Identification of Minimal p53 Promoter Region Regulated by MALAT1 in Human Lung Adenocarcinoma Cells

The MALAT1 long noncoding RNA is strongly linked to cancer progression. Here we report a MALAT1 function in repressing the promoter of p53 (TP53) tumor suppressor gene. p21 and FAS, well-known p53 targets, were upregulated by MALAT1 knockdown in A549 human lung adenocarcinoma cells. We found that th...

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Autores principales: Tano, Keiko, Onoguchi-Mizutani, Rena, Yeasmin, Fouzia, Uchiumi, Fumiaki, Suzuki, Yutaka, Yada, Tetsushi, Akimitsu, Nobuyoshi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Genetics
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5879451/
https://www.ncbi.nlm.nih.gov/pubmed/29632545
http://dx.doi.org/10.3389/fgene.2017.00208
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author Tano, Keiko
Onoguchi-Mizutani, Rena
Yeasmin, Fouzia
Uchiumi, Fumiaki
Suzuki, Yutaka
Yada, Tetsushi
Akimitsu, Nobuyoshi
author_facet Tano, Keiko
Onoguchi-Mizutani, Rena
Yeasmin, Fouzia
Uchiumi, Fumiaki
Suzuki, Yutaka
Yada, Tetsushi
Akimitsu, Nobuyoshi
author_sort Tano, Keiko
collection PubMed
description The MALAT1 long noncoding RNA is strongly linked to cancer progression. Here we report a MALAT1 function in repressing the promoter of p53 (TP53) tumor suppressor gene. p21 and FAS, well-known p53 targets, were upregulated by MALAT1 knockdown in A549 human lung adenocarcinoma cells. We found that these upregulations were mediated by transcriptional activation of p53 through MALAT1 depletion. In addition, we identified a minimal MALAT1-responsive region in the P1 promoter of p53 gene. Flow cytometry analysis revealed that MALAT1-depleted cells exhibited G1 cell cycle arrest. These results suggest that MALAT1 affects the expression of p53 target genes through repressing p53 promoter activity, leading to influence the cell cycle progression.
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spelling pubmed-58794512018-04-09 Identification of Minimal p53 Promoter Region Regulated by MALAT1 in Human Lung Adenocarcinoma Cells Tano, Keiko Onoguchi-Mizutani, Rena Yeasmin, Fouzia Uchiumi, Fumiaki Suzuki, Yutaka Yada, Tetsushi Akimitsu, Nobuyoshi Front Genet Genetics The MALAT1 long noncoding RNA is strongly linked to cancer progression. Here we report a MALAT1 function in repressing the promoter of p53 (TP53) tumor suppressor gene. p21 and FAS, well-known p53 targets, were upregulated by MALAT1 knockdown in A549 human lung adenocarcinoma cells. We found that these upregulations were mediated by transcriptional activation of p53 through MALAT1 depletion. In addition, we identified a minimal MALAT1-responsive region in the P1 promoter of p53 gene. Flow cytometry analysis revealed that MALAT1-depleted cells exhibited G1 cell cycle arrest. These results suggest that MALAT1 affects the expression of p53 target genes through repressing p53 promoter activity, leading to influence the cell cycle progression. Frontiers Media S.A. 2018-03-26 /pmc/articles/PMC5879451/ /pubmed/29632545 http://dx.doi.org/10.3389/fgene.2017.00208 Text en Copyright © 2018 Tano, Onoguchi-Mizutani, Yeasmin, Uchiumi, Suzuki, Yada and Akimitsu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Genetics
Tano, Keiko
Onoguchi-Mizutani, Rena
Yeasmin, Fouzia
Uchiumi, Fumiaki
Suzuki, Yutaka
Yada, Tetsushi
Akimitsu, Nobuyoshi
Identification of Minimal p53 Promoter Region Regulated by MALAT1 in Human Lung Adenocarcinoma Cells
title Identification of Minimal p53 Promoter Region Regulated by MALAT1 in Human Lung Adenocarcinoma Cells
title_full Identification of Minimal p53 Promoter Region Regulated by MALAT1 in Human Lung Adenocarcinoma Cells
title_fullStr Identification of Minimal p53 Promoter Region Regulated by MALAT1 in Human Lung Adenocarcinoma Cells
title_full_unstemmed Identification of Minimal p53 Promoter Region Regulated by MALAT1 in Human Lung Adenocarcinoma Cells
title_short Identification of Minimal p53 Promoter Region Regulated by MALAT1 in Human Lung Adenocarcinoma Cells
title_sort identification of minimal p53 promoter region regulated by malat1 in human lung adenocarcinoma cells
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5879451/
https://www.ncbi.nlm.nih.gov/pubmed/29632545
http://dx.doi.org/10.3389/fgene.2017.00208
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