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Raman Imaging of Plant Cell Walls in Sections of Cucumis sativus

Raman microspectra combine information on chemical composition of plant tissues with spatial information. The contributions from the building blocks of the cell walls in the Raman spectra of plant tissues can vary in the microscopic sub-structures of the tissue. Here, we discuss the analysis of 55 R...

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Autores principales: Zeise, Ingrid, Heiner, Zsuzsanna, Holz, Sabine, Joester, Maike, Büttner, Carmen, Kneipp, Janina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5874596/
https://www.ncbi.nlm.nih.gov/pubmed/29370089
http://dx.doi.org/10.3390/plants7010007
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author Zeise, Ingrid
Heiner, Zsuzsanna
Holz, Sabine
Joester, Maike
Büttner, Carmen
Kneipp, Janina
author_facet Zeise, Ingrid
Heiner, Zsuzsanna
Holz, Sabine
Joester, Maike
Büttner, Carmen
Kneipp, Janina
author_sort Zeise, Ingrid
collection PubMed
description Raman microspectra combine information on chemical composition of plant tissues with spatial information. The contributions from the building blocks of the cell walls in the Raman spectra of plant tissues can vary in the microscopic sub-structures of the tissue. Here, we discuss the analysis of 55 Raman maps of root, stem, and leaf tissues of Cucumis sativus, using different spectral contributions from cellulose and lignin in both univariate and multivariate imaging methods. Imaging based on hierarchical cluster analysis (HCA) and principal component analysis (PCA) indicates different substructures in the xylem cell walls of the different tissues. Using specific signals from the cell wall spectra, analysis of the whole set of different tissue sections based on the Raman images reveals differences in xylem tissue morphology. Due to the specifics of excitation of the Raman spectra in the visible wavelength range (532 nm), which is, e.g., in resonance with carotenoid species, effects of photobleaching and the possibility of exploiting depletion difference spectra for molecular characterization in Raman imaging of plants are discussed. The reported results provide both, specific information on the molecular composition of cucumber tissue Raman spectra, and general directions for future imaging studies in plant tissues.
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spelling pubmed-58745962018-04-02 Raman Imaging of Plant Cell Walls in Sections of Cucumis sativus Zeise, Ingrid Heiner, Zsuzsanna Holz, Sabine Joester, Maike Büttner, Carmen Kneipp, Janina Plants (Basel) Article Raman microspectra combine information on chemical composition of plant tissues with spatial information. The contributions from the building blocks of the cell walls in the Raman spectra of plant tissues can vary in the microscopic sub-structures of the tissue. Here, we discuss the analysis of 55 Raman maps of root, stem, and leaf tissues of Cucumis sativus, using different spectral contributions from cellulose and lignin in both univariate and multivariate imaging methods. Imaging based on hierarchical cluster analysis (HCA) and principal component analysis (PCA) indicates different substructures in the xylem cell walls of the different tissues. Using specific signals from the cell wall spectra, analysis of the whole set of different tissue sections based on the Raman images reveals differences in xylem tissue morphology. Due to the specifics of excitation of the Raman spectra in the visible wavelength range (532 nm), which is, e.g., in resonance with carotenoid species, effects of photobleaching and the possibility of exploiting depletion difference spectra for molecular characterization in Raman imaging of plants are discussed. The reported results provide both, specific information on the molecular composition of cucumber tissue Raman spectra, and general directions for future imaging studies in plant tissues. MDPI 2018-01-25 /pmc/articles/PMC5874596/ /pubmed/29370089 http://dx.doi.org/10.3390/plants7010007 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zeise, Ingrid
Heiner, Zsuzsanna
Holz, Sabine
Joester, Maike
Büttner, Carmen
Kneipp, Janina
Raman Imaging of Plant Cell Walls in Sections of Cucumis sativus
title Raman Imaging of Plant Cell Walls in Sections of Cucumis sativus
title_full Raman Imaging of Plant Cell Walls in Sections of Cucumis sativus
title_fullStr Raman Imaging of Plant Cell Walls in Sections of Cucumis sativus
title_full_unstemmed Raman Imaging of Plant Cell Walls in Sections of Cucumis sativus
title_short Raman Imaging of Plant Cell Walls in Sections of Cucumis sativus
title_sort raman imaging of plant cell walls in sections of cucumis sativus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5874596/
https://www.ncbi.nlm.nih.gov/pubmed/29370089
http://dx.doi.org/10.3390/plants7010007
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