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Hydroxycamptothecin mediates antiproliferative effects through apoptosis and autophagy in A549 cells

Hydroxycamptothecin (HCPT) represents a new generation of anticancer drugs, with almost no side effects when used for the treatment of a number of types of cancer. Autophagy is becoming recognized as an important biological mechanism in human cancer, including lung cancer. However, the involvement o...

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Autores principales: Wei, Yanjie, Li, Chenhao, Zhang, Yuan, He, Hailan, Zhang, Guozhi, Hao, Xiaohui, Liu, Heliang, Wang, Hongli, Tian, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5876437/
https://www.ncbi.nlm.nih.gov/pubmed/29616109
http://dx.doi.org/10.3892/ol.2018.8107
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author Wei, Yanjie
Li, Chenhao
Zhang, Yuan
He, Hailan
Zhang, Guozhi
Hao, Xiaohui
Liu, Heliang
Wang, Hongli
Tian, Wei
author_facet Wei, Yanjie
Li, Chenhao
Zhang, Yuan
He, Hailan
Zhang, Guozhi
Hao, Xiaohui
Liu, Heliang
Wang, Hongli
Tian, Wei
author_sort Wei, Yanjie
collection PubMed
description Hydroxycamptothecin (HCPT) represents a new generation of anticancer drugs, with almost no side effects when used for the treatment of a number of types of cancer. Autophagy is becoming recognized as an important biological mechanism in human cancer, including lung cancer. However, the involvement of autophagy in the antiproliferative effects of HCPT on lung cancer remains unclear. In the present study, A549 cells, an accepted model of non-small cell lung cancer (NSCLC) cells, were employed. It was demonstrated that HCPT was able to suppress proliferation and induce apoptosis and autophagy in A549 cells. The molecular mechanism underlying HCPT-induced cell death was attributed to apoptosis and autophagy. Furthermore, it was demonstrated that an autophagy inhibitor, 3-methyladenine, accelerated HCPT-induced cell death in A549 cells. The results of the present study may lead to a deeper understanding of the molecular mechanism by which HCPT regulates NSCLC A549 cells. These results highlight the potential use of autophagy inhibitors in combination with traditional chemotherapy drugs for the treatment of lung cancer.
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spelling pubmed-58764372018-04-03 Hydroxycamptothecin mediates antiproliferative effects through apoptosis and autophagy in A549 cells Wei, Yanjie Li, Chenhao Zhang, Yuan He, Hailan Zhang, Guozhi Hao, Xiaohui Liu, Heliang Wang, Hongli Tian, Wei Oncol Lett Articles Hydroxycamptothecin (HCPT) represents a new generation of anticancer drugs, with almost no side effects when used for the treatment of a number of types of cancer. Autophagy is becoming recognized as an important biological mechanism in human cancer, including lung cancer. However, the involvement of autophagy in the antiproliferative effects of HCPT on lung cancer remains unclear. In the present study, A549 cells, an accepted model of non-small cell lung cancer (NSCLC) cells, were employed. It was demonstrated that HCPT was able to suppress proliferation and induce apoptosis and autophagy in A549 cells. The molecular mechanism underlying HCPT-induced cell death was attributed to apoptosis and autophagy. Furthermore, it was demonstrated that an autophagy inhibitor, 3-methyladenine, accelerated HCPT-induced cell death in A549 cells. The results of the present study may lead to a deeper understanding of the molecular mechanism by which HCPT regulates NSCLC A549 cells. These results highlight the potential use of autophagy inhibitors in combination with traditional chemotherapy drugs for the treatment of lung cancer. D.A. Spandidos 2018-05 2018-02-22 /pmc/articles/PMC5876437/ /pubmed/29616109 http://dx.doi.org/10.3892/ol.2018.8107 Text en Copyright: © Wei et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Wei, Yanjie
Li, Chenhao
Zhang, Yuan
He, Hailan
Zhang, Guozhi
Hao, Xiaohui
Liu, Heliang
Wang, Hongli
Tian, Wei
Hydroxycamptothecin mediates antiproliferative effects through apoptosis and autophagy in A549 cells
title Hydroxycamptothecin mediates antiproliferative effects through apoptosis and autophagy in A549 cells
title_full Hydroxycamptothecin mediates antiproliferative effects through apoptosis and autophagy in A549 cells
title_fullStr Hydroxycamptothecin mediates antiproliferative effects through apoptosis and autophagy in A549 cells
title_full_unstemmed Hydroxycamptothecin mediates antiproliferative effects through apoptosis and autophagy in A549 cells
title_short Hydroxycamptothecin mediates antiproliferative effects through apoptosis and autophagy in A549 cells
title_sort hydroxycamptothecin mediates antiproliferative effects through apoptosis and autophagy in a549 cells
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5876437/
https://www.ncbi.nlm.nih.gov/pubmed/29616109
http://dx.doi.org/10.3892/ol.2018.8107
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