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Netrin-1 Confines Rhombic Lip-Derived Neurons to the CNS
During brainstem development, newborn neurons originating from the rhombic lip embark on exceptionally long migrations to generate nuclei important for audition, movement, and respiration. Along the way, this highly motile population passes several cranial nerves yet remains confined to the CNS. We...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5877811/ https://www.ncbi.nlm.nih.gov/pubmed/29444422 http://dx.doi.org/10.1016/j.celrep.2018.01.068 |
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author | Yung, Andrea R. Druckenbrod, Noah R. Cloutier, Jean-François Wu, Zhuhao Tessier-Lavigne, Marc Goodrich, Lisa V. |
author_facet | Yung, Andrea R. Druckenbrod, Noah R. Cloutier, Jean-François Wu, Zhuhao Tessier-Lavigne, Marc Goodrich, Lisa V. |
author_sort | Yung, Andrea R. |
collection | PubMed |
description | During brainstem development, newborn neurons originating from the rhombic lip embark on exceptionally long migrations to generate nuclei important for audition, movement, and respiration. Along the way, this highly motile population passes several cranial nerves yet remains confined to the CNS. We found that Ntn1 accumulates beneath the pial surface separating the CNS from the PNS, with gaps at nerve entry sites. In mice null for Ntn1 or its receptor DCC, hindbrain neurons enter cranial nerves and migrate into the periphery. CNS neurons also escape when Ntn1 is selectively lost from the sub-pial region (SPR), and conversely, expression of Ntn1 throughout the mutant hindbrain can prevent their departure. These findings identify a permissive role for Ntn1 in maintaining the CNS-PNS boundary. We propose that Ntn1 confines rhombic lip-derived neurons by providing a preferred substrate for tangentially migrating neurons in the SPR, preventing their entry into nerve roots. |
format | Online Article Text |
id | pubmed-5877811 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
record_format | MEDLINE/PubMed |
spelling | pubmed-58778112018-03-30 Netrin-1 Confines Rhombic Lip-Derived Neurons to the CNS Yung, Andrea R. Druckenbrod, Noah R. Cloutier, Jean-François Wu, Zhuhao Tessier-Lavigne, Marc Goodrich, Lisa V. Cell Rep Article During brainstem development, newborn neurons originating from the rhombic lip embark on exceptionally long migrations to generate nuclei important for audition, movement, and respiration. Along the way, this highly motile population passes several cranial nerves yet remains confined to the CNS. We found that Ntn1 accumulates beneath the pial surface separating the CNS from the PNS, with gaps at nerve entry sites. In mice null for Ntn1 or its receptor DCC, hindbrain neurons enter cranial nerves and migrate into the periphery. CNS neurons also escape when Ntn1 is selectively lost from the sub-pial region (SPR), and conversely, expression of Ntn1 throughout the mutant hindbrain can prevent their departure. These findings identify a permissive role for Ntn1 in maintaining the CNS-PNS boundary. We propose that Ntn1 confines rhombic lip-derived neurons by providing a preferred substrate for tangentially migrating neurons in the SPR, preventing their entry into nerve roots. 2018-02-13 /pmc/articles/PMC5877811/ /pubmed/29444422 http://dx.doi.org/10.1016/j.celrep.2018.01.068 Text en This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Yung, Andrea R. Druckenbrod, Noah R. Cloutier, Jean-François Wu, Zhuhao Tessier-Lavigne, Marc Goodrich, Lisa V. Netrin-1 Confines Rhombic Lip-Derived Neurons to the CNS |
title | Netrin-1 Confines Rhombic Lip-Derived Neurons to the CNS |
title_full | Netrin-1 Confines Rhombic Lip-Derived Neurons to the CNS |
title_fullStr | Netrin-1 Confines Rhombic Lip-Derived Neurons to the CNS |
title_full_unstemmed | Netrin-1 Confines Rhombic Lip-Derived Neurons to the CNS |
title_short | Netrin-1 Confines Rhombic Lip-Derived Neurons to the CNS |
title_sort | netrin-1 confines rhombic lip-derived neurons to the cns |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5877811/ https://www.ncbi.nlm.nih.gov/pubmed/29444422 http://dx.doi.org/10.1016/j.celrep.2018.01.068 |
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