Cargando…
Evaluation of Suppressiveness of Soils Exhibiting Soil-Borne Disease Suppression after Long-Term Application of Organic Amendments by the Co-cultivation Method of Pathogenic Fusarium oxysporum and Indigenous Soil Microorganisms
Preventive measures against soil-borne diseases need to be implemented before cultivation because very few countermeasures are available after the development of diseases. Some soils suppress soil-borne diseases despite the presence of a high population density of pathogens. If the suppressiveness o...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI)
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5877344/ https://www.ncbi.nlm.nih.gov/pubmed/29459498 http://dx.doi.org/10.1264/jsme2.ME17072 |
_version_ | 1783310681593872384 |
---|---|
author | Mitsuboshi, Masahiro Kioka, Yuuzou Noguchi, Katsunori Asakawa, Susumu |
author_facet | Mitsuboshi, Masahiro Kioka, Yuuzou Noguchi, Katsunori Asakawa, Susumu |
author_sort | Mitsuboshi, Masahiro |
collection | PubMed |
description | Preventive measures against soil-borne diseases need to be implemented before cultivation because very few countermeasures are available after the development of diseases. Some soils suppress soil-borne diseases despite the presence of a high population density of pathogens. If the suppressiveness of soil against soil-borne diseases may be predicted and diagnosed for crop fields, it may be possible to reduce the labor and cost associated with excessive disinfection practices. We herein evaluated the suppressiveness of soils in fields with the long-term application of organic amendments by examining the growth of pathogenic Fusarium oxysporum co-cultivated with indigenous soil microorganisms on agar plates. Soils treated with coffee residue compost or rapeseed meal showed suppressiveness against spinach wilt disease by F. oxysporum f. sp. spinaciae or spinach wilt and lettuce root rot diseases by F. oxysporum f. sp. spinaciae and F. oxysporum f. sp. lactucae, respectively, and the growth of pathogenic Fusarium spp. on agar plates was suppressed when co-cultured with microorganisms in a suspension from these soils before crop cultivation. These results indicate the potential of the growth degree of pathogenic F. oxysporum estimated by this method as a diagnostic indicator of the suppressiveness of soil associated with the inhabiting microorganisms. A correlation was found between the incidence of spinach wilt disease in spinach and the growth degree of F. oxysporum f. sp. spinaciae by this co-cultivation method, indicating that suppressiveness induced by organic amendment applications against F. oxysporum f. sp. spinaciae is evaluable by this method. The co-cultivation method may be useful for predicting and diagnosing suppressiveness against soil-borne diseases. |
format | Online Article Text |
id | pubmed-5877344 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) |
record_format | MEDLINE/PubMed |
spelling | pubmed-58773442018-04-04 Evaluation of Suppressiveness of Soils Exhibiting Soil-Borne Disease Suppression after Long-Term Application of Organic Amendments by the Co-cultivation Method of Pathogenic Fusarium oxysporum and Indigenous Soil Microorganisms Mitsuboshi, Masahiro Kioka, Yuuzou Noguchi, Katsunori Asakawa, Susumu Microbes Environ Articles Preventive measures against soil-borne diseases need to be implemented before cultivation because very few countermeasures are available after the development of diseases. Some soils suppress soil-borne diseases despite the presence of a high population density of pathogens. If the suppressiveness of soil against soil-borne diseases may be predicted and diagnosed for crop fields, it may be possible to reduce the labor and cost associated with excessive disinfection practices. We herein evaluated the suppressiveness of soils in fields with the long-term application of organic amendments by examining the growth of pathogenic Fusarium oxysporum co-cultivated with indigenous soil microorganisms on agar plates. Soils treated with coffee residue compost or rapeseed meal showed suppressiveness against spinach wilt disease by F. oxysporum f. sp. spinaciae or spinach wilt and lettuce root rot diseases by F. oxysporum