Cargando…
Functional Analysis of Maize Silk-Specific ZmbZIP25 Promoter
ZmbZIP25 (Zea mays bZIP (basic leucine zipper) transcription factor 25) is a function-unknown protein that belongs to the D group of the bZIP transcription factor family. RNA-seq data showed that the expression of ZmbZIP25 was tissue-specific in maize silks, and this specificity was confirmed by RT-...
Autores principales: | , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2018
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5877683/ https://www.ncbi.nlm.nih.gov/pubmed/29534529 http://dx.doi.org/10.3390/ijms19030822 |
_version_ | 1783310748807593984 |
---|---|
author | Li, Wanying Yu, Dan Yu, Jingjuan Zhu, Dengyun Zhao, Qian |
author_facet | Li, Wanying Yu, Dan Yu, Jingjuan Zhu, Dengyun Zhao, Qian |
author_sort | Li, Wanying |
collection | PubMed |
description | ZmbZIP25 (Zea mays bZIP (basic leucine zipper) transcription factor 25) is a function-unknown protein that belongs to the D group of the bZIP transcription factor family. RNA-seq data showed that the expression of ZmbZIP25 was tissue-specific in maize silks, and this specificity was confirmed by RT-PCR (reverse transcription-polymerase chain reaction). In situ RNA hybridization showed that ZmbZIP25 was expressed exclusively in the xylem of maize silks. A 5′ RACE (rapid amplification of cDNA ends) assay identified an adenine residue as the transcription start site of the ZmbZIP25 gene. To characterize this silk-specific promoter, we isolated and analyzed a 2450 bp (from −2083 to +367) and a 2600 bp sequence of ZmbZIP25 (from −2083 to +517, the transcription start site was denoted +1). Stable expression assays in Arabidopsis showed that the expression of the reporter gene GUS driven by the 2450 bp ZmbZIP25 5′-flanking fragment occurred exclusively in the papillae of Arabidopsis stigmas. Furthermore, transient expression assays in maize indicated that GUS and GFP expression driven by the 2450 bp ZmbZIP25 5′-flanking sequences occurred only in maize silks and not in other tissues. However, no GUS or GFP expression was driven by the 2600 bp ZmbZIP25 5′-flanking sequences in either stable or transient expression assays. A series of deletion analyses of the 2450 bp ZmbZIP25 5′-flanking sequence was performed in transgenic Arabidopsis plants, and probable elements prediction analysis revealed the possible presence of negative regulatory elements within the 161 bp region from −1117 to −957 that were responsible for the specificity of the ZmbZIP25 5′-flanking sequence. |
format | Online Article Text |
id | pubmed-5877683 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-58776832018-04-09 Functional Analysis of Maize Silk-Specific ZmbZIP25 Promoter Li, Wanying Yu, Dan Yu, Jingjuan Zhu, Dengyun Zhao, Qian Int J Mol Sci Article ZmbZIP25 (Zea mays bZIP (basic leucine zipper) transcription factor 25) is a function-unknown protein that belongs to the D group of the bZIP transcription factor family. RNA-seq data showed that the expression of ZmbZIP25 was tissue-specific in maize silks, and this specificity was confirmed by RT-PCR (reverse transcription-polymerase chain reaction). In situ RNA hybridization showed that ZmbZIP25 was expressed exclusively in the xylem of maize silks. A 5′ RACE (rapid amplification of cDNA ends) assay identified an adenine residue as the transcription start site of the ZmbZIP25 gene. To characterize this silk-specific promoter, we isolated and analyzed a 2450 bp (from −2083 to +367) and a 2600 bp sequence of ZmbZIP25 (from −2083 to +517, the transcription start site was denoted +1). Stable expression assays in Arabidopsis showed that the expression of the reporter gene GUS driven by the 2450 bp ZmbZIP25 5′-flanking fragment occurred exclusively in the papillae of Arabidopsis stigmas. Furthermore, transient expression assays in maize indicated that GUS and GFP expression driven by the 2450 bp ZmbZIP25 5′-flanking sequences occurred only in maize silks and not in other tissues. However, no GUS or GFP expression was driven by the 2600 bp ZmbZIP25 5′-flanking sequences in either stable or transient expression assays. A series of deletion analyses of the 2450 bp ZmbZIP25 5′-flanking sequence was performed in transgenic Arabidopsis plants, and probable elements prediction analysis revealed the possible presence of negative regulatory elements within the 161 bp region from −1117 to −957 that were responsible for the specificity of the ZmbZIP25 5′-flanking sequence. MDPI 2018-03-12 /pmc/articles/PMC5877683/ /pubmed/29534529 http://dx.doi.org/10.3390/ijms19030822 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Li, Wanying Yu, Dan Yu, Jingjuan Zhu, Dengyun Zhao, Qian Functional Analysis of Maize Silk-Specific ZmbZIP25 Promoter |
title | Functional Analysis of Maize Silk-Specific ZmbZIP25 Promoter |
title_full | Functional Analysis of Maize Silk-Specific ZmbZIP25 Promoter |
title_fullStr | Functional Analysis of Maize Silk-Specific ZmbZIP25 Promoter |
title_full_unstemmed | Functional Analysis of Maize Silk-Specific ZmbZIP25 Promoter |
title_short | Functional Analysis of Maize Silk-Specific ZmbZIP25 Promoter |
title_sort | functional analysis of maize silk-specific zmbzip25 promoter |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5877683/ https://www.ncbi.nlm.nih.gov/pubmed/29534529 http://dx.doi.org/10.3390/ijms19030822 |
work_keys_str_mv | AT liwanying functionalanalysisofmaizesilkspecificzmbzip25promoter AT yudan functionalanalysisofmaizesilkspecificzmbzip25promoter AT yujingjuan functionalanalysisofmaizesilkspecificzmbzip25promoter AT zhudengyun functionalanalysisofmaizesilkspecificzmbzip25promoter AT zhaoqian functionalanalysisofmaizesilkspecificzmbzip25promoter |