SIRFing the replication fork: Assessing protein interactions with nascent DNA

Roy et al. (2018. J. Cell. Biol. https://doi.org/10.1083/jcb.201709121) describe an ingenious single-cell assay system, in situ analysis of protein interactions at DNA replication forks (SIRF), for the quantitative analysis of protein interactions with nascent DNA at active and stalled replication f...

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Detalles Bibliográficos
Autores principales: Branzei, Dana, Giannattasio, Michele
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2018
Materias:
Commentaries
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5881512/
https://www.ncbi.nlm.nih.gov/pubmed/29496736
http://dx.doi.org/10.1083/jcb.201802083
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author Branzei, Dana
Giannattasio, Michele
author_facet Branzei, Dana
Giannattasio, Michele
author_sort Branzei, Dana
collection PubMed
description Roy et al. (2018. J. Cell. Biol. https://doi.org/10.1083/jcb.201709121) describe an ingenious single-cell assay system, in situ analysis of protein interactions at DNA replication forks (SIRF), for the quantitative analysis of protein interactions with nascent DNA at active and stalled replication forks. The sensitive and accurate SIRF methodology is suitable for multiparameter measurements in cell populations.
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spelling pubmed-58815122018-10-02 SIRFing the replication fork: Assessing protein interactions with nascent DNA Branzei, Dana Giannattasio, Michele J Cell Biol Commentaries Roy et al. (2018. J. Cell. Biol. https://doi.org/10.1083/jcb.201709121) describe an ingenious single-cell assay system, in situ analysis of protein interactions at DNA replication forks (SIRF), for the quantitative analysis of protein interactions with nascent DNA at active and stalled replication forks. The sensitive and accurate SIRF methodology is suitable for multiparameter measurements in cell populations. Rockefeller University Press 2018-04-02 /pmc/articles/PMC5881512/ /pubmed/29496736 http://dx.doi.org/10.1083/jcb.201802083 Text en © 2018 Branzei and Giannattasio http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Commentaries
Branzei, Dana
Giannattasio, Michele
SIRFing the replication fork: Assessing protein interactions with nascent DNA
title SIRFing the replication fork: Assessing protein interactions with nascent DNA
title_full SIRFing the replication fork: Assessing protein interactions with nascent DNA
title_fullStr SIRFing the replication fork: Assessing protein interactions with nascent DNA
title_full_unstemmed SIRFing the replication fork: Assessing protein interactions with nascent DNA
title_short SIRFing the replication fork: Assessing protein interactions with nascent DNA
title_sort sirfing the replication fork: assessing protein interactions with nascent dna
topic Commentaries
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5881512/
https://www.ncbi.nlm.nih.gov/pubmed/29496736
http://dx.doi.org/10.1083/jcb.201802083
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AT giannattasiomichele sirfingthereplicationforkassessingproteininteractionswithnascentdna

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