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Detection of Matrix Metalloproteinase Activity by Bioluminescence via Intein-Mediated Biotinylation of Luciferase

We report bioluminescence analysis of matrix metalloproteinase (MMP) activity in biological substances using a surface-bound luciferase probe. Intein-fused luciferase protein enables site-specific biotinylation of luciferase in the presence of N-terminus cysteine-biotin via intein-mediated splicing...

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Detalles Bibliográficos
Autores principales:	Nguyen, Duc Long, Kim, Hanim, Kim, Dasom, Lee, Jin Oh, Gye, Myung Chan, Kim, Young-Pil
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2018
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5877304/ https://www.ncbi.nlm.nih.gov/pubmed/29543764 http://dx.doi.org/10.3390/s18030875

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