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Comparative genome analysis of 24 bovine-associated Staphylococcus isolates with special focus on the putative virulence genes

Non-aureus staphylococci (NAS) are most commonly isolated from subclinical mastitis. Different NAS species may, however, have diverse effects on the inflammatory response in the udder. We determined the genome sequences of 20 staphylococcal isolates from clinical or subclinical bovine mastitis, belo...

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Autores principales: Åvall-Jääskeläinen, Silja, Taponen, Suvi, Kant, Ravi, Paulin, Lars, Blom, Jochen, Palva, Airi, Koort, Joanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: PeerJ Inc. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5880176/
https://www.ncbi.nlm.nih.gov/pubmed/29610707
http://dx.doi.org/10.7717/peerj.4560
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author Åvall-Jääskeläinen, Silja
Taponen, Suvi
Kant, Ravi
Paulin, Lars
Blom, Jochen
Palva, Airi
Koort, Joanna
author_facet Åvall-Jääskeläinen, Silja
Taponen, Suvi
Kant, Ravi
Paulin, Lars
Blom, Jochen
Palva, Airi
Koort, Joanna
author_sort Åvall-Jääskeläinen, Silja
collection PubMed
description Non-aureus staphylococci (NAS) are most commonly isolated from subclinical mastitis. Different NAS species may, however, have diverse effects on the inflammatory response in the udder. We determined the genome sequences of 20 staphylococcal isolates from clinical or subclinical bovine mastitis, belonging to the NAS species Staphylococcus agnetis, S. chromogenes, and S. simulans, and focused on the putative virulence factor genes present in the genomes. For comparison we used our previously published genome sequences of four S. aureus isolates from bovine mastitis. The pan-genome and core genomes of the non-aureus isolates were characterized. After that, putative virulence factor orthologues were searched in silico. We compared the presence of putative virulence factors in the NAS species and S. aureus and evaluated the potential association between bacterial genotype and type of mastitis (clinical vs. subclinical). The NAS isolates had much less virulence gene orthologues than the S. aureus isolates. One third of the virulence genes were detected only in S. aureus. About 100 virulence genes were present in all S. aureus isolates, compared to about 40 to 50 in each NAS isolate. S. simulans differed the most. Several of the virulence genes detected among NAS were harbored only by S. simulans, but it also lacked a number of genes present both in S. agnetis and S. chromogenes. The type of mastitis was not associated with any specific virulence gene profile. It seems that the virulence gene profiles or cumulative number of different virulence genes are not directly associated with the type of mastitis (clinical or subclinical), indicating that host derived factors such as the immune status play a pivotal role in the manifestation of mastitis.
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spelling pubmed-58801762018-04-02 Comparative genome analysis of 24 bovine-associated Staphylococcus isolates with special focus on the putative virulence genes Åvall-Jääskeläinen, Silja Taponen, Suvi Kant, Ravi Paulin, Lars Blom, Jochen Palva, Airi Koort, Joanna PeerJ Genetics Non-aureus staphylococci (NAS) are most commonly isolated from subclinical mastitis. Different NAS species may, however, have diverse effects on the inflammatory response in the udder. We determined the genome sequences of 20 staphylococcal isolates from clinical or subclinical bovine mastitis, belonging to the NAS species Staphylococcus agnetis, S. chromogenes, and S. simulans, and focused on the putative virulence factor genes present in the genomes. For comparison we used our previously published genome sequences of four S. aureus isolates from bovine mastitis. The pan-genome and core genomes of the non-aureus isolates were characterized. After that, putative virulence factor orthologues were searched in silico. We compared the presence of putative virulence factors in the NAS species and S. aureus and evaluated the potential association between bacterial genotype and type of mastitis (clinical vs. subclinical). The NAS isolates had much less virulence gene orthologues than the S. aureus isolates. One third of the virulence genes were detected only in S. aureus. About 100 virulence genes were present in all S. aureus isolates, compared to about 40 to 50 in each NAS isolate. S. simulans differed the most. Several of the virulence genes detected among NAS were harbored only by S. simulans, but it also lacked a number of genes present both in S. agnetis and S. chromogenes. The type of mastitis was not associated with any specific virulence gene profile. It seems that the virulence gene profiles or cumulative number of different virulence genes are not directly associated with the type of mastitis (clinical or subclinical), indicating that host derived factors such as the immune status play a pivotal role in the manifestation of mastitis. PeerJ Inc. 2018-03-30 /pmc/articles/PMC5880176/ /pubmed/29610707 http://dx.doi.org/10.7717/peerj.4560 Text en ©2018 Åvall-Jääskeläinen et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited.
spellingShingle Genetics
Åvall-Jääskeläinen, Silja
Taponen, Suvi
Kant, Ravi
Paulin, Lars
Blom, Jochen
Palva, Airi
Koort, Joanna
Comparative genome analysis of 24 bovine-associated Staphylococcus isolates with special focus on the putative virulence genes
title Comparative genome analysis of 24 bovine-associated Staphylococcus isolates with special focus on the putative virulence genes
title_full Comparative genome analysis of 24 bovine-associated Staphylococcus isolates with special focus on the putative virulence genes
title_fullStr Comparative genome analysis of 24 bovine-associated Staphylococcus isolates with special focus on the putative virulence genes
title_full_unstemmed Comparative genome analysis of 24 bovine-associated Staphylococcus isolates with special focus on the putative virulence genes
title_short Comparative genome analysis of 24 bovine-associated Staphylococcus isolates with special focus on the putative virulence genes
title_sort comparative genome analysis of 24 bovine-associated staphylococcus isolates with special focus on the putative virulence genes
topic Genetics
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5880176/
https://www.ncbi.nlm.nih.gov/pubmed/29610707
http://dx.doi.org/10.7717/peerj.4560
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