Evaluation of Screened Lignin-degrading Fungi for the Biological Pretreatment of Corn Stover

The biological pretreatment of lignocellulosic biomass is a low-cost and eco-friendly method for facilitating enzymatic hydrolysis. In this study, strains with lignin depletion capability were screened using a high-throughput screening method. Sixty-three strains were screened out and Myrothecium ve...

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Autores principales: Su, Yingjie, Yu, Xiaoxiao, Sun, Yang, Wang, Gang, Chen, Huan, Chen, Guang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5876370/
https://www.ncbi.nlm.nih.gov/pubmed/29599465
http://dx.doi.org/10.1038/s41598-018-23626-6
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author Su, Yingjie
Yu, Xiaoxiao
Sun, Yang
Wang, Gang
Chen, Huan
Chen, Guang
author_facet Su, Yingjie
Yu, Xiaoxiao
Sun, Yang
Wang, Gang
Chen, Huan
Chen, Guang
author_sort Su, Yingjie
collection PubMed
description The biological pretreatment of lignocellulosic biomass is a low-cost and eco-friendly method for facilitating enzymatic hydrolysis. In this study, strains with lignin depletion capability were screened using a high-throughput screening method. Sixty-three strains were screened out and Myrothecium verrucaria secreted three lignin-degrading enzymes simultaneously during the bio-pretreatment process. The activity levels of laccase, lignin peroxidase and manganese peroxidase were 6.61, 0.78 and 1.31 U g(−1) dry biomass. The content of lignin in corn stover decreased by 42.30% after bio-pretreatment, and the conversion rate increased by 123.84% during the subsequent saccharification process in comparison with the untreated corn stover. Furthermore, the effects of bio-pretreatment on the structure of corn stover were presented using a scanning electron microscope (SEM), Brunauer-Emmet-Teller (BET), X-ray diffractometer (XRD) and Fourier transform infrared spectroscopy (FTIR). The results showed that M.V. is a promising lignin-degrading fungus. This research demonstrated an efficient pretreatment approach for enhancing the enzymatic saccharification of corn stover.
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spelling pubmed-58763702018-04-02 Evaluation of Screened Lignin-degrading Fungi for the Biological Pretreatment of Corn Stover Su, Yingjie Yu, Xiaoxiao Sun, Yang Wang, Gang Chen, Huan Chen, Guang Sci Rep Article The biological pretreatment of lignocellulosic biomass is a low-cost and eco-friendly method for facilitating enzymatic hydrolysis. In this study, strains with lignin depletion capability were screened using a high-throughput screening method. Sixty-three strains were screened out and Myrothecium verrucaria secreted three lignin-degrading enzymes simultaneously during the bio-pretreatment process. The activity levels of laccase, lignin peroxidase and manganese peroxidase were 6.61, 0.78 and 1.31 U g(−1) dry biomass. The content of lignin in corn stover decreased by 42.30% after bio-pretreatment, and the conversion rate increased by 123.84% during the subsequent saccharification process in comparison with the untreated corn stover. Furthermore, the effects of bio-pretreatment on the structure of corn stover were presented using a scanning electron microscope (SEM), Brunauer-Emmet-Teller (BET), X-ray diffractometer (XRD) and Fourier transform infrared spectroscopy (FTIR). The results showed that M.V. is a promising lignin-degrading fungus. This research demonstrated an efficient pretreatment approach for enhancing the enzymatic saccharification of corn stover. Nature Publishing Group UK 2018-03-29 /pmc/articles/PMC5876370/ /pubmed/29599465 http://dx.doi.org/10.1038/s41598-018-23626-6 Text en © The Author(s) 2018 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Su, Yingjie
Yu, Xiaoxiao
Sun, Yang
Wang, Gang
Chen, Huan
Chen, Guang
Evaluation of Screened Lignin-degrading Fungi for the Biological Pretreatment of Corn Stover
title Evaluation of Screened Lignin-degrading Fungi for the Biological Pretreatment of Corn Stover
title_full Evaluation of Screened Lignin-degrading Fungi for the Biological Pretreatment of Corn Stover
title_fullStr Evaluation of Screened Lignin-degrading Fungi for the Biological Pretreatment of Corn Stover
title_full_unstemmed Evaluation of Screened Lignin-degrading Fungi for the Biological Pretreatment of Corn Stover
title_short Evaluation of Screened Lignin-degrading Fungi for the Biological Pretreatment of Corn Stover
title_sort evaluation of screened lignin-degrading fungi for the biological pretreatment of corn stover
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5876370/
https://www.ncbi.nlm.nih.gov/pubmed/29599465
http://dx.doi.org/10.1038/s41598-018-23626-6
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