Growth plate expression profiling: Large and small breed dogs provide new insights in endochondral bone formation
The difference in the adult height of mammals, and hence in endochondral bone formation, is not yet fully understood and may serve to identify targets for bone and cartilage regeneration. In line with this hypothesis, the intra‐species disparity between the adult height of Great Danes and Miniature...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5873274/ https://www.ncbi.nlm.nih.gov/pubmed/28681971 http://dx.doi.org/10.1002/jor.23647 |
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author | Teunissen, Michelle Riemers, Frank M. van Leenen, Dik Groot Koerkamp, Marian J. A. Meij, Björn P. Alblas, Jacqueline Penning, Louis C. Miranda‐Bedate, Alberto Tryfonidou, Marianna A. |
author_facet | Teunissen, Michelle Riemers, Frank M. van Leenen, Dik Groot Koerkamp, Marian J. A. Meij, Björn P. Alblas, Jacqueline Penning, Louis C. Miranda‐Bedate, Alberto Tryfonidou, Marianna A. |
author_sort | Teunissen, Michelle |
collection | PubMed |
description | The difference in the adult height of mammals, and hence in endochondral bone formation, is not yet fully understood and may serve to identify targets for bone and cartilage regeneration. In line with this hypothesis, the intra‐species disparity between the adult height of Great Danes and Miniature Poodles was investigated at a transcriptional level. Microarray analysis of the growth plate of five Great Danes and five Miniature Poodles revealed 2,981 unique genes that were differentially expressed, including many genes with an unknown role in skeletal development. A signaling pathway impact analysis indicated activation of the cell cycle, extracellular matrix receptor interaction and the tight junction pathway, and inhibition of pathways associated with inflammation and the complement cascade. In additional validation steps, the gene expression profile of the separate growth plate zones for both dog breeds were determined. Given that the BMP signaling is known for its crucial role in skeletal development and fracture healing, and BMP‐2 is used in orthopaedic and spine procedures for bone augmentation, further investigations concentrated on the BMP pathway.The canonical BMP‐2 and BMP‐6 signaling pathway was activated in the Great Danes compared to Miniature Poodles. In conclusion, investigating the differential expression of genes involved in endochondral bone formation in small and large breed dogs, could be a game changing strategy to provide new insights in growth plate development and identify new targets for bone and cartilage regeneration. © 2017 The Authors. Journal of Orthopaedic Research® published by Wiley Periodicals, Inc. on behalf of the Orthopaedic Research Society. J Orthop Res 36:138–148, 2018. |
format | Online Article Text |
id | pubmed-5873274 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2017 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-58732742018-03-31 Growth plate expression profiling: Large and small breed dogs provide new insights in endochondral bone formation Teunissen, Michelle Riemers, Frank M. van Leenen, Dik Groot Koerkamp, Marian J. A. Meij, Björn P. Alblas, Jacqueline Penning, Louis C. Miranda‐Bedate, Alberto Tryfonidou, Marianna A. J Orthop Res Research Articles The difference in the adult height of mammals, and hence in endochondral bone formation, is not yet fully understood and may serve to identify targets for bone and cartilage regeneration. In line with this hypothesis, the intra‐species disparity between the adult height of Great Danes and Miniature Poodles was investigated at a transcriptional level. Microarray analysis of the growth plate of five Great Danes and five Miniature Poodles revealed 2,981 unique genes that were differentially expressed, including many genes with an unknown role in skeletal development. A signaling pathway impact analysis indicated activation of the cell cycle, extracellular matrix receptor interaction and the tight junction pathway, and inhibition of pathways associated with inflammation and the complement cascade. In additional validation steps, the gene expression profile of the separate growth plate zones for both dog breeds were determined. Given that the BMP signaling is known for its crucial role in skeletal development and fracture healing, and BMP‐2 is used in orthopaedic and spine procedures for bone augmentation, further investigations concentrated on the BMP pathway.The canonical BMP‐2 and BMP‐6 signaling pathway was activated in the Great Danes compared to Miniature Poodles. In conclusion, investigating the differential expression of genes involved in endochondral bone formation in small and large breed dogs, could be a game changing strategy to provide new insights in growth plate development and identify new targets for bone and cartilage regeneration. © 2017 The Authors. Journal of Orthopaedic Research® published by Wiley Periodicals, Inc. on behalf of the Orthopaedic Research Society. J Orthop Res 36:138–148, 2018. John Wiley and Sons Inc. 2017-08-14 2018-01 /pmc/articles/PMC5873274/ /pubmed/28681971 http://dx.doi.org/10.1002/jor.23647 Text en © 2017 The Authors. Journal of Orthopaedic Research® Published by Wiley Periodicals Inc. on behalf of the Orthopaedic Research Society. This is an open access article under the terms of the Creative Commons Attribution‐NonCommercial‐NoDerivs (http://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Teunissen, Michelle Riemers, Frank M. van Leenen, Dik Groot Koerkamp, Marian J. A. Meij, Björn P. Alblas, Jacqueline Penning, Louis C. Miranda‐Bedate, Alberto Tryfonidou, Marianna A. Growth plate expression profiling: Large and small breed dogs provide new insights in endochondral bone formation |
title | Growth plate expression profiling: Large and small breed dogs provide new insights in endochondral bone formation |
title_full | Growth plate expression profiling: Large and small breed dogs provide new insights in endochondral bone formation |
title_fullStr | Growth plate expression profiling: Large and small breed dogs provide new insights in endochondral bone formation |
title_full_unstemmed | Growth plate expression profiling: Large and small breed dogs provide new insights in endochondral bone formation |
title_short | Growth plate expression profiling: Large and small breed dogs provide new insights in endochondral bone formation |
title_sort | growth plate expression profiling: large and small breed dogs provide new insights in endochondral bone formation |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5873274/ https://www.ncbi.nlm.nih.gov/pubmed/28681971 http://dx.doi.org/10.1002/jor.23647 |
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