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High-Density Genetic Map Construction and Stem Total Polysaccharide Content-Related QTL Exploration for Chinese Endemic Dendrobium (Orchidaceae)
Plants of the Dendrobium genus are orchids with not only ornamental value but also high medicinal value. To understand the genetic basis of variations in active ingredients of the stem total polysaccharide contents (STPCs) among different Dendrobium species, it is of paramount importance to understa...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2018
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5880926/ https://www.ncbi.nlm.nih.gov/pubmed/29636767 http://dx.doi.org/10.3389/fpls.2018.00398 |
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author | Lu, Jiangjie Liu, Yuyang Xu, Jing Mei, Ziwei Shi, Yujun Liu, Pengli He, Jianbo Wang, Xiaotong Meng, Yijun Feng, Shangguo Shen, Chenjia Wang, Huizhong |
author_facet | Lu, Jiangjie Liu, Yuyang Xu, Jing Mei, Ziwei Shi, Yujun Liu, Pengli He, Jianbo Wang, Xiaotong Meng, Yijun Feng, Shangguo Shen, Chenjia Wang, Huizhong |
author_sort | Lu, Jiangjie |
collection | PubMed |
description | Plants of the Dendrobium genus are orchids with not only ornamental value but also high medicinal value. To understand the genetic basis of variations in active ingredients of the stem total polysaccharide contents (STPCs) among different Dendrobium species, it is of paramount importance to understand the mechanism of STPC formation and identify genes affecting its process at the whole genome level. Here, we report the first high-density single-nucleotide polymorphism (SNP) integrated genetic map with a good genome coverage of Dendrobium. The specific-locus amplified fragment sequencing (SLAF-seq) technology led to identification of 7,013,400 SNPs from 1,503,626 high-quality SLAF markers from two parents (Dendrobium moniliforme ♀ × Dendrobium officinale ♂) and their interspecific F(1) hybrid population. The final genetic map contained 8, 573 SLAF markers, covering 19 linkage groups (LGs). This genetic map spanned a length of 2,737.49 cM, where the average distance between markers is 0.32 cM. In total, 5 quantitative trait loci (QTL) related to STPC were identified, 3 of which have candidate genes within the confidence intervals of these stable QTLs based on the D. officinale genome sequence. This study will build a foundation up for the mapping of other medicinal-related traits and provide an important reference for the molecular breeding of these Chinese herb. |
format | Online Article Text |
id | pubmed-5880926 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2018 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-58809262018-04-10 High-Density Genetic Map Construction and Stem Total Polysaccharide Content-Related QTL Exploration for Chinese Endemic Dendrobium (Orchidaceae) Lu, Jiangjie Liu, Yuyang Xu, Jing Mei, Ziwei Shi, Yujun Liu, Pengli He, Jianbo Wang, Xiaotong Meng, Yijun Feng, Shangguo Shen, Chenjia Wang, Huizhong Front Plant Sci Plant Science Plants of the Dendrobium genus are orchids with not only ornamental value but also high medicinal value. To understand the genetic basis of variations in active ingredients of the stem total polysaccharide contents (STPCs) among different Dendrobium species, it is of paramount importance to understand the mechanism of STPC formation and identify genes affecting its process at the whole genome level. Here, we report the first high-density single-nucleotide polymorphism (SNP) integrated genetic map with a good genome coverage of Dendrobium. The specific-locus amplified fragment sequencing (SLAF-seq) technology led to identification of 7,013,400 SNPs from 1,503,626 high-quality SLAF markers from two parents (Dendrobium moniliforme ♀ × Dendrobium officinale ♂) and their interspecific F(1) hybrid population. The final genetic map contained 8, 573 SLAF markers, covering 19 linkage groups (LGs). This genetic map spanned a length of 2,737.49 cM, where the average distance between markers is 0.32 cM. In total, 5 quantitative trait loci (QTL) related to STPC were identified, 3 of which have candidate genes within the confidence intervals of these stable QTLs based on the D. officinale genome sequence. This study will build a foundation up for the mapping of other medicinal-related traits and provide an important reference for the molecular breeding of these Chinese herb. Frontiers Media S.A. 2018-03-27 /pmc/articles/PMC5880926/ /pubmed/29636767 http://dx.doi.org/10.3389/fpls.2018.00398 Text en Copyright © 2018 Lu, Liu, Xu, Mei, Shi, Liu, He, Wang, Meng, Feng, Shen and Wang. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Lu, Jiangjie Liu, Yuyang Xu, Jing Mei, Ziwei Shi, Yujun Liu, Pengli He, Jianbo Wang, Xiaotong Meng, Yijun Feng, Shangguo Shen, Chenjia Wang, Huizhong High-Density Genetic Map Construction and Stem Total Polysaccharide Content-Related QTL Exploration for Chinese Endemic Dendrobium (Orchidaceae) |
title | High-Density Genetic Map Construction and Stem Total Polysaccharide Content-Related QTL Exploration for Chinese Endemic Dendrobium (Orchidaceae) |
title_full | High-Density Genetic Map Construction and Stem Total Polysaccharide Content-Related QTL Exploration for Chinese Endemic Dendrobium (Orchidaceae) |
title_fullStr | High-Density Genetic Map Construction and Stem Total Polysaccharide Content-Related QTL Exploration for Chinese Endemic Dendrobium (Orchidaceae) |
title_full_unstemmed | High-Density Genetic Map Construction and Stem Total Polysaccharide Content-Related QTL Exploration for Chinese Endemic Dendrobium (Orchidaceae) |
title_short | High-Density Genetic Map Construction and Stem Total Polysaccharide Content-Related QTL Exploration for Chinese Endemic Dendrobium (Orchidaceae) |
title_sort | high-density genetic map construction and stem total polysaccharide content-related qtl exploration for chinese endemic dendrobium (orchidaceae) |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5880926/ https://www.ncbi.nlm.nih.gov/pubmed/29636767 http://dx.doi.org/10.3389/fpls.2018.00398 |
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