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Imaging Neuronal Activity in the Optic Tectum of Late Stage Larval Zebrafish

The zebrafish is an established model to study the development and function of visual neuronal circuits in vivo, largely due to their optical accessibility at embryonic and larval stages. In the past decade multiple experimental paradigms have been developed to study visually-driven behaviours, part...

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Autores principales: Bergmann, Katharina, Meza Santoscoy, Paola, Lygdas, Konstantinos, Nikolaeva, Yulia, MacDonald, Ryan B., Cunliffe, Vincent T., Nikolaev, Anton
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5875565/
https://www.ncbi.nlm.nih.gov/pubmed/29615555
http://dx.doi.org/10.3390/jdb6010006
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author Bergmann, Katharina
Meza Santoscoy, Paola
Lygdas, Konstantinos
Nikolaeva, Yulia
MacDonald, Ryan B.
Cunliffe, Vincent T.
Nikolaev, Anton
author_facet Bergmann, Katharina
Meza Santoscoy, Paola
Lygdas, Konstantinos
Nikolaeva, Yulia
MacDonald, Ryan B.
Cunliffe, Vincent T.
Nikolaev, Anton
author_sort Bergmann, Katharina
collection PubMed
description The zebrafish is an established model to study the development and function of visual neuronal circuits in vivo, largely due to their optical accessibility at embryonic and larval stages. In the past decade multiple experimental paradigms have been developed to study visually-driven behaviours, particularly those regulated by the optic tectum, the main visual centre in lower vertebrates. With few exceptions these techniques are limited to young larvae (7–9 days post-fertilisation, dpf). However, many forms of visually-driven behaviour, such as shoaling, emerge at later developmental stages. Consequently, there is a need for an experimental paradigm to image the visual system in zebrafish larvae beyond 9 dpf. Here, we show that using NBT:GCaMP3 line allows for imaging neuronal activity in the optic tectum in late stage larvae until at least 21 dpf. Utilising this line, we have characterised the receptive field properties of tectal neurons of the 2–3 weeks old fish in the cell bodies and the neuropil. The NBT:GCaMP3 line provides a complementary approach and additional opportunities to study neuronal activity in late stage zebrafish larvae.
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spelling pubmed-58755652018-03-30 Imaging Neuronal Activity in the Optic Tectum of Late Stage Larval Zebrafish Bergmann, Katharina Meza Santoscoy, Paola Lygdas, Konstantinos Nikolaeva, Yulia MacDonald, Ryan B. Cunliffe, Vincent T. Nikolaev, Anton J Dev Biol Article The zebrafish is an established model to study the development and function of visual neuronal circuits in vivo, largely due to their optical accessibility at embryonic and larval stages. In the past decade multiple experimental paradigms have been developed to study visually-driven behaviours, particularly those regulated by the optic tectum, the main visual centre in lower vertebrates. With few exceptions these techniques are limited to young larvae (7–9 days post-fertilisation, dpf). However, many forms of visually-driven behaviour, such as shoaling, emerge at later developmental stages. Consequently, there is a need for an experimental paradigm to image the visual system in zebrafish larvae beyond 9 dpf. Here, we show that using NBT:GCaMP3 line allows for imaging neuronal activity in the optic tectum in late stage larvae until at least 21 dpf. Utilising this line, we have characterised the receptive field properties of tectal neurons of the 2–3 weeks old fish in the cell bodies and the neuropil. The NBT:GCaMP3 line provides a complementary approach and additional opportunities to study neuronal activity in late stage zebrafish larvae. MDPI 2018-03-09 /pmc/articles/PMC5875565/ /pubmed/29615555 http://dx.doi.org/10.3390/jdb6010006 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bergmann, Katharina
Meza Santoscoy, Paola
Lygdas, Konstantinos
Nikolaeva, Yulia
MacDonald, Ryan B.
Cunliffe, Vincent T.
Nikolaev, Anton
Imaging Neuronal Activity in the Optic Tectum of Late Stage Larval Zebrafish
title Imaging Neuronal Activity in the Optic Tectum of Late Stage Larval Zebrafish
title_full Imaging Neuronal Activity in the Optic Tectum of Late Stage Larval Zebrafish
title_fullStr Imaging Neuronal Activity in the Optic Tectum of Late Stage Larval Zebrafish
title_full_unstemmed Imaging Neuronal Activity in the Optic Tectum of Late Stage Larval Zebrafish
title_short Imaging Neuronal Activity in the Optic Tectum of Late Stage Larval Zebrafish
title_sort imaging neuronal activity in the optic tectum of late stage larval zebrafish
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5875565/
https://www.ncbi.nlm.nih.gov/pubmed/29615555
http://dx.doi.org/10.3390/jdb6010006
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