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Prevalence and characteristics of extended-spectrum β-lactamase-producing Escherichia coli in domestic and imported chicken meats in Japan

The purpose of this study was to investigate the prevalence of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (ESBL-Ec) in retail chicken meats in Japan. Fifty-six domestic and 50 imported (Brazil, n=36; United States, n=8; Thailand, n=6) chicken meat samples were analyzed. The 162...

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Autores principales: NAHAR, Azimun, AWASTHI, Sharda Prasad, HATANAKA, Noritoshi, OKUNO, Kentaro, HOANG, Phuong Hoai, HASSAN, Jayedul, HINENOYA, Atsushi, YAMASAKI, Shinji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5880835/
https://www.ncbi.nlm.nih.gov/pubmed/29434117
http://dx.doi.org/10.1292/jvms.17-0708
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author NAHAR, Azimun
AWASTHI, Sharda Prasad
HATANAKA, Noritoshi
OKUNO, Kentaro
HOANG, Phuong Hoai
HASSAN, Jayedul
HINENOYA, Atsushi
YAMASAKI, Shinji
author_facet NAHAR, Azimun
AWASTHI, Sharda Prasad
HATANAKA, Noritoshi
OKUNO, Kentaro
HOANG, Phuong Hoai
HASSAN, Jayedul
HINENOYA, Atsushi
YAMASAKI, Shinji
author_sort NAHAR, Azimun
collection PubMed
description The purpose of this study was to investigate the prevalence of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (ESBL-Ec) in retail chicken meats in Japan. Fifty-six domestic and 50 imported (Brazil, n=36; United States, n=8; Thailand, n=6) chicken meat samples were analyzed. The 162 ESBL-Ec included 111 from 43 (77%) domestic samples and 51 from 26 (52%) Brazilian samples. Fifty-three and 30 of 111 and 51 ESBL-Ec from domestic and Brazilian chickens, respectively, were selected for ESBL genotyping. The bla(CTX-M) (91%), bla(TEM) (36%) and bla(SHV) (15%) genes were detected in ESBL-Ec isolated from domestic chickens, whereas bla(CTX-M) (100%) and bla(TEM) (20%) were detected in ESBL-Ec isolated from imported chickens. Among the bla(CTX-M) group, bla(CTX-M-2) (45%) and bla(CTX-M-1) (34%) were prevalent in domestic chicken isolates, whereas bla(CTX-M-2) (53%) and bla(CTX-M-8) (43%) were prevalent in imported chicken isolates. Domestic chicken isolates were mostly resistant to tetracycline (83%), followed by streptomycin (70%) and nalidixic acid (62%). Imported chicken isolates were resistant to streptomycin (77%), followed by nalidixic acid (63%) and tetracycline (57%). Notably, extensive multidrug resistance was detected in 60% (32/53) and 70% (21/30) ESBL-Ec from domestic and imported chickens, respectively. Virulence genes associated with diarrheagenic and extra-intestinal pathogenic E. coli were detected in ESBL-Ec isolated from domestic and imported chickens. These data suggest that ESBL-Ec in retail chicken meats could be a potential reservoir for antimicrobial resistance determinants and that some are potentially harmful to humans.
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spelling pubmed-58808352018-04-05 Prevalence and characteristics of extended-spectrum β-lactamase-producing Escherichia coli in domestic and imported chicken meats in Japan NAHAR, Azimun AWASTHI, Sharda Prasad HATANAKA, Noritoshi OKUNO, Kentaro HOANG, Phuong Hoai HASSAN, Jayedul HINENOYA, Atsushi YAMASAKI, Shinji J Vet Med Sci Public Health The purpose of this study was to investigate the prevalence of extended-spectrum β-lactamase (ESBL)-producing Escherichia coli (ESBL-Ec) in retail chicken meats in Japan. Fifty-six domestic and 50 imported (Brazil, n=36; United States, n=8; Thailand, n=6) chicken meat samples were analyzed. The 162 ESBL-Ec included 111 from 43 (77%) domestic samples and 51 from 26 (52%) Brazilian samples. Fifty-three and 30 of 111 and 51 ESBL-Ec from domestic and Brazilian chickens, respectively, were selected for ESBL genotyping. The bla(CTX-M) (91%), bla(TEM) (36%) and bla(SHV) (15%) genes were detected in ESBL-Ec isolated from domestic chickens, whereas bla(CTX-M) (100%) and bla(TEM) (20%) were detected in ESBL-Ec isolated from imported chickens. Among the bla(CTX-M) group, bla(CTX-M-2) (45%) and bla(CTX-M-1) (34%) were prevalent in domestic chicken isolates, whereas bla(CTX-M-2) (53%) and bla(CTX-M-8) (43%) were prevalent in imported chicken isolates. Domestic chicken isolates were mostly resistant to tetracycline (83%), followed by streptomycin (70%) and nalidixic acid (62%). Imported chicken isolates were resistant to streptomycin (77%), followed by nalidixic acid (63%) and tetracycline (57%). Notably, extensive multidrug resistance was detected in 60% (32/53) and 70% (21/30) ESBL-Ec from domestic and imported chickens, respectively. Virulence genes associated with diarrheagenic and extra-intestinal pathogenic E. coli were detected in ESBL-Ec isolated from domestic and imported chickens. These data suggest that ESBL-Ec in retail chicken meats could be a potential reservoir for antimicrobial resistance determinants and that some are potentially harmful to humans. The Japanese Society of Veterinary Science 2018-02-12 2018-03 /pmc/articles/PMC5880835/ /pubmed/29434117 http://dx.doi.org/10.1292/jvms.17-0708 Text en ©2018 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Public Health
NAHAR, Azimun
AWASTHI, Sharda Prasad
HATANAKA, Noritoshi
OKUNO, Kentaro
HOANG, Phuong Hoai
HASSAN, Jayedul
HINENOYA, Atsushi
YAMASAKI, Shinji
Prevalence and characteristics of extended-spectrum β-lactamase-producing Escherichia coli in domestic and imported chicken meats in Japan
title Prevalence and characteristics of extended-spectrum β-lactamase-producing Escherichia coli in domestic and imported chicken meats in Japan
title_full Prevalence and characteristics of extended-spectrum β-lactamase-producing Escherichia coli in domestic and imported chicken meats in Japan
title_fullStr Prevalence and characteristics of extended-spectrum β-lactamase-producing Escherichia coli in domestic and imported chicken meats in Japan
title_full_unstemmed Prevalence and characteristics of extended-spectrum β-lactamase-producing Escherichia coli in domestic and imported chicken meats in Japan
title_short Prevalence and characteristics of extended-spectrum β-lactamase-producing Escherichia coli in domestic and imported chicken meats in Japan
title_sort prevalence and characteristics of extended-spectrum β-lactamase-producing escherichia coli in domestic and imported chicken meats in japan
topic Public Health
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5880835/
https://www.ncbi.nlm.nih.gov/pubmed/29434117
http://dx.doi.org/10.1292/jvms.17-0708
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