Cargando…

Versatile approach for functional analysis of human proteins and efficient stable cell line generation using FLP-mediated recombination system

Deciphering a function of a given protein requires investigating various biological aspects. Usually, the protein of interest is expressed with a fusion tag that aids or allows subsequent analyses. Additionally, downregulation or inactivation of the studied gene enables functional studies. Developme...

Descripción completa

Detalles Bibliográficos
Autores principales:	Szczesny, Roman J., Kowalska, Katarzyna, Klosowska-Kosicka, Kamila, Chlebowski, Aleksander, Owczarek, Ewelina P., Warkocki, Zbigniew, Kulinski, Tomasz M., Adamska, Dorota, Affek, Kamila, Jedroszkowiak, Agata, Kotrys, Anna V., Tomecki, Rafal, Krawczyk, Pawel S., Borowski, Lukasz S., Dziembowski, Andrzej
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Public Library of Science 2018
Materias:	Research Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5874048/ https://www.ncbi.nlm.nih.gov/pubmed/29590189 http://dx.doi.org/10.1371/journal.pone.0194887

Ejemplares similares

Landscape of functional interactions of human processive ribonucleases revealed by high-throughput siRNA screenings
por: Hojka-Osinska, Anna, et al.
Publicado: (2021)

Perlman syndrome nuclease DIS3L2 controls cytoplasmic non-coding RNAs and provides surveillance pathway for maturing snRNAs
por: Łabno, Anna, et al.
Publicado: (2016)

Human REXO2 controls short mitochondrial RNAs generated by mtRNA processing and decay machinery to prevent accumulation of double-stranded RNA
por: Szewczyk, Maciej, et al.
Publicado: (2020)

A short splicing isoform of HBS1L links the cytoplasmic exosome and SKI complexes in humans
por: Kalisiak, Katarzyna, et al.
Publicado: (2017)

DIS3 shapes the RNA polymerase II transcriptome in humans by degrading a variety of unwanted transcripts
por: Szczepińska, Teresa, et al.
Publicado: (2015)

Uridylation by TUT4/7 Restricts Retrotransposition of Human LINE-1s
por: Warkocki, Zbigniew, et al.
Publicado: (2018)

A new strategy for gene targeting and functional proteomics using the DT40 cell line
por: Orlowska, Kinga P., et al.
Publicado: (2013)

Quantitative proteomics revealed C6orf203/MTRES1 as a factor preventing stress-induced transcription deficiency in human mitochondria
por: Kotrys, Anna V, et al.
Publicado: (2019)

Multiple myeloma-associated hDIS3 mutations cause perturbations in cellular RNA metabolism and suggest hDIS3 PIN domain as a potential drug target
por: Tomecki, Rafal, et al.
Publicado: (2014)

Elimination of 01/A′–A0 pre-rRNA processing by-product in human cells involves cooperative action of two nuclear exosome-associated nucleases: RRP6 and DIS3
por: Kobyłecki, Kamil, et al.
Publicado: (2018)

Dedicated surveillance mechanism controls G-quadruplex forming non-coding RNAs in human mitochondria
por: Pietras, Zbigniew, et al.
Publicado: (2018)

Controlling the mitochondrial antisense – role of the SUV3-PNPase complex and its co-factor GRSF1 in mitochondrial RNA surveillance
por: Pietras, Zbigniew, et al.
Publicado: (2018)

Terminal nucleotidyl transferases (TENTs) in mammalian RNA metabolism
por: Warkocki, Zbigniew, et al.
Publicado: (2018)

Unraveling flp-11/flp-32 dichotomy in nematodes
por: Atkinson, Louise E., et al.
Publicado: (2016)

The RNA exosome complex central channel controls both exonuclease and endonuclease Dis3 activities in vivo and in vitro
por: Drążkowska, Karolina, et al.
Publicado: (2013)

Human mitochondrial RNA decay mediated by PNPase–hSuv3 complex takes place in distinct foci
por: Borowski, Lukasz S., et al.
Publicado: (2013)

Mitochondrial Gene Expression and Beyond—Novel Aspects of Cellular Physiology
por: Kotrys, Anna V., et al.
Publicado: (2019)

