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Response of Fibroblasts MRC-5 to Flufenamic Acid-Grafted MCM-41 Nanoparticles
Recently, flufenamic acid (FFA) was discovered among fenamates as a free radical scavenger and gap junction blocker; however, its effects have only been studied in cancer cells. Normal cells in the surroundings of a tumor also respond to radiation, although they are not hit by it directly. This phen...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5874870/ https://www.ncbi.nlm.nih.gov/pubmed/29315235 http://dx.doi.org/10.3390/bioengineering5010004 |
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author | Lara, Giovanna Gomes Cipreste, Marcelo Fernandes Andrade, Gracielle Ferreira da Silva, Wellington Marcos de Sousa, Edésia Martins Barros |
author_facet | Lara, Giovanna Gomes Cipreste, Marcelo Fernandes Andrade, Gracielle Ferreira da Silva, Wellington Marcos de Sousa, Edésia Martins Barros |
author_sort | Lara, Giovanna Gomes |
collection | PubMed |
description | Recently, flufenamic acid (FFA) was discovered among fenamates as a free radical scavenger and gap junction blocker; however, its effects have only been studied in cancer cells. Normal cells in the surroundings of a tumor also respond to radiation, although they are not hit by it directly. This phenomenon is known as the bystander effect, where response molecules pass from tumor cells to normal ones, through communication channels called gap junctions. The use of the enhanced permeability and retention effect, through which drug-loaded nanoparticles smaller than 200 nm may accumulate around a tumor, can prevent the local side effect upon controlled release of the drug. The present work, aimed at functionalizing MCM-41 (Mobil Composition of Matter No. 41) silica nanoparticles with FFA and determining its biocompatibility with human fibroblasts MRC-5 (Medical Research Council cell strain 5). MCM-41, was synthesized and characterized structurally and chemically, with multiple techniques. The biocompatibility assay was performed by Live/Dead technique, with calcein and propidium–iodide. MRC-5 cells were treated with FFA-grafted MCM-41 for 48 h, and 98% of cells remained viable, without signs of necrosis or morphological changes. The results show the feasibility of MCM-41 functionalization with FFA, and its potential protection of normal cells, in comparison to the role of FFA in cancerous ones. |
format | Online Article Text |
id | pubmed-5874870 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-58748702018-04-02 Response of Fibroblasts MRC-5 to Flufenamic Acid-Grafted MCM-41 Nanoparticles Lara, Giovanna Gomes Cipreste, Marcelo Fernandes Andrade, Gracielle Ferreira da Silva, Wellington Marcos de Sousa, Edésia Martins Barros Bioengineering (Basel) Article Recently, flufenamic acid (FFA) was discovered among fenamates as a free radical scavenger and gap junction blocker; however, its effects have only been studied in cancer cells. Normal cells in the surroundings of a tumor also respond to radiation, although they are not hit by it directly. This phenomenon is known as the bystander effect, where response molecules pass from tumor cells to normal ones, through communication channels called gap junctions. The use of the enhanced permeability and retention effect, through which drug-loaded nanoparticles smaller than 200 nm may accumulate around a tumor, can prevent the local side effect upon controlled release of the drug. The present work, aimed at functionalizing MCM-41 (Mobil Composition of Matter No. 41) silica nanoparticles with FFA and determining its biocompatibility with human fibroblasts MRC-5 (Medical Research Council cell strain 5). MCM-41, was synthesized and characterized structurally and chemically, with multiple techniques. The biocompatibility assay was performed by Live/Dead technique, with calcein and propidium–iodide. MRC-5 cells were treated with FFA-grafted MCM-41 for 48 h, and 98% of cells remained viable, without signs of necrosis or morphological changes. The results show the feasibility of MCM-41 functionalization with FFA, and its potential protection of normal cells, in comparison to the role of FFA in cancerous ones. MDPI 2018-01-09 /pmc/articles/PMC5874870/ /pubmed/29315235 http://dx.doi.org/10.3390/bioengineering5010004 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Lara, Giovanna Gomes Cipreste, Marcelo Fernandes Andrade, Gracielle Ferreira da Silva, Wellington Marcos de Sousa, Edésia Martins Barros Response of Fibroblasts MRC-5 to Flufenamic Acid-Grafted MCM-41 Nanoparticles |
title | Response of Fibroblasts MRC-5 to Flufenamic Acid-Grafted MCM-41 Nanoparticles |
title_full | Response of Fibroblasts MRC-5 to Flufenamic Acid-Grafted MCM-41 Nanoparticles |
title_fullStr | Response of Fibroblasts MRC-5 to Flufenamic Acid-Grafted MCM-41 Nanoparticles |
title_full_unstemmed | Response of Fibroblasts MRC-5 to Flufenamic Acid-Grafted MCM-41 Nanoparticles |
title_short | Response of Fibroblasts MRC-5 to Flufenamic Acid-Grafted MCM-41 Nanoparticles |
title_sort | response of fibroblasts mrc-5 to flufenamic acid-grafted mcm-41 nanoparticles |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5874870/ https://www.ncbi.nlm.nih.gov/pubmed/29315235 http://dx.doi.org/10.3390/bioengineering5010004 |
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