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Functional Analysis of Maize Silk-Specific ZmbZIP25 Promoter

ZmbZIP25 (Zea mays bZIP (basic leucine zipper) transcription factor 25) is a function-unknown protein that belongs to the D group of the bZIP transcription factor family. RNA-seq data showed that the expression of ZmbZIP25 was tissue-specific in maize silks, and this specificity was confirmed by RT-...

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Autores principales: Li, Wanying, Yu, Dan, Yu, Jingjuan, Zhu, Dengyun, Zhao, Qian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5877683/
https://www.ncbi.nlm.nih.gov/pubmed/29534529
http://dx.doi.org/10.3390/ijms19030822
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author Li, Wanying
Yu, Dan
Yu, Jingjuan
Zhu, Dengyun
Zhao, Qian
author_facet Li, Wanying
Yu, Dan
Yu, Jingjuan
Zhu, Dengyun
Zhao, Qian
author_sort Li, Wanying
collection PubMed
description ZmbZIP25 (Zea mays bZIP (basic leucine zipper) transcription factor 25) is a function-unknown protein that belongs to the D group of the bZIP transcription factor family. RNA-seq data showed that the expression of ZmbZIP25 was tissue-specific in maize silks, and this specificity was confirmed by RT-PCR (reverse transcription-polymerase chain reaction). In situ RNA hybridization showed that ZmbZIP25 was expressed exclusively in the xylem of maize silks. A 5′ RACE (rapid amplification of cDNA ends) assay identified an adenine residue as the transcription start site of the ZmbZIP25 gene. To characterize this silk-specific promoter, we isolated and analyzed a 2450 bp (from −2083 to +367) and a 2600 bp sequence of ZmbZIP25 (from −2083 to +517, the transcription start site was denoted +1). Stable expression assays in Arabidopsis showed that the expression of the reporter gene GUS driven by the 2450 bp ZmbZIP25 5′-flanking fragment occurred exclusively in the papillae of Arabidopsis stigmas. Furthermore, transient expression assays in maize indicated that GUS and GFP expression driven by the 2450 bp ZmbZIP25 5′-flanking sequences occurred only in maize silks and not in other tissues. However, no GUS or GFP expression was driven by the 2600 bp ZmbZIP25 5′-flanking sequences in either stable or transient expression assays. A series of deletion analyses of the 2450 bp ZmbZIP25 5′-flanking sequence was performed in transgenic Arabidopsis plants, and probable elements prediction analysis revealed the possible presence of negative regulatory elements within the 161 bp region from −1117 to −957 that were responsible for the specificity of the ZmbZIP25 5′-flanking sequence.
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spelling pubmed-58776832018-04-09 Functional Analysis of Maize Silk-Specific ZmbZIP25 Promoter Li, Wanying Yu, Dan Yu, Jingjuan Zhu, Dengyun Zhao, Qian Int J Mol Sci Article ZmbZIP25 (Zea mays bZIP (basic leucine zipper) transcription factor 25) is a function-unknown protein that belongs to the D group of the bZIP transcription factor family. RNA-seq data showed that the expression of ZmbZIP25 was tissue-specific in maize silks, and this specificity was confirmed by RT-PCR (reverse transcription-polymerase chain reaction). In situ RNA hybridization showed that ZmbZIP25 was expressed exclusively in the xylem of maize silks. A 5′ RACE (rapid amplification of cDNA ends) assay identified an adenine residue as the transcription start site of the ZmbZIP25 gene. To characterize this silk-specific promoter, we isolated and analyzed a 2450 bp (from −2083 to +367) and a 2600 bp sequence of ZmbZIP25 (from −2083 to +517, the transcription start site was denoted +1). Stable expression assays in Arabidopsis showed that the expression of the reporter gene GUS driven by the 2450 bp ZmbZIP25 5′-flanking fragment occurred exclusively in the papillae of Arabidopsis stigmas. Furthermore, transient expression assays in maize indicated that GUS and GFP expression driven by the 2450 bp ZmbZIP25 5′-flanking sequences occurred only in maize silks and not in other tissues. However, no GUS or GFP expression was driven by the 2600 bp ZmbZIP25 5′-flanking sequences in either stable or transient expression assays. A series of deletion analyses of the 2450 bp ZmbZIP25 5′-flanking sequence was performed in transgenic Arabidopsis plants, and probable elements prediction analysis revealed the possible presence of negative regulatory elements within the 161 bp region from −1117 to −957 that were responsible for the specificity of the ZmbZIP25 5′-flanking sequence. MDPI 2018-03-12 /pmc/articles/PMC5877683/ /pubmed/29534529 http://dx.doi.org/10.3390/ijms19030822 Text en © 2018 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Li, Wanying
Yu, Dan
Yu, Jingjuan
Zhu, Dengyun
Zhao, Qian
Functional Analysis of Maize Silk-Specific ZmbZIP25 Promoter
title Functional Analysis of Maize Silk-Specific ZmbZIP25 Promoter
title_full Functional Analysis of Maize Silk-Specific ZmbZIP25 Promoter
title_fullStr Functional Analysis of Maize Silk-Specific ZmbZIP25 Promoter
title_full_unstemmed Functional Analysis of Maize Silk-Specific ZmbZIP25 Promoter
title_short Functional Analysis of Maize Silk-Specific ZmbZIP25 Promoter
title_sort functional analysis of maize silk-specific zmbzip25 promoter
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5877683/
https://www.ncbi.nlm.nih.gov/pubmed/29534529
http://dx.doi.org/10.3390/ijms19030822
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