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Limited Interactions between Streptococcus Suis and Haemophilus Parasuis in In Vitro Co-Infection Studies

Streptococcus suis and Haemophilus parasuis are normal inhabitants of the porcine upper respiratory tract but are also among the most frequent causes of disease in weaned piglets worldwide, causing inflammatory diseases such as septicemia, meningitis and pneumonia. Using an in vitro model of infecti...

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Autores principales: Mathieu-Denoncourt, Annabelle, Letendre, Corinne, Auger, Jean-Philippe, Segura, Mariela, Aragon, Virginia, Lacouture, Sonia, Gottschalk, Marcelo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5874733/
https://www.ncbi.nlm.nih.gov/pubmed/29316613
http://dx.doi.org/10.3390/pathogens7010007
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author Mathieu-Denoncourt, Annabelle
Letendre, Corinne
Auger, Jean-Philippe
Segura, Mariela
Aragon, Virginia
Lacouture, Sonia
Gottschalk, Marcelo
author_facet Mathieu-Denoncourt, Annabelle
Letendre, Corinne
Auger, Jean-Philippe
Segura, Mariela
Aragon, Virginia
Lacouture, Sonia
Gottschalk, Marcelo
author_sort Mathieu-Denoncourt, Annabelle
collection PubMed
description Streptococcus suis and Haemophilus parasuis are normal inhabitants of the porcine upper respiratory tract but are also among the most frequent causes of disease in weaned piglets worldwide, causing inflammatory diseases such as septicemia, meningitis and pneumonia. Using an in vitro model of infection with tracheal epithelial cells or primary alveolar macrophages (PAMs), it was possible to determine the interaction between S. suis serotype 2 and H. parasuis strains with different level of virulence. Within H. parasuis strains, the low-virulence F9 strain showed higher adhesion levels to respiratory epithelial cells and greater association levels to PAMs than the high-virulence Nagasaki strain. Accordingly, the low-virulence F9 strain induced, in general, higher levels of pro-inflammatory cytokines than the virulent Nagasaki strain from both cell types. In general, S. suis adhesion levels to respiratory epithelial cells were similar to H. parasuis Nagasaki strain. Yet, S. suis strains induced a significantly lower level of pro-inflammatory cytokine expression from epithelial cells and PAMs than those observed with both H. parasuis strains. Finally, this study has shown that, overall and under the conditions used in the present study, S. suis and H. parasuis have limited in vitro interactions between them and use probably different host receptors, regardless to their level of virulence.
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spelling pubmed-58747332018-04-02 Limited Interactions between Streptococcus Suis and Haemophilus Parasuis in In Vitro Co-Infection Studies Mathieu-Denoncourt, Annabelle Letendre, Corinne Auger, Jean-Philippe Segura, Mariela Aragon, Virginia Lacouture, Sonia Gottschalk, Marcelo Pathogens Article Streptococcus suis and Haemophilus parasuis are normal inhabitants of the porcine upper respiratory tract but are also among the most frequent causes of disease in weaned piglets worldwide, causing inflammatory diseases such as septicemia, meningitis and pneumonia. Using an in vitro model of infection with tracheal epithelial cells or primary alveolar macrophages (PAMs), it was possible to determine the interaction between S. suis serotype 2 and H. parasuis strains with different level of virulence. Within H. parasuis strains, the low-virulence F9 strain showed higher adhesion levels to respiratory epithelial cells and greater association levels to PAMs than the high-virulence Nagasaki strain. Accordingly, the low-virulence F9 strain induced, in general, higher levels of pro-inflammatory cytokines than the virulent Nagasaki strain from both cell types. In general, S. suis adhesion levels to respiratory epithelial cells were similar to H. parasuis Nagasaki strain. Yet, S. suis strains induced a significantly lower level of pro-inflammatory cytokine expression from epithelial cells and PAMs than those observed with both H. parasuis strains. Finally, this study has shown that, overall and under the conditions used in the present study, S. suis and H. parasuis have limited in vitro interactions between them and use probably different host receptors, regardless to their level of virulence. MDPI 2018-01-06 /pmc/articles/PMC5874733/ /pubmed/29316613 http://dx.doi.org/10.3390/pathogens7010007 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Mathieu-Denoncourt, Annabelle
Letendre, Corinne
Auger, Jean-Philippe
Segura, Mariela
Aragon, Virginia
Lacouture, Sonia
Gottschalk, Marcelo
Limited Interactions between Streptococcus Suis and Haemophilus Parasuis in In Vitro Co-Infection Studies
title Limited Interactions between Streptococcus Suis and Haemophilus Parasuis in In Vitro Co-Infection Studies
title_full Limited Interactions between Streptococcus Suis and Haemophilus Parasuis in In Vitro Co-Infection Studies
title_fullStr Limited Interactions between Streptococcus Suis and Haemophilus Parasuis in In Vitro Co-Infection Studies
title_full_unstemmed Limited Interactions between Streptococcus Suis and Haemophilus Parasuis in In Vitro Co-Infection Studies
title_short Limited Interactions between Streptococcus Suis and Haemophilus Parasuis in In Vitro Co-Infection Studies
title_sort limited interactions between streptococcus suis and haemophilus parasuis in in vitro co-infection studies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5874733/
https://www.ncbi.nlm.nih.gov/pubmed/29316613
http://dx.doi.org/10.3390/pathogens7010007
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