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Proteomics and transcriptomics analyses of ataxia telangiectasia cells treated with Dexamethasone
Ataxia telangiectasia (A-T) is an incurable and rare hereditary syndrome. In recent times, treatment with glucocorticoid analogues has been shown to improve the neurological symptoms that characterize this condition, but the molecular mechanism of action of these analogues remains unknown. Hence, th...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5880408/ https://www.ncbi.nlm.nih.gov/pubmed/29608596 http://dx.doi.org/10.1371/journal.pone.0195388 |
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author | Menotta, Michele Orazi, Sara Gioacchini, Anna Maria Spapperi, Chiara Ricci, Anastasia Chessa, Luciana Magnani, Mauro |
author_facet | Menotta, Michele Orazi, Sara Gioacchini, Anna Maria Spapperi, Chiara Ricci, Anastasia Chessa, Luciana Magnani, Mauro |
author_sort | Menotta, Michele |
collection | PubMed |
description | Ataxia telangiectasia (A-T) is an incurable and rare hereditary syndrome. In recent times, treatment with glucocorticoid analogues has been shown to improve the neurological symptoms that characterize this condition, but the molecular mechanism of action of these analogues remains unknown. Hence, the aim of this study was to gain insight into the molecular mechanism of action of glucocorticoid analogues in the treatment of A-T by investigating the role of Dexamethasone (Dexa) in A-T lymphoblastoid cell lines. We used 2DE and tandem MS to identify proteins that were influenced by the drug in A-T cells but not in healthy cells. Thirty-four proteins were defined out of a total of 746±63. Transcriptome analysis was performed by microarray and showed the differential expression of 599 A-T and 362 wild type (WT) genes and a healthy un-matching between protein abundance and the corresponding gene expression variation. The proteomic and transcriptomic profiles allowed the network pathway analysis to pinpoint the biological and molecular functions affected by Dexamethasone in Dexa-treated cells. The present integrated study provides evidence of the molecular mechanism of action of Dexamethasone in an A-T cellular model but also the broader effects of the drug in other tested cell lines. |
format | Online Article Text |
id | pubmed-5880408 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-58804082018-04-13 Proteomics and transcriptomics analyses of ataxia telangiectasia cells treated with Dexamethasone Menotta, Michele Orazi, Sara Gioacchini, Anna Maria Spapperi, Chiara Ricci, Anastasia Chessa, Luciana Magnani, Mauro PLoS One Research Article Ataxia telangiectasia (A-T) is an incurable and rare hereditary syndrome. In recent times, treatment with glucocorticoid analogues has been shown to improve the neurological symptoms that characterize this condition, but the molecular mechanism of action of these analogues remains unknown. Hence, the aim of this study was to gain insight into the molecular mechanism of action of glucocorticoid analogues in the treatment of A-T by investigating the role of Dexamethasone (Dexa) in A-T lymphoblastoid cell lines. We used 2DE and tandem MS to identify proteins that were influenced by the drug in A-T cells but not in healthy cells. Thirty-four proteins were defined out of a total of 746±63. Transcriptome analysis was performed by microarray and showed the differential expression of 599 A-T and 362 wild type (WT) genes and a healthy un-matching between protein abundance and the corresponding gene expression variation. The proteomic and transcriptomic profiles allowed the network pathway analysis to pinpoint the biological and molecular functions affected by Dexamethasone in Dexa-treated cells. The present integrated study provides evidence of the molecular mechanism of action of Dexamethasone in an A-T cellular model but also the broader effects of the drug in other tested cell lines. Public Library of Science 2018-04-02 /pmc/articles/PMC5880408/ /pubmed/29608596 http://dx.doi.org/10.1371/journal.pone.0195388 Text en © 2018 Menotta et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Menotta, Michele Orazi, Sara Gioacchini, Anna Maria Spapperi, Chiara Ricci, Anastasia Chessa, Luciana Magnani, Mauro Proteomics and transcriptomics analyses of ataxia telangiectasia cells treated with Dexamethasone |
title | Proteomics and transcriptomics analyses of ataxia telangiectasia cells treated with Dexamethasone |
title_full | Proteomics and transcriptomics analyses of ataxia telangiectasia cells treated with Dexamethasone |
title_fullStr | Proteomics and transcriptomics analyses of ataxia telangiectasia cells treated with Dexamethasone |
title_full_unstemmed | Proteomics and transcriptomics analyses of ataxia telangiectasia cells treated with Dexamethasone |
title_short | Proteomics and transcriptomics analyses of ataxia telangiectasia cells treated with Dexamethasone |
title_sort | proteomics and transcriptomics analyses of ataxia telangiectasia cells treated with dexamethasone |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5880408/ https://www.ncbi.nlm.nih.gov/pubmed/29608596 http://dx.doi.org/10.1371/journal.pone.0195388 |
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