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5′ Rapid Amplification of cDNA Ends and Illumina MiSeq Reveals B Cell Receptor Features in Healthy Adults, Adults With Chronic HIV-1 Infection, Cord Blood, and Humanized Mice

Using 5′ rapid amplification of cDNA ends, Illumina MiSeq, and basic flow cytometry, we systematically analyzed the expressed B cell receptor (BCR) repertoire in 14 healthy adult PBMCs, 5 HIV-1+ adult PBMCs, 5 cord blood samples, and 3 HIS-CD4/B mice, examining the full-length variable region of μ,...

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Detalles Bibliográficos
Autores principales:	Waltari, Eric, Jia, Manxue, Jiang, Caroline S., Lu, Hong, Huang, Jing, Fernandez, Cristina, Finzi, Andrés, Kaufmann, Daniel E., Markowitz, Martin, Tsuji, Moriya, Wu, Xueling
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2018
Materias:	Immunology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5879793/ https://www.ncbi.nlm.nih.gov/pubmed/29632541 http://dx.doi.org/10.3389/fimmu.2018.00628

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