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Osthole inhibits gastric cancer cell proliferation through regulation of PI3K/AKT

Osthole is an active compound isolated from Chinese herb Cnidium monnieri (L.) Cusson, and had been reported to possess antitumor effect. However, the effect of osthole on the gastric cancer cells has not been investigated. In this study, the effects of osthole on the proliferation of human gastric...

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Detalles Bibliográficos
Autores principales: Xu, Xiaojun, Liu, Xiaoyuan, Zhang, Yan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5873990/
https://www.ncbi.nlm.nih.gov/pubmed/29590128
http://dx.doi.org/10.1371/journal.pone.0193449
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author Xu, Xiaojun
Liu, Xiaoyuan
Zhang, Yan
author_facet Xu, Xiaojun
Liu, Xiaoyuan
Zhang, Yan
author_sort Xu, Xiaojun
collection PubMed
description Osthole is an active compound isolated from Chinese herb Cnidium monnieri (L.) Cusson, and had been reported to possess antitumor effect. However, the effect of osthole on the gastric cancer cells has not been investigated. In this study, the effects of osthole on the proliferation of human gastric cancer cells were tested. The data showed that osthole treatment significantly inhibited the proliferation of gastric cancer cells and resulted in the cell cycle arrest at G2/M phase in a dose-dependent manner. Western-blot study showed that the expression of cyclin B1 and cdc2 was markedly reduced by osthole. Moreover, expression of PI3K and pAKT was also significantly suppressed, and the results indicated that the inhibition of pAKT, cyclin B1, and cdc2 levels by osthole was notably enhanced by a PI3K inhibitor. These results demonstrate that osthole could inhibit gastric cancer cells proliferation via induction of cell cycle arrest at G2/M phase by the reduction of PI3K/AKT.
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spelling pubmed-58739902018-04-06 Osthole inhibits gastric cancer cell proliferation through regulation of PI3K/AKT Xu, Xiaojun Liu, Xiaoyuan Zhang, Yan PLoS One Research Article Osthole is an active compound isolated from Chinese herb Cnidium monnieri (L.) Cusson, and had been reported to possess antitumor effect. However, the effect of osthole on the gastric cancer cells has not been investigated. In this study, the effects of osthole on the proliferation of human gastric cancer cells were tested. The data showed that osthole treatment significantly inhibited the proliferation of gastric cancer cells and resulted in the cell cycle arrest at G2/M phase in a dose-dependent manner. Western-blot study showed that the expression of cyclin B1 and cdc2 was markedly reduced by osthole. Moreover, expression of PI3K and pAKT was also significantly suppressed, and the results indicated that the inhibition of pAKT, cyclin B1, and cdc2 levels by osthole was notably enhanced by a PI3K inhibitor. These results demonstrate that osthole could inhibit gastric cancer cells proliferation via induction of cell cycle arrest at G2/M phase by the reduction of PI3K/AKT. Public Library of Science 2018-03-28 /pmc/articles/PMC5873990/ /pubmed/29590128 http://dx.doi.org/10.1371/journal.pone.0193449 Text en © 2018 Xu et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Xu, Xiaojun
Liu, Xiaoyuan
Zhang, Yan
Osthole inhibits gastric cancer cell proliferation through regulation of PI3K/AKT
title Osthole inhibits gastric cancer cell proliferation through regulation of PI3K/AKT
title_full Osthole inhibits gastric cancer cell proliferation through regulation of PI3K/AKT
title_fullStr Osthole inhibits gastric cancer cell proliferation through regulation of PI3K/AKT
title_full_unstemmed Osthole inhibits gastric cancer cell proliferation through regulation of PI3K/AKT
title_short Osthole inhibits gastric cancer cell proliferation through regulation of PI3K/AKT
title_sort osthole inhibits gastric cancer cell proliferation through regulation of pi3k/akt
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5873990/
https://www.ncbi.nlm.nih.gov/pubmed/29590128
http://dx.doi.org/10.1371/journal.pone.0193449
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