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A sensitive and innovative detection method for rapid C-reactive proteins analysis based on a micro-fluxgate sensor system

A sensitive and innovative assay system based on a micro-MEMS-fluxgate sensor and immunomagnetic beads-labels was developed for the rapid analysis of C-reactive proteins (CRP). The fluxgate sensor presented in this study was fabricated through standard micro-electro-mechanical system technology. A m...

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Autores principales: Guo, Lei, Yang, Zhen, Zhi, Shaotao, Feng, Zhu, Lei, Chong, Zhou, Yong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5877836/
https://www.ncbi.nlm.nih.gov/pubmed/29601593
http://dx.doi.org/10.1371/journal.pone.0194631
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author Guo, Lei
Yang, Zhen
Zhi, Shaotao
Feng, Zhu
Lei, Chong
Zhou, Yong
author_facet Guo, Lei
Yang, Zhen
Zhi, Shaotao
Feng, Zhu
Lei, Chong
Zhou, Yong
author_sort Guo, Lei
collection PubMed
description A sensitive and innovative assay system based on a micro-MEMS-fluxgate sensor and immunomagnetic beads-labels was developed for the rapid analysis of C-reactive proteins (CRP). The fluxgate sensor presented in this study was fabricated through standard micro-electro-mechanical system technology. A multi-loop magnetic core made of Fe-based amorphous ribbon was employed as the sensing element, and 3-D solenoid copper coils were used to control the sensing core. Antibody-conjugated immunomagnetic microbeads were strategically utilized as signal tags to label the CRP via the specific conjugation of CRP to polyclonal CRP antibodies. Separate Au film substrates were applied as immunoplatforms to immobilize CRP-beads labels through classical sandwich assays. Detection and quantification of the CRP at different concentrations were implemented by detecting the stray field of CRP labeled magnetic beads using the newly-developed micro-fluxgate sensor. The resulting system exhibited the required sensitivity, stability, reproducibility, and selectivity. A detection limit as low as 0.002 μg/mL CRP with a linearity range from 0.002 μg/mL to 10 μg/mL was achieved, and this suggested that the proposed biosystem possesses high sensitivity. In addition to the extremely low detection limit, the proposed method can be easily manipulated and possesses a quick response time. The response time of our sensor was less than 5 s, and the entire detection period for CRP analysis can be completed in less than 30 min using the current method. Given the detection performance and other advantages such as miniaturization, excellent stability and specificity, the proposed biosensor can be considered as a potential candidate for the rapid analysis of CRP, especially for point-of-care platforms.
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spelling pubmed-58778362018-04-13 A sensitive and innovative detection method for rapid C-reactive proteins analysis based on a micro-fluxgate sensor system Guo, Lei Yang, Zhen Zhi, Shaotao Feng, Zhu Lei, Chong Zhou, Yong PLoS One Research Article A sensitive and innovative assay system based on a micro-MEMS-fluxgate sensor and immunomagnetic beads-labels was developed for the rapid analysis of C-reactive proteins (CRP). The fluxgate sensor presented in this study was fabricated through standard micro-electro-mechanical system technology. A multi-loop magnetic core made of Fe-based amorphous ribbon was employed as the sensing element, and 3-D solenoid copper coils were used to control the sensing core. Antibody-conjugated immunomagnetic microbeads were strategically utilized as signal tags to label the CRP via the specific conjugation of CRP to polyclonal CRP antibodies. Separate Au film substrates were applied as immunoplatforms to immobilize CRP-beads labels through classical sandwich assays. Detection and quantification of the CRP at different concentrations were implemented by detecting the stray field of CRP labeled magnetic beads using the newly-developed micro-fluxgate sensor. The resulting system exhibited the required sensitivity, stability, reproducibility, and selectivity. A detection limit as low as 0.002 μg/mL CRP with a linearity range from 0.002 μg/mL to 10 μg/mL was achieved, and this suggested that the proposed biosystem possesses high sensitivity. In addition to the extremely low detection limit, the proposed method can be easily manipulated and possesses a quick response time. The response time of our sensor was less than 5 s, and the entire detection period for CRP analysis can be completed in less than 30 min using the current method. Given the detection performance and other advantages such as miniaturization, excellent stability and specificity, the proposed biosensor can be considered as a potential candidate for the rapid analysis of CRP, especially for point-of-care platforms. Public Library of Science 2018-03-30 /pmc/articles/PMC5877836/ /pubmed/29601593 http://dx.doi.org/10.1371/journal.pone.0194631 Text en © 2018 Guo et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Guo, Lei
Yang, Zhen
Zhi, Shaotao
Feng, Zhu
Lei, Chong
Zhou, Yong
A sensitive and innovative detection method for rapid C-reactive proteins analysis based on a micro-fluxgate sensor system
title A sensitive and innovative detection method for rapid C-reactive proteins analysis based on a micro-fluxgate sensor system
title_full A sensitive and innovative detection method for rapid C-reactive proteins analysis based on a micro-fluxgate sensor system
title_fullStr A sensitive and innovative detection method for rapid C-reactive proteins analysis based on a micro-fluxgate sensor system
title_full_unstemmed A sensitive and innovative detection method for rapid C-reactive proteins analysis based on a micro-fluxgate sensor system
title_short A sensitive and innovative detection method for rapid C-reactive proteins analysis based on a micro-fluxgate sensor system
title_sort sensitive and innovative detection method for rapid c-reactive proteins analysis based on a micro-fluxgate sensor system
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5877836/
https://www.ncbi.nlm.nih.gov/pubmed/29601593
http://dx.doi.org/10.1371/journal.pone.0194631
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