Flp and Cre expressed from Flp–2A–Cre and Flp–IRES–Cre transcription units mediate the highest level of dual recombinase-mediated cassette exchange
por: Anderson, Rachelle P., et al.
Publicado: (2012)

Two novel C-terminal frameshift mutations in the β-globin gene lead to rapid mRNA decay
por: Rawa, Katarzyna, et al.
Publicado: (2017)

Identification of a novel human mitochondrial endo-/exonuclease Ddk1/c20orf72 necessary for maintenance of proper 7S DNA levels
por: Szczesny, Roman J., et al.
Publicado: (2013)

Simultaneous RNAi Knockdown of Three FMRFamide-Like Peptide Genes, Mi-flp1, Mi-flp12, and Mi-flp18 Provides Resistance to Root-Knot Nematode, Meloidogyne incognita
por: Banakar, Prakash, et al.
Publicado: (2020)

Structure and mechanism of CutA, RNA nucleotidyl transferase with an unusual preference for cytosine
por: Malik, Deepshikha, et al.
Publicado: (2020)

Global view on the metabolism of RNA poly(A) tails in yeast Saccharomyces cerevisiae
por: Tudek, Agnieszka, et al.
Publicado: (2021)

Biochemical and structural bioinformatics studies of fungal CutA nucleotidyltransferases explain their unusual specificity toward CTP and increased tendency for cytidine incorporation at the 3′-terminal positions of synthesized tails
por: Kobyłecki, Kamil, et al.
Publicado: (2017)

The FLP microsatellite platform: flight operations manual
por: Eickhoff, Jens
Publicado: (2016)

Genome targeting by hybrid Flp-TAL recombinases
por: Voziyanova, Eugenia, et al.
Publicado: (2020)

Identification of neuropeptides, flp-1 and flp-12 targeting neuromuscular system of rice root knot nematode (RRKN) Meloidogyne graminicola
por: Rao, Uma, et al.
Publicado: (2013)

Effects of interactions between variation in dopaminergic genes, traumatic life events, and anomalous self-experiences on psychosis proneness: Results from a cross-sectional study in a nonclinical sample
por: Frydecka, Dorota, et al.
Publicado: (2020)

Gene Activation Using FLP Recombinase in C. elegans
por: Davis, M. Wayne, et al.
Publicado: (2008)

The ALICE detector's First Level Processor (FLP) system
por: Chapeland, Sylvain
Publicado: (2020)

Family of FLP Peptides in Caenorhabditis elegans and Related Nematodes
por: Li, Chris, et al.
Publicado: (2014)

Novel Flp pilus biogenesis-dependent natural transformation
por: Angelov, Angel, et al.
Publicado: (2015)

FLP‐Catalyzed Transfer Hydrogenation of Silyl Enol Ethers
por: Khan, Imtiaz, et al.
Publicado: (2018)

Utility of Host Delivered RNAi of Two FMRF Amide Like Peptides, flp-14 and flp-18, for the Management of Root Knot Nematode, Meloidogyne incognita
por: Papolu, Pradeep Kumar, et al.
Publicado: (2013)

Modulation of Locomotion and Reproduction by FLP Neuropeptides in the Nematode Caenorhabditis elegans
por: Chang, Yan-Jung, et al.
Publicado: (2015)

Phosphorylcholine is located in Aggregatibacter actinomycetemcomitans fimbrial protein Flp 1
por: Ihalin, Riikka, et al.
Publicado: (2018)

FlpStop, a tool for conditional gene control in Drosophila
por: Fisher, Yvette E, et al.
Publicado: (2017)

A double-Flp-in method for stable overexpression of two genes
por: Jensen, Ole, et al.
Publicado: (2020)

Efficient FLP-mediated germ-line recombination in C. elegans
por: Macías-León, Javier, et al.
Publicado: (2018)

Host-Induced Silencing of FMRFamide-Like Peptide Genes, flp-1 and flp-12, in Rice Impairs Reproductive Fitness of the Root-Knot Nematode Meloidogyne graminicola
por: Hada, Alkesh, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_s3v4h6d4qpt4rhjdu5s1jj81pl