G. Papanikolaou, Ioannis G. Katselis, Charalampos Feretis, Themistoklis Kletsas, Dimitrios Konstadoulakis, Manousos M. Lymperi, Maria Saetta, Angelica A. Tsikalakis, Spiros Agrogiannis, George Patsouris, Efstratios Zografos, George C. Papalois, Apostolos E. Stem Cells Int Research Article OBJECTIVE: Adipose tissue stem cells (ADSCs) present a promising therapeutic method to alleviate liver failure (LF). The purpose of this prospective study was to evaluate the efficacy of undifferentiated ADSC transplantation on liver regeneration and on the expression of liver regeneration- and liver-specific genes, following 60% partial hepatectomy (PHx). METHODS: Sixty female rats were subjected to PHx and were transplanted with 10(6) or 2 × 10(6) ADSCs, either into the portal vein (PV) or into the hepatic parenchyma. Animals of the control group were not transplanted and served as controls. Animals were sacrificed on the 4th, the 7th, or the 15th postoperative day (POD). RESULTS: The transplanted ADSCs were successfully engrafted into the liver parenchyma and ameliorated the histopathologic damage on the 7th and 15th POD. All transplanted animals demonstrated a significantly higher liver regeneration rate on the 4th and 7th POD, compared with the control group. The expression of hepatocyte growth factor, α-fetoprotein, tyrosine aminotransferase, hepatocyte nuclear factor 4a, and cytochrome P450 1A2 was significantly upregulated, compared with the control group. CONCLUSIONS: Although undifferentiated, ADSC transplantation significantly enhanced the liver regeneration process. These findings may be proven clinically valuable, especially in cases of acute LF. Hindawi 2018-03-14 /pmc/articles/PMC5872619/ /pubmed/29731771 http://dx.doi.org/10.1155/2018/1393607 Text en Copyright © 2018 Konstantinos G. Apostolou et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Apostolou, Konstantinos G.
Papanikolaou, Ioannis G.
Katselis, Charalampos
Feretis, Themistoklis
Kletsas, Dimitrios
Konstadoulakis, Manousos M.
Lymperi, Maria
Saetta, Angelica A.
Tsikalakis, Spiros
Agrogiannis, George
Patsouris, Efstratios
Zografos, George C.
Papalois, Apostolos E.
Undifferentiated Adipose Tissue Stem Cell Transplantation Promotes Hepatic Regeneration, Ameliorates Histopathologic Damage of the Liver, and Upregulates the Expression of Liver Regeneration- and Liver-Specific Genes in a Rat Model of Partial Hepatectomy
title Undifferentiated Adipose Tissue Stem Cell Transplantation Promotes Hepatic Regeneration, Ameliorates Histopathologic Damage of the Liver, and Upregulates the Expression of Liver Regeneration- and Liver-Specific Genes in a Rat Model of Partial Hepatectomy
title_full Undifferentiated Adipose Tissue Stem Cell Transplantation Promotes Hepatic Regeneration, Ameliorates Histopathologic Damage of the Liver, and Upregulates the Expression of Liver Regeneration- and Liver-Specific Genes in a Rat Model of Partial Hepatectomy
title_fullStr Undifferentiated Adipose Tissue Stem Cell Transplantation Promotes Hepatic Regeneration, Ameliorates Histopathologic Damage of the Liver, and Upregulates the Expression of Liver Regeneration- and Liver-Specific Genes in a Rat Model of Partial Hepatectomy
title_full_unstemmed Undifferentiated Adipose Tissue Stem Cell Transplantation Promotes Hepatic Regeneration, Ameliorates Histopathologic Damage of the Liver, and Upregulates the Expression of Liver Regeneration- and Liver-Specific Genes in a Rat Model of Partial Hepatectomy
title_short Undifferentiated Adipose Tissue Stem Cell Transplantation Promotes Hepatic Regeneration, Ameliorates Histopathologic Damage of the Liver, and Upregulates the Expression of Liver Regeneration- and Liver-Specific Genes in a Rat Model of Partial Hepatectomy
title_sort undifferentiated adipose tissue stem cell transplantation promotes hepatic regeneration, ameliorates histopathologic damage of the liver, and upregulates the expression of liver regeneration- and liver-specific genes in a rat model of partial hepatectomy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5872619/
https://www.ncbi.nlm.nih.gov/pubmed/29731771
http://dx.doi.org/10.1155/2018/1393607
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