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Response of human normal and leukemia cells to factors released by amnion fragments in vitro

Amnion is a membrane surrounding the embryo/fetus which determine growth factors and interleukins with angiogenic, immunogenic, and anti-inflammatory properties. The aim of the present study was to investigate the effects of conditioned culture medium from 24-h cultures of human amnion (hAM CCM) on...

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Autores principales: Grzywocz, Zofia, Hoser, Grazyna, Sabalinska, Stanislawa, Ladyzynski, Piotr, Czubak, Jaroslaw, Dworczynska, Malgorzata, Debski, Romuald, Pius-Sadowska, Ewa, Machalinski, Boguslaw, Kawiak, Jerzy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5875856/
https://www.ncbi.nlm.nih.gov/pubmed/29596519
http://dx.doi.org/10.1371/journal.pone.0195035
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author Grzywocz, Zofia
Hoser, Grazyna
Sabalinska, Stanislawa
Ladyzynski, Piotr
Czubak, Jaroslaw
Dworczynska, Malgorzata
Debski, Romuald
Pius-Sadowska, Ewa
Machalinski, Boguslaw
Kawiak, Jerzy
author_facet Grzywocz, Zofia
Hoser, Grazyna
Sabalinska, Stanislawa
Ladyzynski, Piotr
Czubak, Jaroslaw
Dworczynska, Malgorzata
Debski, Romuald
Pius-Sadowska, Ewa
Machalinski, Boguslaw
Kawiak, Jerzy
author_sort Grzywocz, Zofia
collection PubMed
description Amnion is a membrane surrounding the embryo/fetus which determine growth factors and interleukins with angiogenic, immunogenic, and anti-inflammatory properties. The aim of the present study was to investigate the effects of conditioned culture medium from 24-h cultures of human amnion (hAM CCM) on migration and proliferation of human umbilical vein endothelial primary cells (HUVECs), freshly isolated bone marrow mononuclear cells (BM MNCs), and Jurkat leukemia cell line. Amnion membrane was freshly isolated from healthy placenta and its fragments cultured in vitro to produce hAM CCM. Members of the IGFBP protein family made up one third of all assayed proteins present in the hAM medium. The hAM CCM did not affect the proliferation rate of HUVECs or MNCs, but we observed more intensive migration of those cells, and lower expression of CD31 surface antigen on HUVECs as compared to control cultures. In contrast, Jurkat cells did not respond to hAM CCM treatment by proliferation or mobility change. The conditioned medium from 24-h cultures of human amnion is easy to obtain and is a convenient source of various growth and other factors that may be useful in practical medicine.
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spelling pubmed-58758562018-04-13 Response of human normal and leukemia cells to factors released by amnion fragments in vitro Grzywocz, Zofia Hoser, Grazyna Sabalinska, Stanislawa Ladyzynski, Piotr Czubak, Jaroslaw Dworczynska, Malgorzata Debski, Romuald Pius-Sadowska, Ewa Machalinski, Boguslaw Kawiak, Jerzy PLoS One Research Article Amnion is a membrane surrounding the embryo/fetus which determine growth factors and interleukins with angiogenic, immunogenic, and anti-inflammatory properties. The aim of the present study was to investigate the effects of conditioned culture medium from 24-h cultures of human amnion (hAM CCM) on migration and proliferation of human umbilical vein endothelial primary cells (HUVECs), freshly isolated bone marrow mononuclear cells (BM MNCs), and Jurkat leukemia cell line. Amnion membrane was freshly isolated from healthy placenta and its fragments cultured in vitro to produce hAM CCM. Members of the IGFBP protein family made up one third of all assayed proteins present in the hAM medium. The hAM CCM did not affect the proliferation rate of HUVECs or MNCs, but we observed more intensive migration of those cells, and lower expression of CD31 surface antigen on HUVECs as compared to control cultures. In contrast, Jurkat cells did not respond to hAM CCM treatment by proliferation or mobility change. The conditioned medium from 24-h cultures of human amnion is easy to obtain and is a convenient source of various growth and other factors that may be useful in practical medicine. Public Library of Science 2018-03-29 /pmc/articles/PMC5875856/ /pubmed/29596519 http://dx.doi.org/10.1371/journal.pone.0195035 Text en © 2018 Grzywocz et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Grzywocz, Zofia
Hoser, Grazyna
Sabalinska, Stanislawa
Ladyzynski, Piotr
Czubak, Jaroslaw
Dworczynska, Malgorzata
Debski, Romuald
Pius-Sadowska, Ewa
Machalinski, Boguslaw
Kawiak, Jerzy
Response of human normal and leukemia cells to factors released by amnion fragments in vitro
title Response of human normal and leukemia cells to factors released by amnion fragments in vitro
title_full Response of human normal and leukemia cells to factors released by amnion fragments in vitro
title_fullStr Response of human normal and leukemia cells to factors released by amnion fragments in vitro
title_full_unstemmed Response of human normal and leukemia cells to factors released by amnion fragments in vitro
title_short Response of human normal and leukemia cells to factors released by amnion fragments in vitro
title_sort response of human normal and leukemia cells to factors released by amnion fragments in vitro
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5875856/
https://www.ncbi.nlm.nih.gov/pubmed/29596519
http://dx.doi.org/10.1371/journal.pone.0195035
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