Detection of Matrix Metalloproteinase Activity by Bioluminescence via Intein-Mediated Biotinylation of Luciferase

We report bioluminescence analysis of matrix metalloproteinase (MMP) activity in biological substances using a surface-bound luciferase probe. Intein-fused luciferase protein enables site-specific biotinylation of luciferase in the presence of N-terminus cysteine-biotin via intein-mediated splicing...

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Detalles Bibliográficos
Autores principales: Nguyen, Duc Long, Kim, Hanim, Kim, Dasom, Lee, Jin Oh, Gye, Myung Chan, Kim, Young-Pil
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5877304/
https://www.ncbi.nlm.nih.gov/pubmed/29543764
http://dx.doi.org/10.3390/s18030875

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5877304/
https://www.ncbi.nlm.nih.gov/pubmed/29543764
http://dx.doi.org/10.3390/s18030875

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