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Electrochemical Detection of Dopamine via Assisted Ion Transfer at Nanopipet Electrode Using Cyclic Voltammetry
We present here the detection of dopamine (DA) at nanopipet electrodes with radii of hundreds of nanometers ranging from 160 nm to 480 nm. Dibenzo-18-crown-6 (DB18C6) was employed as an ionophore to facilitate DA transfer, resulting in a half-wave transfer potential, E(1/2, DA), of −0.322 (±0.020) V...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5873601/ https://www.ncbi.nlm.nih.gov/pubmed/29606735 http://dx.doi.org/10.1149/2.0091604jes |
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author | Colombo, Michelle L. McNeil, Swami Iwai, Nicholas Chang, Albert Shen, Mei |
author_facet | Colombo, Michelle L. McNeil, Swami Iwai, Nicholas Chang, Albert Shen, Mei |
author_sort | Colombo, Michelle L. |
collection | PubMed |
description | We present here the detection of dopamine (DA) at nanopipet electrodes with radii of hundreds of nanometers ranging from 160 nm to 480 nm. Dibenzo-18-crown-6 (DB18C6) was employed as an ionophore to facilitate DA transfer, resulting in a half-wave transfer potential, E(1/2, DA), of −0.322 (±0.020) V vs. E(1/2, TBA). Well-defined steady-state sigmoidal cyclic voltammograms were observed for the transfer of DA. High resolution scanning electron microscopy was used to measure the size and taper angle of the nanopipet electrodes. The detection is linear with concentration of DA ranging from 0.25 mM to 2 mM; calculated diffusion coefficient at nanopipet electrodes with above mentioned sizes is 4.87 (±0.28) × 10(−10) m(2)/s. The effect of the common interferent ascorbic acid on DA detection with nanopipet electrodes was evaluated, where DA detection still shows linear behavior with well-defined sigmoidal CVs with E(1/2, DA) being −0.328 (±0.029) V vs. E(1/2, TBA). The diffusion coefficient for DA transfer in MgCl(2) with the presence of 2 mM AA was measured to be 1.93 (±0.59) × 10(−10) m(2)/s on nanoelectrodes with radii from 161 nm to 263 nm, but the physiological concentration of 0.1 mM AA had no effect on DA’s diffusion coefficient. |
format | Online Article Text |
id | pubmed-5873601 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
record_format | MEDLINE/PubMed |
spelling | pubmed-58736012018-03-28 Electrochemical Detection of Dopamine via Assisted Ion Transfer at Nanopipet Electrode Using Cyclic Voltammetry Colombo, Michelle L. McNeil, Swami Iwai, Nicholas Chang, Albert Shen, Mei J Electrochem Soc Article We present here the detection of dopamine (DA) at nanopipet electrodes with radii of hundreds of nanometers ranging from 160 nm to 480 nm. Dibenzo-18-crown-6 (DB18C6) was employed as an ionophore to facilitate DA transfer, resulting in a half-wave transfer potential, E(1/2, DA), of −0.322 (±0.020) V vs. E(1/2, TBA). Well-defined steady-state sigmoidal cyclic voltammograms were observed for the transfer of DA. High resolution scanning electron microscopy was used to measure the size and taper angle of the nanopipet electrodes. The detection is linear with concentration of DA ranging from 0.25 mM to 2 mM; calculated diffusion coefficient at nanopipet electrodes with above mentioned sizes is 4.87 (±0.28) × 10(−10) m(2)/s. The effect of the common interferent ascorbic acid on DA detection with nanopipet electrodes was evaluated, where DA detection still shows linear behavior with well-defined sigmoidal CVs with E(1/2, DA) being −0.328 (±0.029) V vs. E(1/2, TBA). The diffusion coefficient for DA transfer in MgCl(2) with the presence of 2 mM AA was measured to be 1.93 (±0.59) × 10(−10) m(2)/s on nanoelectrodes with radii from 161 nm to 263 nm, but the physiological concentration of 0.1 mM AA had no effect on DA’s diffusion coefficient. 2015-12-08 2016 /pmc/articles/PMC5873601/ /pubmed/29606735 http://dx.doi.org/10.1149/2.0091604jes Text en This is an open access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives 4.0 License (CC BY-NC-ND, http://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reuse, distribution, and reproduction in any medium, provided the original work is not changed in any way and is properly cited. For permission for commercial reuse, please email: oa@electrochem.org. |
spellingShingle | Article Colombo, Michelle L. McNeil, Swami Iwai, Nicholas Chang, Albert Shen, Mei Electrochemical Detection of Dopamine via Assisted Ion Transfer at Nanopipet Electrode Using Cyclic Voltammetry |
title | Electrochemical Detection of Dopamine via Assisted Ion Transfer at Nanopipet Electrode Using Cyclic Voltammetry |
title_full | Electrochemical Detection of Dopamine via Assisted Ion Transfer at Nanopipet Electrode Using Cyclic Voltammetry |
title_fullStr | Electrochemical Detection of Dopamine via Assisted Ion Transfer at Nanopipet Electrode Using Cyclic Voltammetry |
title_full_unstemmed | Electrochemical Detection of Dopamine via Assisted Ion Transfer at Nanopipet Electrode Using Cyclic Voltammetry |
title_short | Electrochemical Detection of Dopamine via Assisted Ion Transfer at Nanopipet Electrode Using Cyclic Voltammetry |
title_sort | electrochemical detection of dopamine via assisted ion transfer at nanopipet electrode using cyclic voltammetry |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5873601/ https://www.ncbi.nlm.nih.gov/pubmed/29606735 http://dx.doi.org/10.1149/2.0091604jes |
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