Efficacy of mechanical micro-vibration in the development of bovine embryos during in vitro maturation and culture

It is currently unclear how mechanical micro-vibration affects the in vitro culture of embryos in Japanese Black cow. In the experimental groups, immature oocytes and fertilized embryos were cultured using the micro-vibration culture system with the vibration set for 5 sec at intervals of 60 min and...

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Autores principales: TAKAHASHI, Masahiro, HONDA, Tatsutoshi, HATOYA, Shingo, INABA, Toshio, KAWATE, Noritoshi, TAMADA, Hiromichi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2018
Materias:
Theriogenology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5880838/
https://www.ncbi.nlm.nih.gov/pubmed/29415921
http://dx.doi.org/10.1292/jvms.17-0607
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author TAKAHASHI, Masahiro
HONDA, Tatsutoshi
HATOYA, Shingo
INABA, Toshio
KAWATE, Noritoshi
TAMADA, Hiromichi
author_facet TAKAHASHI, Masahiro
HONDA, Tatsutoshi
HATOYA, Shingo
INABA, Toshio
KAWATE, Noritoshi
TAMADA, Hiromichi
author_sort TAKAHASHI, Masahiro
collection PubMed
description It is currently unclear how mechanical micro-vibration affects the in vitro culture of embryos in Japanese Black cow. In the experimental groups, immature oocytes and fertilized embryos were cultured using the micro-vibration culture system with the vibration set for 5 sec at intervals of 60 min and frequency of 20, 40 or 80 Hz, respectively, during in vitro maturation and in vitro development. Compared with the control group, the rate of blastocyst development significantly increased in the 40 Hz group. In addition, the number of blastocyst cells reduced significantly in the 80 Hz group. In conclusion, the development of blastocysts in cows is facilitated by providing moderate mechanical micro-vibration to immature oocytes and embryos during the in vitro maturation and in vitro development.
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spelling pubmed-58808382018-04-05 Efficacy of mechanical micro-vibration in the development of bovine embryos during in vitro maturation and culture TAKAHASHI, Masahiro HONDA, Tatsutoshi HATOYA, Shingo INABA, Toshio KAWATE, Noritoshi TAMADA, Hiromichi J Vet Med Sci Theriogenology It is currently unclear how mechanical micro-vibration affects the in vitro culture of embryos in Japanese Black cow. In the experimental groups, immature oocytes and fertilized embryos were cultured using the micro-vibration culture system with the vibration set for 5 sec at intervals of 60 min and frequency of 20, 40 or 80 Hz, respectively, during in vitro maturation and in vitro development. Compared with the control group, the rate of blastocyst development significantly increased in the 40 Hz group. In addition, the number of blastocyst cells reduced significantly in the 80 Hz group. In conclusion, the development of blastocysts in cows is facilitated by providing moderate mechanical micro-vibration to immature oocytes and embryos during the in vitro maturation and in vitro development. The Japanese Society of Veterinary Science 2018-02-06 2018-03 /pmc/articles/PMC5880838/ /pubmed/29415921 http://dx.doi.org/10.1292/jvms.17-0607 Text en ©2018 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/)
spellingShingle Theriogenology
TAKAHASHI, Masahiro
HONDA, Tatsutoshi
HATOYA, Shingo
INABA, Toshio
KAWATE, Noritoshi
TAMADA, Hiromichi
Efficacy of mechanical micro-vibration in the development of bovine embryos during in vitro maturation and culture
title Efficacy of mechanical micro-vibration in the development of bovine embryos during in vitro maturation and culture
title_full Efficacy of mechanical micro-vibration in the development of bovine embryos during in vitro maturation and culture
title_fullStr Efficacy of mechanical micro-vibration in the development of bovine embryos during in vitro maturation and culture
title_full_unstemmed Efficacy of mechanical micro-vibration in the development of bovine embryos during in vitro maturation and culture
title_short Efficacy of mechanical micro-vibration in the development of bovine embryos during in vitro maturation and culture
title_sort efficacy of mechanical micro-vibration in the development of bovine embryos during in vitro maturation and culture
topic Theriogenology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5880838/
https://www.ncbi.nlm.nih.gov/pubmed/29415921
http://dx.doi.org/10.1292/jvms.17-0607
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