Cargando…
A Statistically Representative Atlas for Mapping Neuronal Circuits in the Drosophila Adult Brain
Imaging the expression patterns of reporter constructs is a powerful tool to dissect the neuronal circuits of perception and behavior in the adult brain of Drosophila, one of the major models for studying brain functions. To date, several Drosophila brain templates and digital atlases have been buil...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5876320/ https://www.ncbi.nlm.nih.gov/pubmed/29628885 http://dx.doi.org/10.3389/fninf.2018.00013 |
_version_ | 1783310487715315712 |
---|---|
author | Arganda-Carreras, Ignacio Manoliu, Tudor Mazuras, Nicolas Schulze, Florian Iglesias, Juan E. Bühler, Katja Jenett, Arnim Rouyer, François Andrey, Philippe |
author_facet | Arganda-Carreras, Ignacio Manoliu, Tudor Mazuras, Nicolas Schulze, Florian Iglesias, Juan E. Bühler, Katja Jenett, Arnim Rouyer, François Andrey, Philippe |
author_sort | Arganda-Carreras, Ignacio |
collection | PubMed |
description | Imaging the expression patterns of reporter constructs is a powerful tool to dissect the neuronal circuits of perception and behavior in the adult brain of Drosophila, one of the major models for studying brain functions. To date, several Drosophila brain templates and digital atlases have been built to automatically analyze and compare collections of expression pattern images. However, there has been no systematic comparison of performances between alternative atlasing strategies and registration algorithms. Here, we objectively evaluated the performance of different strategies for building adult Drosophila brain templates and atlases. In addition, we used state-of-the-art registration algorithms to generate a new group-wise inter-sex atlas. Our results highlight the benefit of statistical atlases over individual ones and show that the newly proposed inter-sex atlas outperformed existing solutions for automated registration and annotation of expression patterns. Over 3,000 images from the Janelia Farm FlyLight collection were registered using the proposed strategy. These registered expression patterns can be searched and compared with a new version of the BrainBaseWeb system and BrainGazer software. We illustrate the validity of our methodology and brain atlas with registration-based predictions of expression patterns in a subset of clock neurons. The described registration framework should benefit to brain studies in Drosophila and other insect species. |
format | Online Article Text |
id | pubmed-5876320 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-58763202018-04-06 A Statistically Representative Atlas for Mapping Neuronal Circuits in the Drosophila Adult Brain Arganda-Carreras, Ignacio Manoliu, Tudor Mazuras, Nicolas Schulze, Florian Iglesias, Juan E. Bühler, Katja Jenett, Arnim Rouyer, François Andrey, Philippe Front Neuroinform Neuroscience Imaging the expression patterns of reporter constructs is a powerful tool to dissect the neuronal circuits of perception and behavior in the adult brain of Drosophila, one of the major models for studying brain functions. To date, several Drosophila brain templates and digital atlases have been built to automatically analyze and compare collections of expression pattern images. However, there has been no systematic comparison of performances between alternative atlasing strategies and registration algorithms. Here, we objectively evaluated the performance of different strategies for building adult Drosophila brain templates and atlases. In addition, we used state-of-the-art registration algorithms to generate a new group-wise inter-sex atlas. Our results highlight the benefit of statistical atlases over individual ones and show that the newly proposed inter-sex atlas outperformed existing solutions for automated registration and annotation of expression patterns. Over 3,000 images from the Janelia Farm FlyLight collection were registered using the proposed strategy. These registered expression patterns can be searched and compared with a new version of the BrainBaseWeb system and BrainGazer software. We illustrate the validity of our methodology and brain atlas with registration-based predictions of expression patterns in a subset of clock neurons. The described registration framework should benefit to brain studies in Drosophila and other insect species. Frontiers Media S.A. 2018-03-23 /pmc/articles/PMC5876320/ /pubmed/29628885 http://dx.doi.org/10.3389/fninf.2018.00013 Text en Copyright © 2018 Arganda-Carreras, Manoliu, Mazuras, Schulze, Iglesias, Bühler, Jenett, Rouyer and Andrey. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Arganda-Carreras, Ignacio Manoliu, Tudor Mazuras, Nicolas Schulze, Florian Iglesias, Juan E. Bühler, Katja Jenett, Arnim Rouyer, François Andrey, Philippe A Statistically Representative Atlas for Mapping Neuronal Circuits in the Drosophila Adult Brain |
title | A Statistically Representative Atlas for Mapping Neuronal Circuits in the Drosophila Adult Brain |
title_full | A Statistically Representative Atlas for Mapping Neuronal Circuits in the Drosophila Adult Brain |
title_fullStr | A Statistically Representative Atlas for Mapping Neuronal Circuits in the Drosophila Adult Brain |
title_full_unstemmed | A Statistically Representative Atlas for Mapping Neuronal Circuits in the Drosophila Adult Brain |
title_short | A Statistically Representative Atlas for Mapping Neuronal Circuits in the Drosophila Adult Brain |
title_sort | statistically representative atlas for mapping neuronal circuits in the drosophila adult brain |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5876320/ https://www.ncbi.nlm.nih.gov/pubmed/29628885 http://dx.doi.org/10.3389/fninf.2018.00013 |
work_keys_str_mv | AT argandacarrerasignacio astatisticallyrepresentativeatlasformappingneuronalcircuitsinthedrosophilaadultbrain AT manoliutudor astatisticallyrepresentativeatlasformappingneuronalcircuitsinthedrosophilaadultbrain AT mazurasnicolas astatisticallyrepresentativeatlasformappingneuronalcircuitsinthedrosophilaadultbrain AT schulzeflorian astatisticallyrepresentativeatlasformappingneuronalcircuitsinthedrosophilaadultbrain AT iglesiasjuane astatisticallyrepresentativeatlasformappingneuronalcircuitsinthedrosophilaadultbrain AT buhlerkatja astatisticallyrepresentativeatlasformappingneuronalcircuitsinthedrosophilaadultbrain AT jenettarnim astatisticallyrepresentativeatlasformappingneuronalcircuitsinthedrosophilaadultbrain AT rouyerfrancois astatisticallyrepresentativeatlasformappingneuronalcircuitsinthedrosophilaadultbrain AT andreyphilippe astatisticallyrepresentativeatlasformappingneuronalcircuitsinthedrosophilaadultbrain AT argandacarrerasignacio statisticallyrepresentativeatlasformappingneuronalcircuitsinthedrosophilaadultbrain AT manoliutudor statisticallyrepresentativeatlasformappingneuronalcircuitsinthedrosophilaadultbrain AT mazurasnicolas statisticallyrepresentativeatlasformappingneuronalcircuitsinthedrosophilaadultbrain AT schulzeflorian statisticallyrepresentativeatlasformappingneuronalcircuitsinthedrosophilaadultbrain AT iglesiasjuane statisticallyrepresentativeatlasformappingneuronalcircuitsinthedrosophilaadultbrain AT buhlerkatja statisticallyrepresentativeatlasformappingneuronalcircuitsinthedrosophilaadultbrain AT jenettarnim statisticallyrepresentativeatlasformappingneuronalcircuitsinthedrosophilaadultbrain AT rouyerfrancois statisticallyrepresentativeatlasformappingneuronalcircuitsinthedrosophilaadultbrain AT andreyphilippe statisticallyrepresentativeatlasformappingneuronalcircuitsinthedrosophilaadultbrain |