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Impact of Glycerol as Carbon Source onto Specific Sugar and Inducer Uptake Rates and Inclusion Body Productivity in E. coli BL21(DE3)
The Gram-negative bacterium E. coli is the host of choice for a multitude of used recombinant proteins. Generally, cultivation is easy, media are cheap, and a high product titer can be obtained. However, harsh induction procedures using isopropyl β-d-1 thiogalactopyranoside as inducer are often refe...
Autores principales: | Kopp, Julian, Slouka, Christoph, Ulonska, Sophia, Kager, Julian, Fricke, Jens, Spadiut, Oliver, Herwig, Christoph |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2017
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5874867/ https://www.ncbi.nlm.nih.gov/pubmed/29267215 http://dx.doi.org/10.3390/bioengineering5010001 |
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