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Recombinant Zika virus envelope protein elicited protective immunity against Zika virus in immunocompetent mice

Zika virus (ZIKV) has caused great public concerns due to its recent large outbreaks and a close association with microcephaly in fetus and Guillain-Barre syndrome in adults. Rapid development of vaccines against ZIKV is a public health priority. To this end, we have constructed and purified recombi...

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Detalles Bibliográficos
Autores principales: Liang, Huabin, Yang, Ruoheng, Liu, Zhihua, Li, Min, Liu, Haitao, Jin, Xia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5874044/
https://www.ncbi.nlm.nih.gov/pubmed/29590178
http://dx.doi.org/10.1371/journal.pone.0194860
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author Liang, Huabin
Yang, Ruoheng
Liu, Zhihua
Li, Min
Liu, Haitao
Jin, Xia
author_facet Liang, Huabin
Yang, Ruoheng
Liu, Zhihua
Li, Min
Liu, Haitao
Jin, Xia
author_sort Liang, Huabin
collection PubMed
description Zika virus (ZIKV) has caused great public concerns due to its recent large outbreaks and a close association with microcephaly in fetus and Guillain-Barre syndrome in adults. Rapid development of vaccines against ZIKV is a public health priority. To this end, we have constructed and purified recombinant ZIKV envelope protein using both prokaryotic and eukaryotic expression systems, and then tested their immunogenicity and protective efficacy in immune competent mice. Both protein immunogens elicited humoral and cellular immune responses, and protected immune competent mice from ZIKV challenge in vivo. These products could be further evaluated either as stand-alone vaccine candidate, or used in a prime-and-boost regimen with other forms of ZIKV vaccine.
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spelling pubmed-58740442018-04-06 Recombinant Zika virus envelope protein elicited protective immunity against Zika virus in immunocompetent mice Liang, Huabin Yang, Ruoheng Liu, Zhihua Li, Min Liu, Haitao Jin, Xia PLoS One Research Article Zika virus (ZIKV) has caused great public concerns due to its recent large outbreaks and a close association with microcephaly in fetus and Guillain-Barre syndrome in adults. Rapid development of vaccines against ZIKV is a public health priority. To this end, we have constructed and purified recombinant ZIKV envelope protein using both prokaryotic and eukaryotic expression systems, and then tested their immunogenicity and protective efficacy in immune competent mice. Both protein immunogens elicited humoral and cellular immune responses, and protected immune competent mice from ZIKV challenge in vivo. These products could be further evaluated either as stand-alone vaccine candidate, or used in a prime-and-boost regimen with other forms of ZIKV vaccine. Public Library of Science 2018-03-28 /pmc/articles/PMC5874044/ /pubmed/29590178 http://dx.doi.org/10.1371/journal.pone.0194860 Text en © 2018 Liang et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Liang, Huabin
Yang, Ruoheng
Liu, Zhihua
Li, Min
Liu, Haitao
Jin, Xia
Recombinant Zika virus envelope protein elicited protective immunity against Zika virus in immunocompetent mice
title Recombinant Zika virus envelope protein elicited protective immunity against Zika virus in immunocompetent mice
title_full Recombinant Zika virus envelope protein elicited protective immunity against Zika virus in immunocompetent mice
title_fullStr Recombinant Zika virus envelope protein elicited protective immunity against Zika virus in immunocompetent mice
title_full_unstemmed Recombinant Zika virus envelope protein elicited protective immunity against Zika virus in immunocompetent mice
title_short Recombinant Zika virus envelope protein elicited protective immunity against Zika virus in immunocompetent mice
title_sort recombinant zika virus envelope protein elicited protective immunity against zika virus in immunocompetent mice
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5874044/
https://www.ncbi.nlm.nih.gov/pubmed/29590178
http://dx.doi.org/10.1371/journal.pone.0194860
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