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Raman Imaging of Plant Cell Walls in Sections of Cucumis sativus
Raman microspectra combine information on chemical composition of plant tissues with spatial information. The contributions from the building blocks of the cell walls in the Raman spectra of plant tissues can vary in the microscopic sub-structures of the tissue. Here, we discuss the analysis of 55 R...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5874596/ https://www.ncbi.nlm.nih.gov/pubmed/29370089 http://dx.doi.org/10.3390/plants7010007 |
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author | Zeise, Ingrid Heiner, Zsuzsanna Holz, Sabine Joester, Maike Büttner, Carmen Kneipp, Janina |
author_facet | Zeise, Ingrid Heiner, Zsuzsanna Holz, Sabine Joester, Maike Büttner, Carmen Kneipp, Janina |
author_sort | Zeise, Ingrid |
collection | PubMed |
description | Raman microspectra combine information on chemical composition of plant tissues with spatial information. The contributions from the building blocks of the cell walls in the Raman spectra of plant tissues can vary in the microscopic sub-structures of the tissue. Here, we discuss the analysis of 55 Raman maps of root, stem, and leaf tissues of Cucumis sativus, using different spectral contributions from cellulose and lignin in both univariate and multivariate imaging methods. Imaging based on hierarchical cluster analysis (HCA) and principal component analysis (PCA) indicates different substructures in the xylem cell walls of the different tissues. Using specific signals from the cell wall spectra, analysis of the whole set of different tissue sections based on the Raman images reveals differences in xylem tissue morphology. Due to the specifics of excitation of the Raman spectra in the visible wavelength range (532 nm), which is, e.g., in resonance with carotenoid species, effects of photobleaching and the possibility of exploiting depletion difference spectra for molecular characterization in Raman imaging of plants are discussed. The reported results provide both, specific information on the molecular composition of cucumber tissue Raman spectra, and general directions for future imaging studies in plant tissues. |
format | Online Article Text |
id | pubmed-5874596 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-58745962018-04-02 Raman Imaging of Plant Cell Walls in Sections of Cucumis sativus Zeise, Ingrid Heiner, Zsuzsanna Holz, Sabine Joester, Maike Büttner, Carmen Kneipp, Janina Plants (Basel) Article Raman microspectra combine information on chemical composition of plant tissues with spatial information. The contributions from the building blocks of the cell walls in the Raman spectra of plant tissues can vary in the microscopic sub-structures of the tissue. Here, we discuss the analysis of 55 Raman maps of root, stem, and leaf tissues of Cucumis sativus, using different spectral contributions from cellulose and lignin in both univariate and multivariate imaging methods. Imaging based on hierarchical cluster analysis (HCA) and principal component analysis (PCA) indicates different substructures in the xylem cell walls of the different tissues. Using specific signals from the cell wall spectra, analysis of the whole set of different tissue sections based on the Raman images reveals differences in xylem tissue morphology. Due to the specifics of excitation of the Raman spectra in the visible wavelength range (532 nm), which is, e.g., in resonance with carotenoid species, effects of photobleaching and the possibility of exploiting depletion difference spectra for molecular characterization in Raman imaging of plants are discussed. The reported results provide both, specific information on the molecular composition of cucumber tissue Raman spectra, and general directions for future imaging studies in plant tissues. MDPI 2018-01-25 /pmc/articles/PMC5874596/ /pubmed/29370089 http://dx.doi.org/10.3390/plants7010007 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Zeise, Ingrid Heiner, Zsuzsanna Holz, Sabine Joester, Maike Büttner, Carmen Kneipp, Janina Raman Imaging of Plant Cell Walls in Sections of Cucumis sativus |
title | Raman Imaging of Plant Cell Walls in Sections of Cucumis sativus |
title_full | Raman Imaging of Plant Cell Walls in Sections of Cucumis sativus |
title_fullStr | Raman Imaging of Plant Cell Walls in Sections of Cucumis sativus |
title_full_unstemmed | Raman Imaging of Plant Cell Walls in Sections of Cucumis sativus |
title_short | Raman Imaging of Plant Cell Walls in Sections of Cucumis sativus |
title_sort | raman imaging of plant cell walls in sections of cucumis sativus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5874596/ https://www.ncbi.nlm.nih.gov/pubmed/29370089 http://dx.doi.org/10.3390/plants7010007 |
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