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The Determination of Blood Glucose Lowering and Metabolic Effects of Mespilus germanica L. Hydroacetonic Extract on Streptozocin-Induced Diabetic Balb/c Mice

Background: The serum glucose lowering, normalization animal body weight, and antioxidative stress effects of Mespilus germanica L. leaf extract were investigated in normal and streptozotocin-induced Balb/C mice. Methods: The phenol and flavonoid of the leaves of M. germanica were extracted by perco...

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Autores principales: Shafiee, Fatemeh, Khoshvishkaie, Elnaz, Davoodi, Ali, Dashti Kalantar, Ayat, Bakhshi Jouybari, Hossein, Ataee, Ramin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5874566/
https://www.ncbi.nlm.nih.gov/pubmed/29301240
http://dx.doi.org/10.3390/medicines5010001
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author Shafiee, Fatemeh
Khoshvishkaie, Elnaz
Davoodi, Ali
Dashti Kalantar, Ayat
Bakhshi Jouybari, Hossein
Ataee, Ramin
author_facet Shafiee, Fatemeh
Khoshvishkaie, Elnaz
Davoodi, Ali
Dashti Kalantar, Ayat
Bakhshi Jouybari, Hossein
Ataee, Ramin
author_sort Shafiee, Fatemeh
collection PubMed
description Background: The serum glucose lowering, normalization animal body weight, and antioxidative stress effects of Mespilus germanica L. leaf extract were investigated in normal and streptozotocin-induced Balb/C mice. Methods: The phenol and flavonoid of the leaves of M. germanica were extracted by percolation and concentrated using a rotary evaporator. Its total phenol and flavonoid content was determined using folin and aluminum chloride methods, respectively. The study was conducted on 48 matured male Balb/C mice (20–30 g) divided into 6 groups (n = 8). Diabetes mellitus was induced by single intraperitoneal injection of 35 mg/kg of streptozotocin (STZ). Extracts of Mespilus germanica were used orally at the dose of 50, 100, and 200 mg/kg body weight per day for 21 days. Results: Oral administrations of the M. germanica L. leaf extract significantly decreased serum glucose, oxidative stress, and lipid peroxidation and maintained animal body weight during treatment period (p < 0.05) compared to metformin (200 mg/kg) in over 100 mg/kg, 200 mg/kg, and 50 mg/kg dosages, respectively. Conclusions: The present study indicated that the Mespilus germanica leaf extract significantly decreased serum glucose and maintained normal body weight in Balb/C diabetic mice.
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spelling pubmed-58745662018-03-30 The Determination of Blood Glucose Lowering and Metabolic Effects of Mespilus germanica L. Hydroacetonic Extract on Streptozocin-Induced Diabetic Balb/c Mice Shafiee, Fatemeh Khoshvishkaie, Elnaz Davoodi, Ali Dashti Kalantar, Ayat Bakhshi Jouybari, Hossein Ataee, Ramin Medicines (Basel) Article Background: The serum glucose lowering, normalization animal body weight, and antioxidative stress effects of Mespilus germanica L. leaf extract were investigated in normal and streptozotocin-induced Balb/C mice. Methods: The phenol and flavonoid of the leaves of M. germanica were extracted by percolation and concentrated using a rotary evaporator. Its total phenol and flavonoid content was determined using folin and aluminum chloride methods, respectively. The study was conducted on 48 matured male Balb/C mice (20–30 g) divided into 6 groups (n = 8). Diabetes mellitus was induced by single intraperitoneal injection of 35 mg/kg of streptozotocin (STZ). Extracts of Mespilus germanica were used orally at the dose of 50, 100, and 200 mg/kg body weight per day for 21 days. Results: Oral administrations of the M. germanica L. leaf extract significantly decreased serum glucose, oxidative stress, and lipid peroxidation and maintained animal body weight during treatment period (p < 0.05) compared to metformin (200 mg/kg) in over 100 mg/kg, 200 mg/kg, and 50 mg/kg dosages, respectively. Conclusions: The present study indicated that the Mespilus germanica leaf extract significantly decreased serum glucose and maintained normal body weight in Balb/C diabetic mice. MDPI 2018-01-01 /pmc/articles/PMC5874566/ /pubmed/29301240 http://dx.doi.org/10.3390/medicines5010001 Text en © 2017 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shafiee, Fatemeh
Khoshvishkaie, Elnaz
Davoodi, Ali
Dashti Kalantar, Ayat
Bakhshi Jouybari, Hossein
Ataee, Ramin
The Determination of Blood Glucose Lowering and Metabolic Effects of Mespilus germanica L. Hydroacetonic Extract on Streptozocin-Induced Diabetic Balb/c Mice
title The Determination of Blood Glucose Lowering and Metabolic Effects of Mespilus germanica L. Hydroacetonic Extract on Streptozocin-Induced Diabetic Balb/c Mice
title_full The Determination of Blood Glucose Lowering and Metabolic Effects of Mespilus germanica L. Hydroacetonic Extract on Streptozocin-Induced Diabetic Balb/c Mice
title_fullStr The Determination of Blood Glucose Lowering and Metabolic Effects of Mespilus germanica L. Hydroacetonic Extract on Streptozocin-Induced Diabetic Balb/c Mice
title_full_unstemmed The Determination of Blood Glucose Lowering and Metabolic Effects of Mespilus germanica L. Hydroacetonic Extract on Streptozocin-Induced Diabetic Balb/c Mice
title_short The Determination of Blood Glucose Lowering and Metabolic Effects of Mespilus germanica L. Hydroacetonic Extract on Streptozocin-Induced Diabetic Balb/c Mice
title_sort determination of blood glucose lowering and metabolic effects of mespilus germanica l. hydroacetonic extract on streptozocin-induced diabetic balb/c mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5874566/
https://www.ncbi.nlm.nih.gov/pubmed/29301240
http://dx.doi.org/10.3390/medicines5010001
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