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Comparison of Rumen and Manure Microbiomes and Implications for the Inoculation of Anaerobic Digesters

Cattle manure is frequently used as an inoculum for the start-up of agricultural biogas plants or as a co-substrate in the anaerobic digestion of lignocellulosic feedstock. Ruminal microbiota are considered to be effective plant fiber degraders, but the microbes contained in manure do not necessaril...

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Autores principales: Ozbayram, Emine Gozde, Ince, Orhan, Ince, Bahar, Harms, Hauke, Kleinsteuber, Sabine
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5874629/
https://www.ncbi.nlm.nih.gov/pubmed/29443879
http://dx.doi.org/10.3390/microorganisms6010015
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author Ozbayram, Emine Gozde
Ince, Orhan
Ince, Bahar
Harms, Hauke
Kleinsteuber, Sabine
author_facet Ozbayram, Emine Gozde
Ince, Orhan
Ince, Bahar
Harms, Hauke
Kleinsteuber, Sabine
author_sort Ozbayram, Emine Gozde
collection PubMed
description Cattle manure is frequently used as an inoculum for the start-up of agricultural biogas plants or as a co-substrate in the anaerobic digestion of lignocellulosic feedstock. Ruminal microbiota are considered to be effective plant fiber degraders, but the microbes contained in manure do not necessarily reflect the rumen microbiome. The aim of this study was to compare the microbial community composition of cow rumen and manure with respect to plant fiber-digesting microbes. Bacterial and methanogenic communities of rumen and manure samples were examined by 454 amplicon sequencing of bacterial 16S rRNA genes and mcrA genes, respectively. Rumen fluid samples were dominated by Prevotellaceae (29%), whereas Ruminococcaceae was the most abundant family in the manure samples (31%). Fibrobacteraceae (12%) and Bacteroidaceae (13%) were the second most abundant families in rumen fluid and manure, respectively. The high abundances of fiber-degrading bacteria belonging to Prevotellaceae and Fibrobacteraceae might explain the better performance of anaerobic digesters inoculated with rumen fluid. Members of the genus Methanobrevibacter were the predominant methanogens in the rumen fluid, whereas methanogenic communities of the manure samples were dominated by the candidate genus Methanoplasma. Our results suggest that inoculation or bioaugmentation with fiber-digesting rumen microbiota can enhance the anaerobic digestion of lignocellulosic biomass.
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spelling pubmed-58746292018-04-02 Comparison of Rumen and Manure Microbiomes and Implications for the Inoculation of Anaerobic Digesters Ozbayram, Emine Gozde Ince, Orhan Ince, Bahar Harms, Hauke Kleinsteuber, Sabine Microorganisms Communication Cattle manure is frequently used as an inoculum for the start-up of agricultural biogas plants or as a co-substrate in the anaerobic digestion of lignocellulosic feedstock. Ruminal microbiota are considered to be effective plant fiber degraders, but the microbes contained in manure do not necessarily reflect the rumen microbiome. The aim of this study was to compare the microbial community composition of cow rumen and manure with respect to plant fiber-digesting microbes. Bacterial and methanogenic communities of rumen and manure samples were examined by 454 amplicon sequencing of bacterial 16S rRNA genes and mcrA genes, respectively. Rumen fluid samples were dominated by Prevotellaceae (29%), whereas Ruminococcaceae was the most abundant family in the manure samples (31%). Fibrobacteraceae (12%) and Bacteroidaceae (13%) were the second most abundant families in rumen fluid and manure, respectively. The high abundances of fiber-degrading bacteria belonging to Prevotellaceae and Fibrobacteraceae might explain the better performance of anaerobic digesters inoculated with rumen fluid. Members of the genus Methanobrevibacter were the predominant methanogens in the rumen fluid, whereas methanogenic communities of the manure samples were dominated by the candidate genus Methanoplasma. Our results suggest that inoculation or bioaugmentation with fiber-digesting rumen microbiota can enhance the anaerobic digestion of lignocellulosic biomass. MDPI 2018-02-14 /pmc/articles/PMC5874629/ /pubmed/29443879 http://dx.doi.org/10.3390/microorganisms6010015 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Ozbayram, Emine Gozde
Ince, Orhan
Ince, Bahar
Harms, Hauke
Kleinsteuber, Sabine
Comparison of Rumen and Manure Microbiomes and Implications for the Inoculation of Anaerobic Digesters
title Comparison of Rumen and Manure Microbiomes and Implications for the Inoculation of Anaerobic Digesters
title_full Comparison of Rumen and Manure Microbiomes and Implications for the Inoculation of Anaerobic Digesters
title_fullStr Comparison of Rumen and Manure Microbiomes and Implications for the Inoculation of Anaerobic Digesters
title_full_unstemmed Comparison of Rumen and Manure Microbiomes and Implications for the Inoculation of Anaerobic Digesters
title_short Comparison of Rumen and Manure Microbiomes and Implications for the Inoculation of Anaerobic Digesters
title_sort comparison of rumen and manure microbiomes and implications for the inoculation of anaerobic digesters
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5874629/
https://www.ncbi.nlm.nih.gov/pubmed/29443879
http://dx.doi.org/10.3390/microorganisms6010015
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