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Growth Hormone Overexpression Disrupts Reproductive Status Through Actions on Leptin

Growth and reproduction are closely related. Growth hormone (GH)-transgenic common carp exhibit accelerated growth and delayed reproductive development, which provides an amenable model to study hormone cross talk between the growth and reproductive axes. We analyzed the energy status and reproducti...

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Autores principales: Chen, Ji, Cao, Mengxi, Zhang, Aidi, Shi, Mijuan, Tao, Binbin, Li, Yongming, Wang, Yaping, Zhu, Zuoyan, Trudeau, Vance L., Hu, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5880896/
https://www.ncbi.nlm.nih.gov/pubmed/29636726
http://dx.doi.org/10.3389/fendo.2018.00131
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author Chen, Ji
Cao, Mengxi
Zhang, Aidi
Shi, Mijuan
Tao, Binbin
Li, Yongming
Wang, Yaping
Zhu, Zuoyan
Trudeau, Vance L.
Hu, Wei
author_facet Chen, Ji
Cao, Mengxi
Zhang, Aidi
Shi, Mijuan
Tao, Binbin
Li, Yongming
Wang, Yaping
Zhu, Zuoyan
Trudeau, Vance L.
Hu, Wei
author_sort Chen, Ji
collection PubMed
description Growth and reproduction are closely related. Growth hormone (GH)-transgenic common carp exhibit accelerated growth and delayed reproductive development, which provides an amenable model to study hormone cross talk between the growth and reproductive axes. We analyzed the energy status and reproductive development in GH-transgenic common carp by using multi-tissue RNA sequencing, real-time-PCR, Western blotting, ELISA, immunofluorescence, and in vitro incubation. The expression of gys (glycogen synthase) and igfbp1 (insulin-like growth factor binding protein) as well as blood glucose concentrations are lower in GH-transgenic carp. Agrp1 (agouti-related protein 1) and sla (somatolactin a), which are related to appetite and lipid catabolism, are significantly higher in GH-transgenic carp. Low glucose content and increased appetite indicate disrupted metabolic and energy deprivation status in GH-transgenic carp. Meanwhile, the expression of genes, such as gnrhr2 (gonadotropin-releasing hormone receptor 2), gthα (gonadotropin hormone, alpha polypeptide), fshβ (follicle stimulating hormone, beta polypeptide), lhβ [luteinizing hormone, beta polypeptide] in the pituitary, cyp19a1a (aromatase A) in the gonad, and cyp19a1b (aromatase B) in the hypothalamus, are decreased in GH-transgenic carp. In contrast, pituitary gnih (gonadotropin inhibitory hormone), drd1 (dopamine receptor D1), drd3 (dopamine receptor D3), and drd4 (dopamine receptor D4) exhibit increased expression, which were associated with the retarded reproductive development. Leptin receptor mRNA was detected by fluorescence in situ hybridization in the pituitary including the pars intermedia and proximal pars distalis, suggesting a direct effect of leptin on LH. Recombinant carp Leptin protein was shown to stimulate pituitary gthα, fshβ, lhβ expression, and ovarian germinal vesicle breakdown in vitro. In addition to neuroendocrine factors, we suggest that reduced hepatic leptin signaling to the pituitary might be part of the response to overexpression of GH and the resulting delay in puberty onset.
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spelling pubmed-58808962018-04-10 Growth Hormone Overexpression Disrupts Reproductive Status Through Actions on Leptin Chen, Ji Cao, Mengxi Zhang, Aidi Shi, Mijuan Tao, Binbin Li, Yongming Wang, Yaping Zhu, Zuoyan Trudeau, Vance L. Hu, Wei Front Endocrinol (Lausanne) Endocrinology Growth and reproduction are closely related. Growth hormone (GH)-transgenic common carp exhibit accelerated growth and delayed reproductive development, which provides an amenable model to study hormone cross talk between the growth and reproductive axes. We analyzed the energy status and reproductive development in GH-transgenic common carp by using multi-tissue RNA sequencing, real-time-PCR, Western blotting, ELISA, immunofluorescence, and in vitro incubation. The expression of gys (glycogen synthase) and igfbp1 (insulin-like growth factor binding protein) as well as blood glucose concentrations are lower in GH-transgenic carp. Agrp1 (agouti-related protein 1) and sla (somatolactin a), which are related to appetite and lipid catabolism, are significantly higher in GH-transgenic carp. Low glucose content and increased appetite indicate disrupted metabolic and energy deprivation status in GH-transgenic carp. Meanwhile, the expression of genes, such as gnrhr2 (gonadotropin-releasing hormone receptor 2), gthα (gonadotropin hormone, alpha polypeptide), fshβ (follicle stimulating hormone, beta polypeptide), lhβ [luteinizing hormone, beta polypeptide] in the pituitary, cyp19a1a (aromatase A) in the gonad, and cyp19a1b (aromatase B) in the hypothalamus, are decreased in GH-transgenic carp. In contrast, pituitary gnih (gonadotropin inhibitory hormone), drd1 (dopamine receptor D1), drd3 (dopamine receptor D3), and drd4 (dopamine receptor D4) exhibit increased expression, which were associated with the retarded reproductive development. Leptin receptor mRNA was detected by fluorescence in situ hybridization in the pituitary including the pars intermedia and proximal pars distalis, suggesting a direct effect of leptin on LH. Recombinant carp Leptin protein was shown to stimulate pituitary gthα, fshβ, lhβ expression, and ovarian germinal vesicle breakdown in vitro. In addition to neuroendocrine factors, we suggest that reduced hepatic leptin signaling to the pituitary might be part of the response to overexpression of GH and the resulting delay in puberty onset. Frontiers Media S.A. 2018-03-27 /pmc/articles/PMC5880896/ /pubmed/29636726 http://dx.doi.org/10.3389/fendo.2018.00131 Text en Copyright © 2018 Chen, Cao, Zhang, Shi, Tao, Li, Wang, Zhu, Trudeau and Hu. https://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Chen, Ji
Cao, Mengxi
Zhang, Aidi
Shi, Mijuan
Tao, Binbin
Li, Yongming
Wang, Yaping
Zhu, Zuoyan
Trudeau, Vance L.
Hu, Wei
Growth Hormone Overexpression Disrupts Reproductive Status Through Actions on Leptin
title Growth Hormone Overexpression Disrupts Reproductive Status Through Actions on Leptin
title_full Growth Hormone Overexpression Disrupts Reproductive Status Through Actions on Leptin
title_fullStr Growth Hormone Overexpression Disrupts Reproductive Status Through Actions on Leptin
title_full_unstemmed Growth Hormone Overexpression Disrupts Reproductive Status Through Actions on Leptin
title_short Growth Hormone Overexpression Disrupts Reproductive Status Through Actions on Leptin
title_sort growth hormone overexpression disrupts reproductive status through actions on leptin
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5880896/
https://www.ncbi.nlm.nih.gov/pubmed/29636726
http://dx.doi.org/10.3389/fendo.2018.00131
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