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Electrochemical Detection of Dopamine via Assisted Ion Transfer at Nanopipet Electrode Using Cyclic Voltammetry

We present here the detection of dopamine (DA) at nanopipet electrodes with radii of hundreds of nanometers ranging from 160 nm to 480 nm. Dibenzo-18-crown-6 (DB18C6) was employed as an ionophore to facilitate DA transfer, resulting in a half-wave transfer potential, E(1/2, DA), of −0.322 (±0.020) V...

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Autores principales: Colombo, Michelle L., McNeil, Swami, Iwai, Nicholas, Chang, Albert, Shen, Mei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5873601/
https://www.ncbi.nlm.nih.gov/pubmed/29606735
http://dx.doi.org/10.1149/2.0091604jes
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author Colombo, Michelle L.
McNeil, Swami
Iwai, Nicholas
Chang, Albert
Shen, Mei
author_facet Colombo, Michelle L.
McNeil, Swami
Iwai, Nicholas
Chang, Albert
Shen, Mei
author_sort Colombo, Michelle L.
collection PubMed
description We present here the detection of dopamine (DA) at nanopipet electrodes with radii of hundreds of nanometers ranging from 160 nm to 480 nm. Dibenzo-18-crown-6 (DB18C6) was employed as an ionophore to facilitate DA transfer, resulting in a half-wave transfer potential, E(1/2, DA), of −0.322 (±0.020) V vs. E(1/2, TBA). Well-defined steady-state sigmoidal cyclic voltammograms were observed for the transfer of DA. High resolution scanning electron microscopy was used to measure the size and taper angle of the nanopipet electrodes. The detection is linear with concentration of DA ranging from 0.25 mM to 2 mM; calculated diffusion coefficient at nanopipet electrodes with above mentioned sizes is 4.87 (±0.28) × 10(−10) m(2)/s. The effect of the common interferent ascorbic acid on DA detection with nanopipet electrodes was evaluated, where DA detection still shows linear behavior with well-defined sigmoidal CVs with E(1/2, DA) being −0.328 (±0.029) V vs. E(1/2, TBA). The diffusion coefficient for DA transfer in MgCl(2) with the presence of 2 mM AA was measured to be 1.93 (±0.59) × 10(−10) m(2)/s on nanoelectrodes with radii from 161 nm to 263 nm, but the physiological concentration of 0.1 mM AA had no effect on DA’s diffusion coefficient.
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spelling pubmed-58736012018-03-28 Electrochemical Detection of Dopamine via Assisted Ion Transfer at Nanopipet Electrode Using Cyclic Voltammetry Colombo, Michelle L. McNeil, Swami Iwai, Nicholas Chang, Albert Shen, Mei J Electrochem Soc Article We present here the detection of dopamine (DA) at nanopipet electrodes with radii of hundreds of nanometers ranging from 160 nm to 480 nm. Dibenzo-18-crown-6 (DB18C6) was employed as an ionophore to facilitate DA transfer, resulting in a half-wave transfer potential, E(1/2, DA), of −0.322 (±0.020) V vs. E(1/2, TBA). Well-defined steady-state sigmoidal cyclic voltammograms were observed for the transfer of DA. High resolution scanning electron microscopy was used to measure the size and taper angle of the nanopipet electrodes. The detection is linear with concentration of DA ranging from 0.25 mM to 2 mM; calculated diffusion coefficient at nanopipet electrodes with above mentioned sizes is 4.87 (±0.28) × 10(−10) m(2)/s. The effect of the common interferent ascorbic acid on DA detection with nanopipet electrodes was evaluated, where DA detection still shows linear behavior with well-defined sigmoidal CVs with E(1/2, DA) being −0.328 (±0.029) V vs. E(1/2, TBA). The diffusion coefficient for DA transfer in MgCl(2) with the presence of 2 mM AA was measured to be 1.93 (±0.59) × 10(−10) m(2)/s on nanoelectrodes with radii from 161 nm to 263 nm, but the physiological concentration of 0.1 mM AA had no effect on DA’s diffusion coefficient. 2015-12-08 2016 /pmc/articles/PMC5873601/ /pubmed/29606735 http://dx.doi.org/10.1149/2.0091604jes Text en This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives 4.0 License (CC BY-NC-ND, http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is not changed in any way and is properly cited. For permission for commercial reuse, please email: oa@electrochem.org.
spellingShingle Article
Colombo, Michelle L.
McNeil, Swami
Iwai, Nicholas
Chang, Albert
Shen, Mei
Electrochemical Detection of Dopamine via Assisted Ion Transfer at Nanopipet Electrode Using Cyclic Voltammetry
title Electrochemical Detection of Dopamine via Assisted Ion Transfer at Nanopipet Electrode Using Cyclic Voltammetry
title_full Electrochemical Detection of Dopamine via Assisted Ion Transfer at Nanopipet Electrode Using Cyclic Voltammetry
title_fullStr Electrochemical Detection of Dopamine via Assisted Ion Transfer at Nanopipet Electrode Using Cyclic Voltammetry
title_full_unstemmed Electrochemical Detection of Dopamine via Assisted Ion Transfer at Nanopipet Electrode Using Cyclic Voltammetry
title_short Electrochemical Detection of Dopamine via Assisted Ion Transfer at Nanopipet Electrode Using Cyclic Voltammetry
title_sort electrochemical detection of dopamine via assisted ion transfer at nanopipet electrode using cyclic voltammetry
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5873601/
https://www.ncbi.nlm.nih.gov/pubmed/29606735
http://dx.doi.org/10.1149/2.0091604jes
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