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Biofilm-Forming Potential of Staphylococcus aureus Isolated from Bovine Mastitis in New Zealand

Biofilm formation is of growing concern in human and animal health. However, it is still unclear how biofilms are related to mastitis infections in dairy cattle. In this study, a comparison between two tests for biofilm formation and the association between biofilm and the presence of genes associat...

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Autores principales: Notcovich, Shirli, DeNicolo, Gina, Flint, Steve H., Williamson, Norman B., Gedye, Kristene, Grinberg, Alex, Lopez-Villalobos, Nicolas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5876566/
https://www.ncbi.nlm.nih.gov/pubmed/29351199
http://dx.doi.org/10.3390/vetsci5010008
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author Notcovich, Shirli
DeNicolo, Gina
Flint, Steve H.
Williamson, Norman B.
Gedye, Kristene
Grinberg, Alex
Lopez-Villalobos, Nicolas
author_facet Notcovich, Shirli
DeNicolo, Gina
Flint, Steve H.
Williamson, Norman B.
Gedye, Kristene
Grinberg, Alex
Lopez-Villalobos, Nicolas
author_sort Notcovich, Shirli
collection PubMed
description Biofilm formation is of growing concern in human and animal health. However, it is still unclear how biofilms are related to mastitis infections in dairy cattle. In this study, a comparison between two tests for biofilm formation and the association between biofilm and the presence of genes associated with biofilm formation were investigated for 92 Staphylococcus aureus isolates from intramammary infections. Congo red agar (CRA) and microtitre test assay (MTA) in vitro phenotypic tests were used to evaluate biofilm formation. The presence of icaA, icaD, and bap genes associated with biofilm formation was confirmed using the polymerase chain reaction. Results show that most of the S. aureus isolates, though not possessing one of the biofilm-forming genes, were able to produce biofilms. MTA was more frequently positive in identifying biofilm-forming isolates than CRA.
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spelling pubmed-58765662018-03-30 Biofilm-Forming Potential of Staphylococcus aureus Isolated from Bovine Mastitis in New Zealand Notcovich, Shirli DeNicolo, Gina Flint, Steve H. Williamson, Norman B. Gedye, Kristene Grinberg, Alex Lopez-Villalobos, Nicolas Vet Sci Communication Biofilm formation is of growing concern in human and animal health. However, it is still unclear how biofilms are related to mastitis infections in dairy cattle. In this study, a comparison between two tests for biofilm formation and the association between biofilm and the presence of genes associated with biofilm formation were investigated for 92 Staphylococcus aureus isolates from intramammary infections. Congo red agar (CRA) and microtitre test assay (MTA) in vitro phenotypic tests were used to evaluate biofilm formation. The presence of icaA, icaD, and bap genes associated with biofilm formation was confirmed using the polymerase chain reaction. Results show that most of the S. aureus isolates, though not possessing one of the biofilm-forming genes, were able to produce biofilms. MTA was more frequently positive in identifying biofilm-forming isolates than CRA. MDPI 2018-01-19 /pmc/articles/PMC5876566/ /pubmed/29351199 http://dx.doi.org/10.3390/vetsci5010008 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Notcovich, Shirli
DeNicolo, Gina
Flint, Steve H.
Williamson, Norman B.
Gedye, Kristene
Grinberg, Alex
Lopez-Villalobos, Nicolas
Biofilm-Forming Potential of Staphylococcus aureus Isolated from Bovine Mastitis in New Zealand
title Biofilm-Forming Potential of Staphylococcus aureus Isolated from Bovine Mastitis in New Zealand
title_full Biofilm-Forming Potential of Staphylococcus aureus Isolated from Bovine Mastitis in New Zealand
title_fullStr Biofilm-Forming Potential of Staphylococcus aureus Isolated from Bovine Mastitis in New Zealand
title_full_unstemmed Biofilm-Forming Potential of Staphylococcus aureus Isolated from Bovine Mastitis in New Zealand
title_short Biofilm-Forming Potential of Staphylococcus aureus Isolated from Bovine Mastitis in New Zealand
title_sort biofilm-forming potential of staphylococcus aureus isolated from bovine mastitis in new zealand
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5876566/
https://www.ncbi.nlm.nih.gov/pubmed/29351199
http://dx.doi.org/10.3390/vetsci5010008
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