Cargando…

Expression of RPRM/rprm in the Olfactory System of Embryonic Zebrafish (Danio rerio)

The Reprimo (RPRM) family is composed of highly conserved single-exon genes. The expression pattern of this gene family has been recently described during zebrafish (Danio rerio) embryogenesis, and primarily locates in the nervous system. Its most characterized member, RPRM, which duplicated to give...

Descripción completa

Detalles Bibliográficos
Autores principales: Stanic, Karen, Quiroz, Alonso, Lemus, Carmen G., Wichmann, Ignacio A., Corvalán, Alejandro H., Owen, Gareth I., Opazo, Juan C., Concha, Miguel L., Amigo, Julio D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5881088/
https://www.ncbi.nlm.nih.gov/pubmed/29636669
http://dx.doi.org/10.3389/fnana.2018.00023
_version_ 1783311254802137088
author Stanic, Karen
Quiroz, Alonso
Lemus, Carmen G.
Wichmann, Ignacio A.
Corvalán, Alejandro H.
Owen, Gareth I.
Opazo, Juan C.
Concha, Miguel L.
Amigo, Julio D.
author_facet Stanic, Karen
Quiroz, Alonso
Lemus, Carmen G.
Wichmann, Ignacio A.
Corvalán, Alejandro H.
Owen, Gareth I.
Opazo, Juan C.
Concha, Miguel L.
Amigo, Julio D.
author_sort Stanic, Karen
collection PubMed
description The Reprimo (RPRM) family is composed of highly conserved single-exon genes. The expression pattern of this gene family has been recently described during zebrafish (Danio rerio) embryogenesis, and primarily locates in the nervous system. Its most characterized member, RPRM, which duplicated to give rise rprma and rprmb in the fish lineage, is known to act as a tumor-suppressor gene in mammalian models. Here, we describe in detail the spatiotemporal expression of three rprm genes (rprma, rprmb, and rprml) within distinct anatomical structures in the developing peripheral and central nervous system. In the zebrafish, rprma mRNA is expressed in the olfactory placodes (OP) and olfactory epithelium (OE), rprmb is observed in the tectum opticum (TeO) and trigeminal ganglion (Tg), whereas rprml is found primarily in the telencephalon (Tel). At protein level, RPRM is present in a subset of cells in the OP, and neurons in the OE, TeO, hindbrain and sensory peripheral structures. Most importantly, the expression of RPRM has been conserved between teleosts and mammals. Thus, we provide a reference dataset describing the expression patterns of RPRM gene products during zebrafish and mouse development as a first step to approach the physiological role of the RPRM gene family.
format Online
Article
Text
id pubmed-5881088
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-58810882018-04-10 Expression of RPRM/rprm in the Olfactory System of Embryonic Zebrafish (Danio rerio) Stanic, Karen Quiroz, Alonso Lemus, Carmen G. Wichmann, Ignacio A. Corvalán, Alejandro H. Owen, Gareth I. Opazo, Juan C. Concha, Miguel L. Amigo, Julio D. Front Neuroanat Neuroscience The Reprimo (RPRM) family is composed of highly conserved single-exon genes. The expression pattern of this gene family has been recently described during zebrafish (Danio rerio) embryogenesis, and primarily locates in the nervous system. Its most characterized member, RPRM, which duplicated to give rise rprma and rprmb in the fish lineage, is known to act as a tumor-suppressor gene in mammalian models. Here, we describe in detail the spatiotemporal expression of three rprm genes (rprma, rprmb, and rprml) within distinct anatomical structures in the developing peripheral and central nervous system. In the zebrafish, rprma mRNA is expressed in the olfactory placodes (OP) and olfactory epithelium (OE), rprmb is observed in the tectum opticum (TeO) and trigeminal ganglion (Tg), whereas rprml is found primarily in the telencephalon (Tel). At protein level, RPRM is present in a subset of cells in the OP, and neurons in the OE, TeO, hindbrain and sensory peripheral structures. Most importantly, the expression of RPRM has been conserved between teleosts and mammals. Thus, we provide a reference dataset describing the expression patterns of RPRM gene products during zebrafish and mouse development as a first step to approach the physiological role of the RPRM gene family. Frontiers Media S.A. 2018-03-27 /pmc/articles/PMC5881088/ /pubmed/29636669 http://dx.doi.org/10.3389/fnana.2018.00023 Text en Copyright © 2018 Stanic, Quiroz, Lemus, Wichmann, Corvalán, Owen, Opazo, Concha and Amigo. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Stanic, Karen
Quiroz, Alonso
Lemus, Carmen G.
Wichmann, Ignacio A.
Corvalán, Alejandro H.
Owen, Gareth I.
Opazo, Juan C.
Concha, Miguel L.
Amigo, Julio D.
Expression of RPRM/rprm in the Olfactory System of Embryonic Zebrafish (Danio rerio)
title Expression of RPRM/rprm in the Olfactory System of Embryonic Zebrafish (Danio rerio)
title_full Expression of RPRM/rprm in the Olfactory System of Embryonic Zebrafish (Danio rerio)
title_fullStr Expression of RPRM/rprm in the Olfactory System of Embryonic Zebrafish (Danio rerio)
title_full_unstemmed Expression of RPRM/rprm in the Olfactory System of Embryonic Zebrafish (Danio rerio)
title_short Expression of RPRM/rprm in the Olfactory System of Embryonic Zebrafish (Danio rerio)
title_sort expression of rprm/rprm in the olfactory system of embryonic zebrafish (danio rerio)
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5881088/
https://www.ncbi.nlm.nih.gov/pubmed/29636669
http://dx.doi.org/10.3389/fnana.2018.00023
work_keys_str_mv AT stanickaren expressionofrprmrprmintheolfactorysystemofembryoniczebrafishdaniorerio
AT quirozalonso expressionofrprmrprmintheolfactorysystemofembryoniczebrafishdaniorerio
AT lemuscarmeng expressionofrprmrprmintheolfactorysystemofembryoniczebrafishdaniorerio
AT wichmannignacioa expressionofrprmrprmintheolfactorysystemofembryoniczebrafishdaniorerio
AT corvalanalejandroh expressionofrprmrprmintheolfactorysystemofembryoniczebrafishdaniorerio
AT owengarethi expressionofrprmrprmintheolfactorysystemofembryoniczebrafishdaniorerio
AT opazojuanc expressionofrprmrprmintheolfactorysystemofembryoniczebrafishdaniorerio
AT conchamiguell expressionofrprmrprmintheolfactorysystemofembryoniczebrafishdaniorerio
AT amigojuliod expressionofrprmrprmintheolfactorysystemofembryoniczebrafishdaniorerio