Metabolic profiling of follistatin overexpression: a novel therapeutic strategy for metabolic diseases

BACKGROUND: Follistatin (Fst) promotes brown adipocyte characteristics in adipose tissues. METHODS: Abdominal fat volume (CT scan), glucose clearance (GTT test), and metabolomics analysis (mass spectrometry) of adipose tissues from Fst transgenic (Fst-Tg) and wild type (WT) control mice were analyze...

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Autores principales: Singh, Rajan, Pervin, Shehla, Lee, Se-Jin, Kuo, Alan, Grijalva, Victor, David, John, Vergnes, Laurent, Reddy, Srinivasa T
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Dove Medical Press 2018
Materias:
Original Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5875402/
https://www.ncbi.nlm.nih.gov/pubmed/29618935
http://dx.doi.org/10.2147/DMSO.S159315
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author Singh, Rajan
Pervin, Shehla
Lee, Se-Jin
Kuo, Alan
Grijalva, Victor
David, John
Vergnes, Laurent
Reddy, Srinivasa T
author_facet Singh, Rajan
Pervin, Shehla
Lee, Se-Jin
Kuo, Alan
Grijalva, Victor
David, John
Vergnes, Laurent
Reddy, Srinivasa T
author_sort Singh, Rajan
collection PubMed
description BACKGROUND: Follistatin (Fst) promotes brown adipocyte characteristics in adipose tissues. METHODS: Abdominal fat volume (CT scan), glucose clearance (GTT test), and metabolomics analysis (mass spectrometry) of adipose tissues from Fst transgenic (Fst-Tg) and wild type (WT) control mice were analyzed. Oxygen consumption (Seahorse Analyzer) and lipidomics (gas chromatography) was analyzed in 3T3-L1 cells. RESULTS: Fst-Tg mice show significant decrease in abdominal fat content, increased glucose clearance, improved plasma lipid profiles and significant changes in several conventional metabolites compared to the WT mice. Furthermore, overexpression of Fst in 3T3-L1 cells resulted in up regulation of key brown/beige markers and changes in lipidomics profiles. CONCLUSION: Fst modulates key factors involved in promoting metabolic syndrome and could be used for therapeutic intervention.
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spelling pubmed-58754022018-04-04 Metabolic profiling of follistatin overexpression: a novel therapeutic strategy for metabolic diseases Singh, Rajan Pervin, Shehla Lee, Se-Jin Kuo, Alan Grijalva, Victor David, John Vergnes, Laurent Reddy, Srinivasa T Diabetes Metab Syndr Obes Original Research BACKGROUND: Follistatin (Fst) promotes brown adipocyte characteristics in adipose tissues. METHODS: Abdominal fat volume (CT scan), glucose clearance (GTT test), and metabolomics analysis (mass spectrometry) of adipose tissues from Fst transgenic (Fst-Tg) and wild type (WT) control mice were analyzed. Oxygen consumption (Seahorse Analyzer) and lipidomics (gas chromatography) was analyzed in 3T3-L1 cells. RESULTS: Fst-Tg mice show significant decrease in abdominal fat content, increased glucose clearance, improved plasma lipid profiles and significant changes in several conventional metabolites compared to the WT mice. Furthermore, overexpression of Fst in 3T3-L1 cells resulted in up regulation of key brown/beige markers and changes in lipidomics profiles. CONCLUSION: Fst modulates key factors involved in promoting metabolic syndrome and could be used for therapeutic intervention. Dove Medical Press 2018-03-26 /pmc/articles/PMC5875402/ /pubmed/29618935 http://dx.doi.org/10.2147/DMSO.S159315 Text en © 2018 Singh et al. This work is published and licensed by Dove Medical Press Limited The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed.
spellingShingle Original Research
Singh, Rajan
Pervin, Shehla
Lee, Se-Jin
Kuo, Alan
Grijalva, Victor
David, John
Vergnes, Laurent
Reddy, Srinivasa T
Metabolic profiling of follistatin overexpression: a novel therapeutic strategy for metabolic diseases
title Metabolic profiling of follistatin overexpression: a novel therapeutic strategy for metabolic diseases
title_full Metabolic profiling of follistatin overexpression: a novel therapeutic strategy for metabolic diseases
title_fullStr Metabolic profiling of follistatin overexpression: a novel therapeutic strategy for metabolic diseases
title_full_unstemmed Metabolic profiling of follistatin overexpression: a novel therapeutic strategy for metabolic diseases
title_short Metabolic profiling of follistatin overexpression: a novel therapeutic strategy for metabolic diseases
title_sort metabolic profiling of follistatin overexpression: a novel therapeutic strategy for metabolic diseases
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5875402/
https://www.ncbi.nlm.nih.gov/pubmed/29618935
http://dx.doi.org/10.2147/DMSO.S159315
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