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Impact of F1Fo-ATP-synthase dimer assembly factors on mitochondrial function and organismic aging

In aerobic organisms, mitochondrial F(1)F(o)-ATP-synthase is the major site of ATP production. Beside this fundamental role, the protein complex is involved in shaping and maintenance of cristae. Previous electron microscopic studies identified the dissociation of F(1)F(o)-ATP-synthase dimers and ol...

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Autores principales: Rampello, Nadia G, Stenger, Maria, Westermann, Benedikt, Osiewacz, Heinz D
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Shared Science Publishers OG 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5878687/
https://www.ncbi.nlm.nih.gov/pubmed/29610761
http://dx.doi.org/10.15698/mic2018.04.625
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author Rampello, Nadia G
Stenger, Maria
Westermann, Benedikt
Osiewacz, Heinz D
author_facet Rampello, Nadia G
Stenger, Maria
Westermann, Benedikt
Osiewacz, Heinz D
author_sort Rampello, Nadia G
collection PubMed
description In aerobic organisms, mitochondrial F(1)F(o)-ATP-synthase is the major site of ATP production. Beside this fundamental role, the protein complex is involved in shaping and maintenance of cristae. Previous electron microscopic studies identified the dissociation of F(1)F(o)-ATP-synthase dimers and oligomers during organismic aging correlating with a massive remodeling of the mitochondrial inner membrane. Here we report results aimed to experimentally proof this impact and to obtain further insights into the control of these processes. We focused on the role of the two dimer assembly factors PaATPE and PaATPG of the aging model Podospora anserina. Ablation of either protein strongly affects mitochondrial function and leads to an accumulation of senescence markers demonstrating that the inhibition of dimer formation negatively influences vital functions and accelerates organismic aging. Our data validate a model that links mitochondrial membrane remodeling to aging and identify specific molecular components triggering this process.
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spelling pubmed-58786872018-04-02 Impact of F1Fo-ATP-synthase dimer assembly factors on mitochondrial function and organismic aging Rampello, Nadia G Stenger, Maria Westermann, Benedikt Osiewacz, Heinz D Microb Cell Microbiology In aerobic organisms, mitochondrial F(1)F(o)-ATP-synthase is the major site of ATP production. Beside this fundamental role, the protein complex is involved in shaping and maintenance of cristae. Previous electron microscopic studies identified the dissociation of F(1)F(o)-ATP-synthase dimers and oligomers during organismic aging correlating with a massive remodeling of the mitochondrial inner membrane. Here we report results aimed to experimentally proof this impact and to obtain further insights into the control of these processes. We focused on the role of the two dimer assembly factors PaATPE and PaATPG of the aging model Podospora anserina. Ablation of either protein strongly affects mitochondrial function and leads to an accumulation of senescence markers demonstrating that the inhibition of dimer formation negatively influences vital functions and accelerates organismic aging. Our data validate a model that links mitochondrial membrane remodeling to aging and identify specific molecular components triggering this process. Shared Science Publishers OG 2018-01-30 /pmc/articles/PMC5878687/ /pubmed/29610761 http://dx.doi.org/10.15698/mic2018.04.625 Text en https://creativecommons.org/licenses/by/4.0/ This is an open-access article released under the terms of the Creative Commons Attribution (CC BY) license, which allows the unrestricted use, distribution, and reproduction in any medium, provided the original author and source are acknowledged.
spellingShingle Microbiology
Rampello, Nadia G
Stenger, Maria
Westermann, Benedikt
Osiewacz, Heinz D
Impact of F1Fo-ATP-synthase dimer assembly factors on mitochondrial function and organismic aging
title Impact of F1Fo-ATP-synthase dimer assembly factors on mitochondrial function and organismic aging
title_full Impact of F1Fo-ATP-synthase dimer assembly factors on mitochondrial function and organismic aging
title_fullStr Impact of F1Fo-ATP-synthase dimer assembly factors on mitochondrial function and organismic aging
title_full_unstemmed Impact of F1Fo-ATP-synthase dimer assembly factors on mitochondrial function and organismic aging
title_short Impact of F1Fo-ATP-synthase dimer assembly factors on mitochondrial function and organismic aging
title_sort impact of f1fo-atp-synthase dimer assembly factors on mitochondrial function and organismic aging
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5878687/
https://www.ncbi.nlm.nih.gov/pubmed/29610761
http://dx.doi.org/10.15698/mic2018.04.625
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