Cargando…

High glucose induces the proliferation of prostatic cells via downregulating MRE11

The aim of the present study was to investigate the candidate genes and pathways associated with benign prostatic hyperplasia (BPH) and diabetes. In vitro experiments were performed using normal prostatic epithelial RWPE-1 and HPr-1 cells. The cell lines were treated with a high-glucose solution and...

Descripción completa

Detalles Bibliográficos
Autores principales: Ye, Chunwei, Cai, Yi, Cai, Qian, Yuan, Shunhui, Huang, Fan, Yang, Xiaofang, He, Shuchen, Li, Zhuoheng, Wang, Yanwen, Yang, Delin, Li, Zhipeng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2018
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5881645/
https://www.ncbi.nlm.nih.gov/pubmed/29532862
http://dx.doi.org/10.3892/ijmm.2018.3549
_version_ 1783311353154371584
author Ye, Chunwei
Cai, Yi
Cai, Qian
Yuan, Shunhui
Huang, Fan
Yang, Xiaofang
He, Shuchen
Li, Zhuoheng
Wang, Yanwen
Yang, Delin
Li, Zhipeng
author_facet Ye, Chunwei
Cai, Yi
Cai, Qian
Yuan, Shunhui
Huang, Fan
Yang, Xiaofang
He, Shuchen
Li, Zhuoheng
Wang, Yanwen
Yang, Delin
Li, Zhipeng
author_sort Ye, Chunwei
collection PubMed
description The aim of the present study was to investigate the candidate genes and pathways associated with benign prostatic hyperplasia (BPH) and diabetes. In vitro experiments were performed using normal prostatic epithelial RWPE-1 and HPr-1 cells. The cell lines were treated with a high-glucose solution and MTS and bromodeoxyuridine assays were used to assess cell viability. Transcriptome sequencing was used to screen the candidate genes. The expression of candidate genes was further verified by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blotting. A meiotic recombination 11 (MRE11) overexpression vector was designed and transfected into RWPE-1 cells to verify the function of MRE11. A streptozotocin-induced diabetic rat model was established and rat MRE11 levels were determined by RT-qPCR and immunohistochemical staining. High concentrations of glucose resulted in RWPE-1 and HPr-1 cells with high viability. Transcriptome sequencing revealed that MRE11 was downregulated when RWPE-1 cells were exposed to high-glucose conditions. When MRE11 was overexpressed, cell viability decreased and cell apoptosis was induced under high-glucose conditions. Prostatic tissues from rats were collected and assessed; MRE11 expression was observed to be decreased, which was consistent with the in vitro cell experiments. BPH may be associated with diabetes, as MRE11 expression in prostatic cells was decreased when exposed to high-glucose conditions. Therefore, MRE11 may have potential as a biomarker for the early diagnosis of BPH and diabetes.
format Online
Article
Text
id pubmed-5881645
institution National Center for Biotechnology Information
language English
publishDate 2018
publisher D.A. Spandidos
record_format MEDLINE/PubMed
spelling pubmed-58816452018-04-12 High glucose induces the proliferation of prostatic cells via downregulating MRE11 Ye, Chunwei Cai, Yi Cai, Qian Yuan, Shunhui Huang, Fan Yang, Xiaofang He, Shuchen Li, Zhuoheng Wang, Yanwen Yang, Delin Li, Zhipeng Int J Mol Med Articles The aim of the present study was to investigate the candidate genes and pathways associated with benign prostatic hyperplasia (BPH) and diabetes. In vitro experiments were performed using normal prostatic epithelial RWPE-1 and HPr-1 cells. The cell lines were treated with a high-glucose solution and MTS and bromodeoxyuridine assays were used to assess cell viability. Transcriptome sequencing was used to screen the candidate genes. The expression of candidate genes was further verified by reverse transcription-quantitative polymerase chain reaction (RT-qPCR) and western blotting. A meiotic recombination 11 (MRE11) overexpression vector was designed and transfected into RWPE-1 cells to verify the function of MRE11. A streptozotocin-induced diabetic rat model was established and rat MRE11 levels were determined by RT-qPCR and immunohistochemical staining. High concentrations of glucose resulted in RWPE-1 and HPr-1 cells with high viability. Transcriptome sequencing revealed that MRE11 was downregulated when RWPE-1 cells were exposed to high-glucose conditions. When MRE11 was overexpressed, cell viability decreased and cell apoptosis was induced under high-glucose conditions. Prostatic tissues from rats were collected and assessed; MRE11 expression was observed to be decreased, which was consistent with the in vitro cell experiments. BPH may be associated with diabetes, as MRE11 expression in prostatic cells was decreased when exposed to high-glucose conditions. Therefore, MRE11 may have potential as a biomarker for the early diagnosis of BPH and diabetes. D.A. Spandidos 2018-06 2018-03-07 /pmc/articles/PMC5881645/ /pubmed/29532862 http://dx.doi.org/10.3892/ijmm.2018.3549 Text en Copyright: © Ye et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Ye, Chunwei
Cai, Yi
Cai, Qian
Yuan, Shunhui
Huang, Fan
Yang, Xiaofang
He, Shuchen
Li, Zhuoheng
Wang, Yanwen
Yang, Delin
Li, Zhipeng
High glucose induces the proliferation of prostatic cells via downregulating MRE11
title High glucose induces the proliferation of prostatic cells via downregulating MRE11
title_full High glucose induces the proliferation of prostatic cells via downregulating MRE11
title_fullStr High glucose induces the proliferation of prostatic cells via downregulating MRE11
title_full_unstemmed High glucose induces the proliferation of prostatic cells via downregulating MRE11
title_short High glucose induces the proliferation of prostatic cells via downregulating MRE11
title_sort high glucose induces the proliferation of prostatic cells via downregulating mre11
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5881645/
https://www.ncbi.nlm.nih.gov/pubmed/29532862
http://dx.doi.org/10.3892/ijmm.2018.3549
work_keys_str_mv AT yechunwei highglucoseinducestheproliferationofprostaticcellsviadownregulatingmre11
AT caiyi highglucoseinducestheproliferationofprostaticcellsviadownregulatingmre11
AT caiqian highglucoseinducestheproliferationofprostaticcellsviadownregulatingmre11
AT yuanshunhui highglucoseinducestheproliferationofprostaticcellsviadownregulatingmre11
AT huangfan highglucoseinducestheproliferationofprostaticcellsviadownregulatingmre11
AT yangxiaofang highglucoseinducestheproliferationofprostaticcellsviadownregulatingmre11
AT heshuchen highglucoseinducestheproliferationofprostaticcellsviadownregulatingmre11
AT lizhuoheng highglucoseinducestheproliferationofprostaticcellsviadownregulatingmre11
AT wangyanwen highglucoseinducestheproliferationofprostaticcellsviadownregulatingmre11
AT yangdelin highglucoseinducestheproliferationofprostaticcellsviadownregulatingmre11
AT lizhipeng highglucoseinducestheproliferationofprostaticcellsviadownregulatingmre11