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Molecular Identification of Eimeria Species in Broiler Chickens in Trinidad, West Indies

Coccidiosis is an intestinal disease of chickens of major economic importance to broiler industries worldwide. Species of coccidia found in chickens include Eimeria acervulina, Eimeria brunetti, Eimeria maxima, Eimeria mitis, Eimeria necatrix, Eimeria praecox, and Eimeria tenella. In recent years, p...

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Autores principales: Brown Jordan, Arianne, Blake, Damer, Beard, Jamila, Beharry, Asha, Serrette, Louanne, Soleyn, Atlyn, Sookhoo, Jamie, Blake, Lemar, Brown, Gabriel, Oura, Christopher
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5876585/
https://www.ncbi.nlm.nih.gov/pubmed/29361806
http://dx.doi.org/10.3390/vetsci5010012
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author Brown Jordan, Arianne
Blake, Damer
Beard, Jamila
Beharry, Asha
Serrette, Louanne
Soleyn, Atlyn
Sookhoo, Jamie
Blake, Lemar
Brown, Gabriel
Oura, Christopher
author_facet Brown Jordan, Arianne
Blake, Damer
Beard, Jamila
Beharry, Asha
Serrette, Louanne
Soleyn, Atlyn
Sookhoo, Jamie
Blake, Lemar
Brown, Gabriel
Oura, Christopher
author_sort Brown Jordan, Arianne
collection PubMed
description Coccidiosis is an intestinal disease of chickens of major economic importance to broiler industries worldwide. Species of coccidia found in chickens include Eimeria acervulina, Eimeria brunetti, Eimeria maxima, Eimeria mitis, Eimeria necatrix, Eimeria praecox, and Eimeria tenella. In recent years, polymerase chain reaction (PCR) has been developed to provide accurate and rapid identification of the seven known Eimeria species of chickens. The aim of this study was to use species-specific real-time PCR (qPCR) to identify which of the seven Eimeria species are present in Trinidad poultry. Seventeen pooled fecal samples were collected from 6 broiler farms (2–5 pens per farm) across Trinidad. Feces were also collected from birds showing clinical signs of coccidiosis in two live bird markets (pluck shops). qPCR revealed the presence of five species of Eimeria (E. acervulina, E. maxima, E. mitis, E. necatrix, and E. tenella), but not E. brunetti or E. praecox. Mixed infections were detected on all broiler farms, and DNA of two highly pathogenic Eimeria species (E. tenella and E. necatrix) was detected in feces taken from clinically sick birds sampled from the two pluck shops.
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spelling pubmed-58765852018-03-30 Molecular Identification of Eimeria Species in Broiler Chickens in Trinidad, West Indies Brown Jordan, Arianne Blake, Damer Beard, Jamila Beharry, Asha Serrette, Louanne Soleyn, Atlyn Sookhoo, Jamie Blake, Lemar Brown, Gabriel Oura, Christopher Vet Sci Article Coccidiosis is an intestinal disease of chickens of major economic importance to broiler industries worldwide. Species of coccidia found in chickens include Eimeria acervulina, Eimeria brunetti, Eimeria maxima, Eimeria mitis, Eimeria necatrix, Eimeria praecox, and Eimeria tenella. In recent years, polymerase chain reaction (PCR) has been developed to provide accurate and rapid identification of the seven known Eimeria species of chickens. The aim of this study was to use species-specific real-time PCR (qPCR) to identify which of the seven Eimeria species are present in Trinidad poultry. Seventeen pooled fecal samples were collected from 6 broiler farms (2–5 pens per farm) across Trinidad. Feces were also collected from birds showing clinical signs of coccidiosis in two live bird markets (pluck shops). qPCR revealed the presence of five species of Eimeria (E. acervulina, E. maxima, E. mitis, E. necatrix, and E. tenella), but not E. brunetti or E. praecox. Mixed infections were detected on all broiler farms, and DNA of two highly pathogenic Eimeria species (E. tenella and E. necatrix) was detected in feces taken from clinically sick birds sampled from the two pluck shops. MDPI 2018-01-22 /pmc/articles/PMC5876585/ /pubmed/29361806 http://dx.doi.org/10.3390/vetsci5010012 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Brown Jordan, Arianne
Blake, Damer
Beard, Jamila
Beharry, Asha
Serrette, Louanne
Soleyn, Atlyn
Sookhoo, Jamie
Blake, Lemar
Brown, Gabriel
Oura, Christopher
Molecular Identification of Eimeria Species in Broiler Chickens in Trinidad, West Indies
title Molecular Identification of Eimeria Species in Broiler Chickens in Trinidad, West Indies
title_full Molecular Identification of Eimeria Species in Broiler Chickens in Trinidad, West Indies
title_fullStr Molecular Identification of Eimeria Species in Broiler Chickens in Trinidad, West Indies
title_full_unstemmed Molecular Identification of Eimeria Species in Broiler Chickens in Trinidad, West Indies
title_short Molecular Identification of Eimeria Species in Broiler Chickens in Trinidad, West Indies
title_sort molecular identification of eimeria species in broiler chickens in trinidad, west indies
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5876585/
https://www.ncbi.nlm.nih.gov/pubmed/29361806
http://dx.doi.org/10.3390/vetsci5010012
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