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Soluble klotho as a marker of renal fibrosis and podocyte injuries in human kidneys

Klotho deficiency is relevant to renal fibrosis and podocyte injury in vivo and in vitro. We examined whether histological findings of renal biopsy specimens were associated with the levels of soluble klotho in humans. We investigated renal biopsy specimens of 67 patients and detailed microscopic fi...

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Autores principales: Cho, Nam-Jun, Han, Dong-Jae, Lee, Ji-Hye, Jang, Si-Hyong, Kang, Jeong Suk, Gil, Hyo-Wook, Park, Samel, Lee, Eun Young
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5874023/
https://www.ncbi.nlm.nih.gov/pubmed/29590173
http://dx.doi.org/10.1371/journal.pone.0194617
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author Cho, Nam-Jun
Han, Dong-Jae
Lee, Ji-Hye
Jang, Si-Hyong
Kang, Jeong Suk
Gil, Hyo-Wook
Park, Samel
Lee, Eun Young
author_facet Cho, Nam-Jun
Han, Dong-Jae
Lee, Ji-Hye
Jang, Si-Hyong
Kang, Jeong Suk
Gil, Hyo-Wook
Park, Samel
Lee, Eun Young
author_sort Cho, Nam-Jun
collection PubMed
description Klotho deficiency is relevant to renal fibrosis and podocyte injury in vivo and in vitro. We examined whether histological findings of renal biopsy specimens were associated with the levels of soluble klotho in humans. We investigated renal biopsy specimens of 67 patients and detailed microscopic findings were reviewed. Soluble serum/urinary klotho and urinary angiotensinogen were assessed by enzyme-linked immunosorbent assays, and tissue klotho expression was assessed by immunohistochemical staining. The median age of the study participants was 35.6 years. High serum klotho levels (≥14 pg/mL) were associated with decreased odds ratios (ORs) of interstitial fibrosis (OR = 0.019, P = 0.003) and segmental sclerosis (OR = 0.190, P = 0.022) in multivariable logistic regression analysis. Patients with a lower urinary klotho-to-creatinine ratio (UKCR) were significantly more likely to have diffuse foot process effacement (OR = 0.450, P = 0.010). The area under the receiver-operating characteristic curve (AUC) of serum klotho for predicting interstitial fibrosis was 0.920 (95% CI, 0.844–0.996), and the best cut-off value of serum klotho was 138.1 pg/mL. The AUC of UKCR for predicting diffuse foot process effacement was 0.754 (95% CI, 0.636–0.872), and the best cut-off value of UKCR was 96.7 pg/mgCr. Urinary angiotensinogen-to-creatinine ratio was not associated with serum klotho, UKCR, or any pathological finding. Our data suggested that soluble serum and urinary klotho levels represent a potential biomarker to predict renal fibrosis and podocyte injury in humans.
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spelling pubmed-58740232018-04-06 Soluble klotho as a marker of renal fibrosis and podocyte injuries in human kidneys Cho, Nam-Jun Han, Dong-Jae Lee, Ji-Hye Jang, Si-Hyong Kang, Jeong Suk Gil, Hyo-Wook Park, Samel Lee, Eun Young PLoS One Research Article Klotho deficiency is relevant to renal fibrosis and podocyte injury in vivo and in vitro. We examined whether histological findings of renal biopsy specimens were associated with the levels of soluble klotho in humans. We investigated renal biopsy specimens of 67 patients and detailed microscopic findings were reviewed. Soluble serum/urinary klotho and urinary angiotensinogen were assessed by enzyme-linked immunosorbent assays, and tissue klotho expression was assessed by immunohistochemical staining. The median age of the study participants was 35.6 years. High serum klotho levels (≥14 pg/mL) were associated with decreased odds ratios (ORs) of interstitial fibrosis (OR = 0.019, P = 0.003) and segmental sclerosis (OR = 0.190, P = 0.022) in multivariable logistic regression analysis. Patients with a lower urinary klotho-to-creatinine ratio (UKCR) were significantly more likely to have diffuse foot process effacement (OR = 0.450, P = 0.010). The area under the receiver-operating characteristic curve (AUC) of serum klotho for predicting interstitial fibrosis was 0.920 (95% CI, 0.844–0.996), and the best cut-off value of serum klotho was 138.1 pg/mL. The AUC of UKCR for predicting diffuse foot process effacement was 0.754 (95% CI, 0.636–0.872), and the best cut-off value of UKCR was 96.7 pg/mgCr. Urinary angiotensinogen-to-creatinine ratio was not associated with serum klotho, UKCR, or any pathological finding. Our data suggested that soluble serum and urinary klotho levels represent a potential biomarker to predict renal fibrosis and podocyte injury in humans. Public Library of Science 2018-03-28 /pmc/articles/PMC5874023/ /pubmed/29590173 http://dx.doi.org/10.1371/journal.pone.0194617 Text en © 2018 Cho et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Cho, Nam-Jun
Han, Dong-Jae
Lee, Ji-Hye
Jang, Si-Hyong
Kang, Jeong Suk
Gil, Hyo-Wook
Park, Samel
Lee, Eun Young
Soluble klotho as a marker of renal fibrosis and podocyte injuries in human kidneys
title Soluble klotho as a marker of renal fibrosis and podocyte injuries in human kidneys
title_full Soluble klotho as a marker of renal fibrosis and podocyte injuries in human kidneys
title_fullStr Soluble klotho as a marker of renal fibrosis and podocyte injuries in human kidneys
title_full_unstemmed Soluble klotho as a marker of renal fibrosis and podocyte injuries in human kidneys
title_short Soluble klotho as a marker of renal fibrosis and podocyte injuries in human kidneys
title_sort soluble klotho as a marker of renal fibrosis and podocyte injuries in human kidneys
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5874023/
https://www.ncbi.nlm.nih.gov/pubmed/29590173
http://dx.doi.org/10.1371/journal.pone.0194617
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