f. sp. spinaciae and F. oxysporum f. sp. lactucae, respectively, and the growth of pathogenic Fusarium spp. on agar plates was suppressed when co-cultured with microorganisms in a suspension from these soils before crop cultivation. These results indicate the potential of the growth degree of pathogenic F. oxysporum estimated by this method as a diagnostic indicator of the suppressiveness of soil associated with the inhabiting microorganisms. A correlation was found between the incidence of spinach wilt disease in spinach and the growth degree of F. oxysporum f. sp. spinaciae by this co-cultivation method, indicating that suppressiveness induced by organic amendment applications against F. oxysporum f. sp. spinaciae is evaluable by this method. The co-cultivation method may be useful for predicting and diagnosing suppressiveness against soil-borne diseases. the Japanese Society of Microbial Ecology (JSME)/the Japanese Society of Soil Microbiology (JSSM)/the Taiwan Society of Microbial Ecology (TSME)/the Japanese Society of Plant Microbe Interactions (JSPMI) 2018-03 2018-03-29 /pmc/articles/PMC5877344/ /pubmed/29459498 http://dx.doi.org/10.1264/jsme2.ME17072 Text en Copyright © 2018 by Japanese Society of Microbial Ecology / Japanese Society of Soil Microbiology / Taiwan Society of Microbial Ecology / Japanese Society of Plant Microbe Interactions. http://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Articles Mitsuboshi, Masahiro Kioka, Yuuzou Noguchi, Katsunori Asakawa, Susumu Evaluation of Suppressiveness of Soils Exhibiting Soil-Borne Disease Suppression after Long-Term Application of Organic Amendments by the Co-cultivation Method of Pathogenic Fusarium oxysporum and Indigenous Soil Microorganisms |
title | Evaluation of Suppressiveness of Soils Exhibiting Soil-Borne Disease Suppression after Long-Term Application of Organic Amendments by the Co-cultivation Method of Pathogenic Fusarium oxysporum and Indigenous Soil Microorganisms |
title_full | Evaluation of Suppressiveness of Soils Exhibiting Soil-Borne Disease Suppression after Long-Term Application of Organic Amendments by the Co-cultivation Method of Pathogenic Fusarium oxysporum and Indigenous Soil Microorganisms |
title_fullStr | Evaluation of Suppressiveness of Soils Exhibiting Soil-Borne Disease Suppression after Long-Term Application of Organic Amendments by the Co-cultivation Method of Pathogenic Fusarium oxysporum and Indigenous Soil Microorganisms |
title_full_unstemmed | Evaluation of Suppressiveness of Soils Exhibiting Soil-Borne Disease Suppression after Long-Term Application of Organic Amendments by the Co-cultivation Method of Pathogenic Fusarium oxysporum and Indigenous Soil Microorganisms |
title_short | Evaluation of Suppressiveness of Soils Exhibiting Soil-Borne Disease Suppression after Long-Term Application of Organic Amendments by the Co-cultivation Method of Pathogenic Fusarium oxysporum and Indigenous Soil Microorganisms |
title_sort | evaluation of suppressiveness of soils exhibiting soil-borne disease suppression after long-term application of organic amendments by the co-cultivation method of pathogenic fusarium oxysporum and indigenous soil microorganisms |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5877344/ https://www.ncbi.nlm.nih.gov/pubmed/29459498 http://dx.doi.org/10.1264/jsme2.ME17072 |
work_keys_str_mv | AT mitsuboshimasahiro evaluationofsuppressivenessofsoilsexhibitingsoilbornediseasesuppressionafterlongtermapplicationoforganicamendmentsbythecocultivationmethodofpathogenicfusariumoxysporumandindigenoussoilmicroorganisms AT kiokayuuzou evaluationofsuppressivenessofsoilsexhibitingsoilbornediseasesuppressionafterlongtermapplicationoforganicamendmentsbythecocultivationmethodofpathogenicfusariumoxysporumandindigenoussoilmicroorganisms AT noguchikatsunori evaluationofsuppressivenessofsoilsexhibitingsoilbornediseasesuppressionafterlongtermapplicationoforganicamendmentsbythecocultivationmethodofpathogenicfusariumoxysporumandindigenoussoilmicroorganisms AT asakawasusumu evaluationofsuppressivenessofsoilsexhibitingsoilbornediseasesuppressionafterlongtermapplicationoforganicamendmentsbythecocultivationmethodofpathogenicfusariumoxysporumandindigenoussoilmicroorganisms |