Cargando…
Response of human normal and leukemia cells to factors released by amnion fragments in vitro
Amnion is a membrane surrounding the embryo/fetus which determine growth factors and interleukins with angiogenic, immunogenic, and anti-inflammatory properties. The aim of the present study was to investigate the effects of conditioned culture medium from 24-h cultures of human amnion (hAM CCM) on...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5875856/ https://www.ncbi.nlm.nih.gov/pubmed/29596519 http://dx.doi.org/10.1371/journal.pone.0195035 |
_version_ | 1783310427272249344 |
---|---|
author | Grzywocz, Zofia Hoser, Grazyna Sabalinska, Stanislawa Ladyzynski, Piotr Czubak, Jaroslaw Dworczynska, Malgorzata Debski, Romuald Pius-Sadowska, Ewa Machalinski, Boguslaw Kawiak, Jerzy |
author_facet | Grzywocz, Zofia Hoser, Grazyna Sabalinska, Stanislawa Ladyzynski, Piotr Czubak, Jaroslaw Dworczynska, Malgorzata Debski, Romuald Pius-Sadowska, Ewa Machalinski, Boguslaw Kawiak, Jerzy |
author_sort | Grzywocz, Zofia |
collection | PubMed |
description | Amnion is a membrane surrounding the embryo/fetus which determine growth factors and interleukins with angiogenic, immunogenic, and anti-inflammatory properties. The aim of the present study was to investigate the effects of conditioned culture medium from 24-h cultures of human amnion (hAM CCM) on migration and proliferation of human umbilical vein endothelial primary cells (HUVECs), freshly isolated bone marrow mononuclear cells (BM MNCs), and Jurkat leukemia cell line. Amnion membrane was freshly isolated from healthy placenta and its fragments cultured in vitro to produce hAM CCM. Members of the IGFBP protein family made up one third of all assayed proteins present in the hAM medium. The hAM CCM did not affect the proliferation rate of HUVECs or MNCs, but we observed more intensive migration of those cells, and lower expression of CD31 surface antigen on HUVECs as compared to control cultures. In contrast, Jurkat cells did not respond to hAM CCM treatment by proliferation or mobility change. The conditioned medium from 24-h cultures of human amnion is easy to obtain and is a convenient source of various growth and other factors that may be useful in practical medicine. |
format | Online Article Text |
id | pubmed-5875856 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-58758562018-04-13 Response of human normal and leukemia cells to factors released by amnion fragments in vitro Grzywocz, Zofia Hoser, Grazyna Sabalinska, Stanislawa Ladyzynski, Piotr Czubak, Jaroslaw Dworczynska, Malgorzata Debski, Romuald Pius-Sadowska, Ewa Machalinski, Boguslaw Kawiak, Jerzy PLoS One Research Article Amnion is a membrane surrounding the embryo/fetus which determine growth factors and interleukins with angiogenic, immunogenic, and anti-inflammatory properties. The aim of the present study was to investigate the effects of conditioned culture medium from 24-h cultures of human amnion (hAM CCM) on migration and proliferation of human umbilical vein endothelial primary cells (HUVECs), freshly isolated bone marrow mononuclear cells (BM MNCs), and Jurkat leukemia cell line. Amnion membrane was freshly isolated from healthy placenta and its fragments cultured in vitro to produce hAM CCM. Members of the IGFBP protein family made up one third of all assayed proteins present in the hAM medium. The hAM CCM did not affect the proliferation rate of HUVECs or MNCs, but we observed more intensive migration of those cells, and lower expression of CD31 surface antigen on HUVECs as compared to control cultures. In contrast, Jurkat cells did not respond to hAM CCM treatment by proliferation or mobility change. The conditioned medium from 24-h cultures of human amnion is easy to obtain and is a convenient source of various growth and other factors that may be useful in practical medicine. Public Library of Science 2018-03-29 /pmc/articles/PMC5875856/ /pubmed/29596519 http://dx.doi.org/10.1371/journal.pone.0195035 Text en © 2018 Grzywocz et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Grzywocz, Zofia Hoser, Grazyna Sabalinska, Stanislawa Ladyzynski, Piotr Czubak, Jaroslaw Dworczynska, Malgorzata Debski, Romuald Pius-Sadowska, Ewa Machalinski, Boguslaw Kawiak, Jerzy Response of human normal and leukemia cells to factors released by amnion fragments in vitro |
title | Response of human normal and leukemia cells to factors released by amnion fragments in vitro |
title_full | Response of human normal and leukemia cells to factors released by amnion fragments in vitro |
title_fullStr | Response of human normal and leukemia cells to factors released by amnion fragments in vitro |
title_full_unstemmed | Response of human normal and leukemia cells to factors released by amnion fragments in vitro |
title_short | Response of human normal and leukemia cells to factors released by amnion fragments in vitro |
title_sort | response of human normal and leukemia cells to factors released by amnion fragments in vitro |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5875856/ https://www.ncbi.nlm.nih.gov/pubmed/29596519 http://dx.doi.org/10.1371/journal.pone.0195035 |
work_keys_str_mv | AT grzywoczzofia responseofhumannormalandleukemiacellstofactorsreleasedbyamnionfragmentsinvitro AT hosergrazyna responseofhumannormalandleukemiacellstofactorsreleasedbyamnionfragmentsinvitro AT sabalinskastanislawa responseofhumannormalandleukemiacellstofactorsreleasedbyamnionfragmentsinvitro AT ladyzynskipiotr responseofhumannormalandleukemiacellstofactorsreleasedbyamnionfragmentsinvitro AT czubakjaroslaw responseofhumannormalandleukemiacellstofactorsreleasedbyamnionfragmentsinvitro AT dworczynskamalgorzata responseofhumannormalandleukemiacellstofactorsreleasedbyamnionfragmentsinvitro AT debskiromuald responseofhumannormalandleukemiacellstofactorsreleasedbyamnionfragmentsinvitro AT piussadowskaewa responseofhumannormalandleukemiacellstofactorsreleasedbyamnionfragmentsinvitro AT machalinskiboguslaw responseofhumannormalandleukemiacellstofactorsreleasedbyamnionfragmentsinvitro AT kawiakjerzy responseofhumannormalandleukemiacellstofactorsreleasedbyamnionfragmentsinvitro |