Identification of sucA, Encoding β-Fructofuranosidase, in Rhizopus microsporus

Rhizopus microsporus NBRC 32995 was found to hydrolyze fructooligosaccharides (FOS), as well as sucrose, almost completely into monosaccharides through the production of sufficient amounts of organic acids, indicating that the complete hydrolysis of FOS was caused by the secretion of β-fructofuranos...

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Detalles Bibliográficos
Autores principales: Orikasa, Yoshitake, Oda, Yuji, Ohwada, Takuji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5874640/
https://www.ncbi.nlm.nih.gov/pubmed/29534041
http://dx.doi.org/10.3390/microorganisms6010026
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author Orikasa, Yoshitake
Oda, Yuji
Ohwada, Takuji
author_facet Orikasa, Yoshitake
Oda, Yuji
Ohwada, Takuji
author_sort Orikasa, Yoshitake
collection PubMed
description Rhizopus microsporus NBRC 32995 was found to hydrolyze fructooligosaccharides (FOS), as well as sucrose, almost completely into monosaccharides through the production of sufficient amounts of organic acids, indicating that the complete hydrolysis of FOS was caused by the secretion of β-fructofuranosidase from fungal cells. Thus, the sucA gene, encoding a β-fructofuranosidase, was amplified by degenerate PCR, and its complete nucleotide sequence was determined. The total length of the sucA gene was 1590 bp, and the SucA protein of R. microsporus NBRC 32995 belonged to clade VIa, which also contains Rhizopus delemar and is closely related to Saccharomycotina, a subdivision of the Ascomycota.
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spelling pubmed-58746402018-04-02 Identification of sucA, Encoding β-Fructofuranosidase, in Rhizopus microsporus Orikasa, Yoshitake Oda, Yuji Ohwada, Takuji Microorganisms Article Rhizopus microsporus NBRC 32995 was found to hydrolyze fructooligosaccharides (FOS), as well as sucrose, almost completely into monosaccharides through the production of sufficient amounts of organic acids, indicating that the complete hydrolysis of FOS was caused by the secretion of β-fructofuranosidase from fungal cells. Thus, the sucA gene, encoding a β-fructofuranosidase, was amplified by degenerate PCR, and its complete nucleotide sequence was determined. The total length of the sucA gene was 1590 bp, and the SucA protein of R. microsporus NBRC 32995 belonged to clade VIa, which also contains Rhizopus delemar and is closely related to Saccharomycotina, a subdivision of the Ascomycota. MDPI 2018-03-13 /pmc/articles/PMC5874640/ /pubmed/29534041 http://dx.doi.org/10.3390/microorganisms6010026 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Orikasa, Yoshitake
Oda, Yuji
Ohwada, Takuji
Identification of sucA, Encoding β-Fructofuranosidase, in Rhizopus microsporus
title Identification of sucA, Encoding β-Fructofuranosidase, in Rhizopus microsporus
title_full Identification of sucA, Encoding β-Fructofuranosidase, in Rhizopus microsporus
title_fullStr Identification of sucA, Encoding β-Fructofuranosidase, in Rhizopus microsporus
title_full_unstemmed Identification of sucA, Encoding β-Fructofuranosidase, in Rhizopus microsporus
title_short Identification of sucA, Encoding β-Fructofuranosidase, in Rhizopus microsporus
title_sort identification of suca, encoding β-fructofuranosidase, in rhizopus microsporus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5874640/
https://www.ncbi.nlm.nih.gov/pubmed/29534041
http://dx.doi.org/10.3390/microorganisms6010026
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AT ohwadatakuji identificationofsucaencodingbfructofuranosidaseinrhizopusmicrosporus

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