Cargando…

Three-Dimensional Graphene–RGD Peptide Nanoisland Composites That Enhance the Osteogenesis of Human Adipose-Derived Mesenchymal Stem Cells

Graphene derivatives have immense potential in stem cell research. Here, we report a three-dimensional graphene/arginine-glycine-aspartic acid (RGD) peptide nanoisland composite effective in guiding the osteogenesis of human adipose-derived mesenchymal stem cells (ADSCs). Amine-modified silica nanop...

Descripción completa

Detalles Bibliográficos
Autores principales: Kang, Ee-Seul, Kim, Da-Seul, Han, Yoojoong, Son, Hyungbin, Chung, Yong-Ho, Min, Junhong, Kim, Tae-Hyung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5877530/
https://www.ncbi.nlm.nih.gov/pubmed/29495519
http://dx.doi.org/10.3390/ijms19030669
_version_ 1783310712438784000
author Kang, Ee-Seul
Kim, Da-Seul
Han, Yoojoong
Son, Hyungbin
Chung, Yong-Ho
Min, Junhong
Kim, Tae-Hyung
author_facet Kang, Ee-Seul
Kim, Da-Seul
Han, Yoojoong
Son, Hyungbin
Chung, Yong-Ho
Min, Junhong
Kim, Tae-Hyung
author_sort Kang, Ee-Seul
collection PubMed
description Graphene derivatives have immense potential in stem cell research. Here, we report a three-dimensional graphene/arginine-glycine-aspartic acid (RGD) peptide nanoisland composite effective in guiding the osteogenesis of human adipose-derived mesenchymal stem cells (ADSCs). Amine-modified silica nanoparticles (SiNPs) were uniformly coated onto an indium tin oxide electrode (ITO), followed by graphene oxide (GO) encapsulation and electrochemical deposition of gold nanoparticles. A RGD–MAP–C peptide, with a triple-branched repeating RGD sequence and a terminal cysteine, was self-assembled onto the gold nanoparticles, generating the final three-dimensional graphene–RGD peptide nanoisland composite. We generated substrates with various gold nanoparticle–RGD peptide cluster densities, and found that the platform with the maximal number of clusters was most suitable for ADSC adhesion and spreading. Remarkably, the same platform was also highly efficient at guiding ADSC osteogenesis compared with other substrates, based on gene expression (alkaline phosphatase (ALP), runt-related transcription factor 2), enzyme activity (ALP), and calcium deposition. ADSCs induced to differentiate into osteoblasts showed higher calcium accumulations after 14–21 days than when grown on typical GO-SiNP complexes, suggesting that the platform can accelerate ADSC osteoblastic differentiation. The results demonstrate that a three-dimensional graphene–RGD peptide nanoisland composite can efficiently derive osteoblasts from mesenchymal stem cells.
format Online
Article
Text
id pubmed-5877530
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-58775302018-04-09 Three-Dimensional Graphene–RGD Peptide Nanoisland Composites That Enhance the Osteogenesis of Human Adipose-Derived Mesenchymal Stem Cells Kang, Ee-Seul Kim, Da-Seul Han, Yoojoong Son, Hyungbin Chung, Yong-Ho Min, Junhong Kim, Tae-Hyung Int J Mol Sci Article Graphene derivatives have immense potential in stem cell research. Here, we report a three-dimensional graphene/arginine-glycine-aspartic acid (RGD) peptide nanoisland composite effective in guiding the osteogenesis of human adipose-derived mesenchymal stem cells (ADSCs). Amine-modified silica nanoparticles (SiNPs) were uniformly coated onto an indium tin oxide electrode (ITO), followed by graphene oxide (GO) encapsulation and electrochemical deposition of gold nanoparticles. A RGD–MAP–C peptide, with a triple-branched repeating RGD sequence and a terminal cysteine, was self-assembled onto the gold nanoparticles, generating the final three-dimensional graphene–RGD peptide nanoisland composite. We generated substrates with various gold nanoparticle–RGD peptide cluster densities, and found that the platform with the maximal number of clusters was most suitable for ADSC adhesion and spreading. Remarkably, the same platform was also highly efficient at guiding ADSC osteogenesis compared with other substrates, based on gene expression (alkaline phosphatase (ALP), runt-related transcription factor 2), enzyme activity (ALP), and calcium deposition. ADSCs induced to differentiate into osteoblasts showed higher calcium accumulations after 14–21 days than when grown on typical GO-SiNP complexes, suggesting that the platform can accelerate ADSC osteoblastic differentiation. The results demonstrate that a three-dimensional graphene–RGD peptide nanoisland composite can efficiently derive osteoblasts from mesenchymal stem cells. MDPI 2018-02-27 /pmc/articles/PMC5877530/ /pubmed/29495519 http://dx.doi.org/10.3390/ijms19030669 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kang, Ee-Seul
Kim, Da-Seul
Han, Yoojoong
Son, Hyungbin
Chung, Yong-Ho
Min, Junhong
Kim, Tae-Hyung
Three-Dimensional Graphene–RGD Peptide Nanoisland Composites That Enhance the Osteogenesis of Human Adipose-Derived Mesenchymal Stem Cells
title Three-Dimensional Graphene–RGD Peptide Nanoisland Composites That Enhance the Osteogenesis of Human Adipose-Derived Mesenchymal Stem Cells
title_full Three-Dimensional Graphene–RGD Peptide Nanoisland Composites That Enhance the Osteogenesis of Human Adipose-Derived Mesenchymal Stem Cells
title_fullStr Three-Dimensional Graphene–RGD Peptide Nanoisland Composites That Enhance the Osteogenesis of Human Adipose-Derived Mesenchymal Stem Cells
title_full_unstemmed Three-Dimensional Graphene–RGD Peptide Nanoisland Composites That Enhance the Osteogenesis of Human Adipose-Derived Mesenchymal Stem Cells
title_short Three-Dimensional Graphene–RGD Peptide Nanoisland Composites That Enhance the Osteogenesis of Human Adipose-Derived Mesenchymal Stem Cells
title_sort three-dimensional graphene–rgd peptide nanoisland composites that enhance the osteogenesis of human adipose-derived mesenchymal stem cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5877530/
https://www.ncbi.nlm.nih.gov/pubmed/29495519
http://dx.doi.org/10.3390/ijms19030669
work_keys_str_mv AT kangeeseul threedimensionalgraphenergdpeptidenanoislandcompositesthatenhancetheosteogenesisofhumanadiposederivedmesenchymalstemcells
AT kimdaseul threedimensionalgraphenergdpeptidenanoislandcompositesthatenhancetheosteogenesisofhumanadiposederivedmesenchymalstemcells
AT hanyoojoong threedimensionalgraphenergdpeptidenanoislandcompositesthatenhancetheosteogenesisofhumanadiposederivedmesenchymalstemcells
AT sonhyungbin threedimensionalgraphenergdpeptidenanoislandcompositesthatenhancetheosteogenesisofhumanadiposederivedmesenchymalstemcells
AT chungyongho threedimensionalgraphenergdpeptidenanoislandcompositesthatenhancetheosteogenesisofhumanadiposederivedmesenchymalstemcells
AT minjunhong threedimensionalgraphenergdpeptidenanoislandcompositesthatenhancetheosteogenesisofhumanadiposederivedmesenchymalstemcells
AT kimtaehyung threedimensionalgraphenergdpeptidenanoislandcompositesthatenhancetheosteogenesisofhumanadiposederivedmesenchymalstemcells