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Concurrent Host-Pathogen Transcriptional Responses in a Clostridium perfringens Murine Myonecrosis Infection
To obtain an insight into host-pathogen interactions in clostridial myonecrosis, we carried out comparative transcriptome analysis of both the bacterium and the host in a murine Clostridium perfringens infection model, which is the first time that such an investigation has been conducted. Analysis o...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Society for Microbiology
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5874911/ https://www.ncbi.nlm.nih.gov/pubmed/29588405 http://dx.doi.org/10.1128/mBio.00473-18 |
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author | Low, Lee-Yean Harrison, Paul F. Gould, Jodee Powell, David R. Choo, Jocelyn M. Forster, Samuel C. Chapman, Ross Gearing, Linden J. Cheung, Jackie K. Hertzog, Paul Rood, Julian I. |
author_facet | Low, Lee-Yean Harrison, Paul F. Gould, Jodee Powell, David R. Choo, Jocelyn M. Forster, Samuel C. Chapman, Ross Gearing, Linden J. Cheung, Jackie K. Hertzog, Paul Rood, Julian I. |
author_sort | Low, Lee-Yean |
collection | PubMed |
description | To obtain an insight into host-pathogen interactions in clostridial myonecrosis, we carried out comparative transcriptome analysis of both the bacterium and the host in a murine Clostridium perfringens infection model, which is the first time that such an investigation has been conducted. Analysis of the host transcriptome from infected muscle tissues indicated that many genes were upregulated compared to the results seen with mock-infected mice. These genes were enriched for host defense pathways, including Toll-like receptor (TLR) and Nod-like receptor (NLR) signaling components. Real-time PCR confirmed that host TLR2 and NLRP3 inflammasome genes were induced in response to C. perfringens infection. Comparison of the transcriptome of C. perfringens cells from the infected tissues with that from broth cultures showed that host selective pressure induced a global change in C. perfringens gene expression. A total of 33% (923) of C. perfringens genes were differentially regulated, including 10 potential virulence genes that were upregulated relative to their expression in vitro. These genes encoded putative proteins that may be involved in the synthesis of cell wall-associated macromolecules, in adhesion to host cells, or in protection from host cationic antimicrobial peptides. This report presents the first successful expression profiling of coregulated transcriptomes of bacterial and host genes during a clostridial myonecrosis infection and provides new insights into disease pathogenesis and host-pathogen interactions. |
format | Online Article Text |
id | pubmed-5874911 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | American Society for Microbiology |
record_format | MEDLINE/PubMed |
spelling | pubmed-58749112018-03-29 Concurrent Host-Pathogen Transcriptional Responses in a Clostridium perfringens Murine Myonecrosis Infection Low, Lee-Yean Harrison, Paul F. Gould, Jodee Powell, David R. Choo, Jocelyn M. Forster, Samuel C. Chapman, Ross Gearing, Linden J. Cheung, Jackie K. Hertzog, Paul Rood, Julian I. mBio Research Article To obtain an insight into host-pathogen interactions in clostridial myonecrosis, we carried out comparative transcriptome analysis of both the bacterium and the host in a murine Clostridium perfringens infection model, which is the first time that such an investigation has been conducted. Analysis of the host transcriptome from infected muscle tissues indicated that many genes were upregulated compared to the results seen with mock-infected mice. These genes were enriched for host defense pathways, including Toll-like receptor (TLR) and Nod-like receptor (NLR) signaling components. Real-time PCR confirmed that host TLR2 and NLRP3 inflammasome genes were induced in response to C. perfringens infection. Comparison of the transcriptome of C. perfringens cells from the infected tissues with that from broth cultures showed that host selective pressure induced a global change in C. perfringens gene expression. A total of 33% (923) of C. perfringens genes were differentially regulated, including 10 potential virulence genes that were upregulated relative to their expression in vitro. These genes encoded putative proteins that may be involved in the synthesis of cell wall-associated macromolecules, in adhesion to host cells, or in protection from host cationic antimicrobial peptides. This report presents the first successful expression profiling of coregulated transcriptomes of bacterial and host genes during a clostridial myonecrosis infection and provides new insights into disease pathogenesis and host-pathogen interactions. American Society for Microbiology 2018-03-27 /pmc/articles/PMC5874911/ /pubmed/29588405 http://dx.doi.org/10.1128/mBio.00473-18 Text en Copyright © 2018 Low et al. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Research Article Low, Lee-Yean Harrison, Paul F. Gould, Jodee Powell, David R. Choo, Jocelyn M. Forster, Samuel C. Chapman, Ross Gearing, Linden J. Cheung, Jackie K. Hertzog, Paul Rood, Julian I. Concurrent Host-Pathogen Transcriptional Responses in a Clostridium perfringens Murine Myonecrosis Infection |
title | Concurrent Host-Pathogen Transcriptional Responses in a Clostridium perfringens Murine Myonecrosis Infection |
title_full | Concurrent Host-Pathogen Transcriptional Responses in a Clostridium perfringens Murine Myonecrosis Infection |
title_fullStr | Concurrent Host-Pathogen Transcriptional Responses in a Clostridium perfringens Murine Myonecrosis Infection |
title_full_unstemmed | Concurrent Host-Pathogen Transcriptional Responses in a Clostridium perfringens Murine Myonecrosis Infection |
title_short | Concurrent Host-Pathogen Transcriptional Responses in a Clostridium perfringens Murine Myonecrosis Infection |
title_sort | concurrent host-pathogen transcriptional responses in a clostridium perfringens murine myonecrosis infection |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5874911/ https://www.ncbi.nlm.nih.gov/pubmed/29588405 http://dx.doi.org/10.1128/mBio.00473-18 |
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