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Changes in growth plate extracellular matrix composition and biomechanics following in vitro static versus dynamic mechanical modulation

The objective of this study was to investigate the effects of mechanical modulation parameters on structural proteins biocomposition and mechanical properties of the growth plate. Establishing these parameters is a crucial step in the development of fusionless treatment of scoliosis. In this study,...

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Autores principales: Kaviani, Rosa, Londono, Irene, Parent, Stefan, Moldovan, Florina, Villemure, Isabelle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Society of Musculoskeletal and Neuronal Interactions 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5881133/
https://www.ncbi.nlm.nih.gov/pubmed/29504583
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author Kaviani, Rosa
Londono, Irene
Parent, Stefan
Moldovan, Florina
Villemure, Isabelle
author_facet Kaviani, Rosa
Londono, Irene
Parent, Stefan
Moldovan, Florina
Villemure, Isabelle
author_sort Kaviani, Rosa
collection PubMed
description The objective of this study was to investigate the effects of mechanical modulation parameters on structural proteins biocomposition and mechanical properties of the growth plate. Establishing these parameters is a crucial step in the development of fusionless treatment of scoliosis. In this study, ulna explants from 4-weeks-old (pubertal) swines were used. The biocomposition was characterized using biochemical content evaluation and immunohistochemistry. Mechanical properties were characterized by fitting the data of the stress relaxation curves using a fibril reinforced biphasic model. For the mechanical loading, one static modulation condition and three different dynamic modulation conditions, with similar average stress but different amplitude and frequency values, were performed using a bioreactor. Results showed that static loading triggers a decrease in proteoglycan content and type X collagen in specific zones of the growth plate. These changes can be associated with the observed decrement of permeability in the static group. None of the three conditions evaluated for dynamic modulation affected the growth plate biocomposition and biomechanical responses. Results of this study provides an improved understanding of growth plate responses to mechanical environment, which will be useful in finding the optimal and non-damaging parameters for fusionless treatments based on the mechanical modulation of bone growth.
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spelling pubmed-58811332018-04-05 Changes in growth plate extracellular matrix composition and biomechanics following in vitro static versus dynamic mechanical modulation Kaviani, Rosa Londono, Irene Parent, Stefan Moldovan, Florina Villemure, Isabelle J Musculoskelet Neuronal Interact Original Article The objective of this study was to investigate the effects of mechanical modulation parameters on structural proteins biocomposition and mechanical properties of the growth plate. Establishing these parameters is a crucial step in the development of fusionless treatment of scoliosis. In this study, ulna explants from 4-weeks-old (pubertal) swines were used. The biocomposition was characterized using biochemical content evaluation and immunohistochemistry. Mechanical properties were characterized by fitting the data of the stress relaxation curves using a fibril reinforced biphasic model. For the mechanical loading, one static modulation condition and three different dynamic modulation conditions, with similar average stress but different amplitude and frequency values, were performed using a bioreactor. Results showed that static loading triggers a decrease in proteoglycan content and type X collagen in specific zones of the growth plate. These changes can be associated with the observed decrement of permeability in the static group. None of the three conditions evaluated for dynamic modulation affected the growth plate biocomposition and biomechanical responses. Results of this study provides an improved understanding of growth plate responses to mechanical environment, which will be useful in finding the optimal and non-damaging parameters for fusionless treatments based on the mechanical modulation of bone growth. International Society of Musculoskeletal and Neuronal Interactions 2018-03 /pmc/articles/PMC5881133/ /pubmed/29504583 Text en Copyright: © Journal of Musculoskeletal and Neuronal Interactions http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Kaviani, Rosa
Londono, Irene
Parent, Stefan
Moldovan, Florina
Villemure, Isabelle
Changes in growth plate extracellular matrix composition and biomechanics following in vitro static versus dynamic mechanical modulation
title Changes in growth plate extracellular matrix composition and biomechanics following in vitro static versus dynamic mechanical modulation
title_full Changes in growth plate extracellular matrix composition and biomechanics following in vitro static versus dynamic mechanical modulation
title_fullStr Changes in growth plate extracellular matrix composition and biomechanics following in vitro static versus dynamic mechanical modulation
title_full_unstemmed Changes in growth plate extracellular matrix composition and biomechanics following in vitro static versus dynamic mechanical modulation
title_short Changes in growth plate extracellular matrix composition and biomechanics following in vitro static versus dynamic mechanical modulation
title_sort changes in growth plate extracellular matrix composition and biomechanics following in vitro static versus dynamic mechanical modulation
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5881133/
https://www.ncbi.nlm.nih.gov/pubmed/29504583
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