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Quantification of Phenolic Compounds and In Vitro Radical Scavenging Abilities with Leaf Extracts from Two Varieties of Psidium guajava L.

Guava leaf (Psidium guajava L.) extracts are used in both traditional medicine and the pharmaceutical industry. The antioxidant compounds in P. guajava leaves can have positive effects including anti-inflammatory, anti-hyperglycemic, hepatoprotective, analgesic, anti-cancer effects, as well as prote...

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Autores principales: Camarena-Tello, Julio César, Martínez-Flores, Héctor Eduardo, Garnica-Romo, Ma. Guadalupe, Padilla-Ramírez, José Saúl, Saavedra-Molina, Alfredo, Alvarez-Cortes, Osvaldo, Bartolomé-Camacho, María Carmen, Rodiles-López, José Octavio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5874520/
https://www.ncbi.nlm.nih.gov/pubmed/29495514
http://dx.doi.org/10.3390/antiox7030034
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author Camarena-Tello, Julio César
Martínez-Flores, Héctor Eduardo
Garnica-Romo, Ma. Guadalupe
Padilla-Ramírez, José Saúl
Saavedra-Molina, Alfredo
Alvarez-Cortes, Osvaldo
Bartolomé-Camacho, María Carmen
Rodiles-López, José Octavio
author_facet Camarena-Tello, Julio César
Martínez-Flores, Héctor Eduardo
Garnica-Romo, Ma. Guadalupe
Padilla-Ramírez, José Saúl
Saavedra-Molina, Alfredo
Alvarez-Cortes, Osvaldo
Bartolomé-Camacho, María Carmen
Rodiles-López, José Octavio
author_sort Camarena-Tello, Julio César
collection PubMed
description Guava leaf (Psidium guajava L.) extracts are used in both traditional medicine and the pharmaceutical industry. The antioxidant compounds in P. guajava leaves can have positive effects including anti-inflammatory, anti-hyperglycemic, hepatoprotective, analgesic, anti-cancer effects, as well as protecting against cardiovascular diseases. In the present study, phenolic compounds and in vitro antioxidant capacity were measured in extracts obtained with polar and non-polar solvents from leaves of two varieties of guava, Calvillo Siglo XXI and Hidrozac. The quantity of total phenolics and total flavonoids were expressed as equivalents of gallic acid and quercetin, respectively. Hydroxyl radical, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and Oxygen Radical Absorbance Capacity using fluorescein (ORAC-FL) in vitro tests were used to assess the radical scavenging abilities of the extracts. The total phenolics were higher in the aqueous fraction of the variety Calvillo Siglo XXI, while in the Hidrozac variety total phenolics were higher in the acetone and chloroform fractions. Total flavonoids were higher in all fractions in the variety Calvillo Siglo XXI. Total phenolics showed a highly positive correlation for ORAC-FL, and a moderately positive correlation with hydroxyl radicals. Finally, total flavonoids showed a slightly positive correlation for ORAC-FL and hydroxyl radicals. Both varieties of guava leaf extract showed excellent antioxidant properties.
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spelling pubmed-58745202018-04-02 Quantification of Phenolic Compounds and In Vitro Radical Scavenging Abilities with Leaf Extracts from Two Varieties of Psidium guajava L. Camarena-Tello, Julio César Martínez-Flores, Héctor Eduardo Garnica-Romo, Ma. Guadalupe Padilla-Ramírez, José Saúl Saavedra-Molina, Alfredo Alvarez-Cortes, Osvaldo Bartolomé-Camacho, María Carmen Rodiles-López, José Octavio Antioxidants (Basel) Article Guava leaf (Psidium guajava L.) extracts are used in both traditional medicine and the pharmaceutical industry. The antioxidant compounds in P. guajava leaves can have positive effects including anti-inflammatory, anti-hyperglycemic, hepatoprotective, analgesic, anti-cancer effects, as well as protecting against cardiovascular diseases. In the present study, phenolic compounds and in vitro antioxidant capacity were measured in extracts obtained with polar and non-polar solvents from leaves of two varieties of guava, Calvillo Siglo XXI and Hidrozac. The quantity of total phenolics and total flavonoids were expressed as equivalents of gallic acid and quercetin, respectively. Hydroxyl radical, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), 2,2-diphenyl-1-picrylhydrazyl (DPPH), and Oxygen Radical Absorbance Capacity using fluorescein (ORAC-FL) in vitro tests were used to assess the radical scavenging abilities of the extracts. The total phenolics were higher in the aqueous fraction of the variety Calvillo Siglo XXI, while in the Hidrozac variety total phenolics were higher in the acetone and chloroform fractions. Total flavonoids were higher in all fractions in the variety Calvillo Siglo XXI. Total phenolics showed a highly positive correlation for ORAC-FL, and a moderately positive correlation with hydroxyl radicals. Finally, total flavonoids showed a slightly positive correlation for ORAC-FL and hydroxyl radicals. Both varieties of guava leaf extract showed excellent antioxidant properties. MDPI 2018-02-27 /pmc/articles/PMC5874520/ /pubmed/29495514 http://dx.doi.org/10.3390/antiox7030034 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Camarena-Tello, Julio César
Martínez-Flores, Héctor Eduardo
Garnica-Romo, Ma. Guadalupe
Padilla-Ramírez, José Saúl
Saavedra-Molina, Alfredo
Alvarez-Cortes, Osvaldo
Bartolomé-Camacho, María Carmen
Rodiles-López, José Octavio
Quantification of Phenolic Compounds and In Vitro Radical Scavenging Abilities with Leaf Extracts from Two Varieties of Psidium guajava L.
title Quantification of Phenolic Compounds and In Vitro Radical Scavenging Abilities with Leaf Extracts from Two Varieties of Psidium guajava L.
title_full Quantification of Phenolic Compounds and In Vitro Radical Scavenging Abilities with Leaf Extracts from Two Varieties of Psidium guajava L.
title_fullStr Quantification of Phenolic Compounds and In Vitro Radical Scavenging Abilities with Leaf Extracts from Two Varieties of Psidium guajava L.
title_full_unstemmed Quantification of Phenolic Compounds and In Vitro Radical Scavenging Abilities with Leaf Extracts from Two Varieties of Psidium guajava L.
title_short Quantification of Phenolic Compounds and In Vitro Radical Scavenging Abilities with Leaf Extracts from Two Varieties of Psidium guajava L.
title_sort quantification of phenolic compounds and in vitro radical scavenging abilities with leaf extracts from two varieties of psidium guajava l.
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5874520/
https://www.ncbi.nlm.nih.gov/pubmed/29495514
http://dx.doi.org/10.3390/antiox7030034
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