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Single-cell qPCR facilitates the optimization of hematopoietic differentiation in hPSCs/OP9 coculture system
Human pluripotent stem cells (hPSCs)/OP9 coculture system is a widely used hematopoietic differentiation approach. The limited understanding of this process leads to its low efficiency. Thus, we used single-cell qPCR to reveal the gene expression profiles of individual CD34(+) cells from different s...
Autores principales: | Chen, Haide, Jiang, Mengmeng, Xiao, Lei, Huang, He |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Associação Brasileira de Divulgação Científica
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5875903/ https://www.ncbi.nlm.nih.gov/pubmed/29561959 http://dx.doi.org/10.1590/1414-431X20187183 |
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